World Journal of Microbiology and Biotechnology

, Volume 20, Issue 7, pp 667–672 | Cite as

Induction and partial characterization of intracellular β from Thermoascus aurantiacus and its application in the synthesis of 6-kestose

  • Petros Katapodis
  • Paul Christakopoulos


Production of β-fructofuranosidase from the thermophilic fungus Thermoascus aurantiacuswas enhanced by optimization of the type of nitrogen source as well as the type and concentration of carbon source. Submerged batch cultivation in a laboratory bioreactor (7 l) using the optimized medium allowed the production of 85 mU/ml of β-fructofuranosidase. The enzyme showed both transfructosylating and hydrolytic activities and was optimally active at 60 °C and pH 5.0. The enzyme showed the ability to catalyse the synthesis of 1-kestose and the reaction was maximized at 30% (w/v) initial sucrose concentration.

β-Fructofuranosidase 6-kestose Thermoascus aurantiacus 


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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Petros Katapodis
    • 1
  • Paul Christakopoulos
    • 1
  1. 1.Biotechnology Laboratory, Chemical Engineering SchoolNational Technical University of AthensGreece

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