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World Journal of Microbiology and Biotechnology

, Volume 20, Issue 7, pp 667–672 | Cite as

Induction and partial characterization of intracellular β from Thermoascus aurantiacus and its application in the synthesis of 6-kestose

  • Petros Katapodis
  • Paul Christakopoulos
Article

Abstract

Production of β-fructofuranosidase from the thermophilic fungus Thermoascus aurantiacuswas enhanced by optimization of the type of nitrogen source as well as the type and concentration of carbon source. Submerged batch cultivation in a laboratory bioreactor (7 l) using the optimized medium allowed the production of 85 mU/ml of β-fructofuranosidase. The enzyme showed both transfructosylating and hydrolytic activities and was optimally active at 60 °C and pH 5.0. The enzyme showed the ability to catalyse the synthesis of 1-kestose and the reaction was maximized at 30% (w/v) initial sucrose concentration.

β-Fructofuranosidase 6-kestose Thermoascus aurantiacus 

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References

  1. Andersson, H.B., Ellegard, L.H. & Bosaeus, I.G. 1999 Nondigestibility characteristics of insulin and oligofructose in humans. Journal of Nutrition 129, 1428S–1430S.Google Scholar
  2. Chen, W.C. & Liu, C.H. 1996 Production of b-fructofuranosidase by Aspergillus japonicus. Enzyme and Microbial Technology 18, 153–160.Google Scholar
  3. Duan, K.J., Chen, J.S. & Sheu, D.C. 1994 Kinetic studies and mathematical model for enzymatic production of fructooligosaccharides from sucrose. Enzyme and Microbial Technology 16, 334–339.Google Scholar
  4. Fujita, K., Hara, K., Hashimoto, H. & Kitahata, S. 1990 Purification and some properties of β-fructofuranosidase from Anthrobacter sp. K-1. Agricultural and Biological Chemistry 54, 913–919.Google Scholar
  5. Gupta, A.K. & Bhatia, I.S. 1980 Glucofructosan biosynthesis in Fusarium oxysporum. Phytochemistry 19, 2557–2563.Google Scholar
  6. Hayashi, S., Matsuzaki, K., Takasaki, Y., Ueno, H. & Imada, K. 1992 Production of b-fructofuranosidase by Aspergillus japonicus. World Journal of Microbiology and Biotechnology 8, 155–159.Google Scholar
  7. Hidaka, H., Eida, T. & Saitoh, Y. 1987 Industrial production of fructo-oligosaccharides and its application for human and animals. Nippon Nogeikagaku Kaishi 61, 915–923.Google Scholar
  8. Hidaka, H., Hirayama, H. & Sumi, N. 1988 A fructooligosaccharideproducing enzyme from Aspergillus niger ATCC 20611. Agricultural and Biological Chemistry 52, 1181–1187.Google Scholar
  9. Hirayama M., Sumi N. & Hidaka H. 1989 Purification and properties of a fructooligosaccharide-producing fructofuranosidase from Aspergillus niger ATCC 20611. Agricultural and Biological Chemistry 53, 667–673.Google Scholar
  10. Ivin, P.C. & Clarke, M.L. 1987 Isolation of kestoses and nystose from enzyme digests by high-performance liquid chromatography. Journal of Chromatography 408, 393–398.Google Scholar
  11. Jung, K.H., Lim, J.Y., Yoo, S.L., Lee, J.H. & Yoo, M.Y. 1987 Production of fructosyltransferase from Aureobasidium pullulans. Biotechnology Letters 9, 703–708.Google Scholar
  12. Jung, K.H., Yun, J.W., Kang, K.R., Lim, J.Y. & Lee, J.H. 1989 Mathematical model for enzymatic production of fructo-oligosaccharides from sucrose. Enzyme and Microbial Technology 11, 491–494.Google Scholar
  13. Katapodis, P., Kalogeris, E., Kekos, D., Macris, B.J. & Christakopoulos, P. 2003 Production of b-fructofuranosidase from Sporotrichum thermophile and its application in the synthesis of fructooligosaccharides. Food Biotechnology 17, 1–14.Google Scholar
  14. Kaur, N., Kaur, M., Gupta, A.K. & Singh, R. 1992 Properties of β-fructosidase (Invertase and Inulinase) of Fusarium oxysporum grown on an aqueous extract of Cichorium intybus roots. Journal of Chemical Technology and Biotechnology 53, 279–284.Google Scholar
  15. Kim, B.W., Kwon, H.J., Park, H.Y., Nam, S.W., Park, J.P. & Yun, J.W. 2000 Production of a novel transfructosylating enzyme from Bacillus macerans EG-6. Bioprocess Engineering 23, 11–16.Google Scholar
  16. Kunz, C. & Rudloff, S. 1993 Biological functions of oligosaccharides in human milk. Acta Paediatrica 82, 903–912.Google Scholar
  17. Maheshwari, R., Balasubramanyam, P.V. & Palanivelu, P. 1983 Distinctive behavior of invertase in a thermophilic fungus, Thermomyces lanuginosus. Archives of Microbiology 134, 255–260.Google Scholar
  18. Patel, V., Saunders, G. & Bucke, C. 1994 Production of fructooligosaccharides by Fusarium oxysporum. Biotechnology Letters 11, 1139–1144.Google Scholar
  19. Rivero-Urgell, M. Santamaria-Orleans, A. 2001 Oligosaccharides: application in infant food. Early Human Development 65(Suppl.), S43–S52.Google Scholar
  20. Shiomi, N., Onodera, S., Chatternon, J. & Harrison, P. 1991 Separation of fructooligosaccharide isomers by anion-exchange chromatography. Agricultural and Biological Chemistry 55, 1427–1428.Google Scholar
  21. Wang, X.D. & Rakshit, S.K. 1999 Improved extracellular transferase enzyme production by Aspergillus foetidus for synthesis of oligosaccharides. Bioprocess Engineering 20, 429–434.Google Scholar
  22. Yun, J.W. 1996 Fructooligosaccharides-occurrence, preparation, and application. Enzyme and Microbial Technology 19, 107–117.Google Scholar
  23. Yun, W.J., Jung, K.H., Oh, J.W. & Lee, J.H. 1990 Semi-batch production of fructo-oligosaccharides from sucrose by immobilized cells of Aureobasidium pullulans. Applied Biochemistry and Biotechnology 24/25, 299–308.Google Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Petros Katapodis
    • 1
  • Paul Christakopoulos
    • 1
  1. 1.Biotechnology Laboratory, Chemical Engineering SchoolNational Technical University of AthensGreece

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