Abstract
Soil Pb extractability and critical Pb levels in soils were examined for three leafy vegetables grown in artificially Pb-contaminated soils at different Pb concentrations in a greenhouse experiment. Soil Pb extractability was quantified using eight extractants, and the Pb concentration was determined in two different parts of the leafy vegetables (edible parts and roots). The results showed that the extracted Pb concentrations in soils using aqua regia, DTPA, EDTA, 0.5 M HCl, Mehlich-3, and 0.43 M HNO3 had significant (p < 0.01) relationships with Pb concentrations in the edible parts and roots of the leafy vegetables. The results indicated that these extractants were highly suitable for estimating the critical Pb level in soils and describing the maximum allowable level of Pb in leafy vegetables (0.3 mg kg−1). Based on the quality of the linear models (R2 = 0.535–0.649) and the extracted Pb in the soils, we proposed the Mehlich-3 soil test as the standard for soil quality for leafy vegetable production, with the calculated critical level of Pb in soils for growing leafy vegetables, based on this test being 61.39 mg kg−1. Furthermore, the results indicated that Pb is more easily transferred from soil to root than from root to shoot, preventing Pb accumulation in the edible part of leafy vegetables.
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The data used to support the findings of this study are available from the corresponding author upon request.
References
Ahmad, F., & Liu, P. (2020). (Ascorb)ing Pb Neurotoxicity in the developing brain. Antioxidants (Basel), 9(12). https://doi.org/10.3390/antiox9121311
Andrunik, M., Wołowiec, M., Wojnarski, D., Zelek-Pogudz, S., & Bajda, T. (2020). Transformation of Pb, Cd, and Zn minerals using phosphates. Minerals, 10(4). https://doi.org/10.3390/min10040342
Antoniadis, V., Shaheen, S. M., Boersch, J., Frohne, T., Du Laing, G., & Rinklebe, J. (2017). Bioavailability and risk assessment of potentially toxic elements in garden edible vegetables and soils around a highly contaminated former mining area in Germany. Journal of Environmental Management, 186(2), 1–9. https://doi.org/10.1016/j.jenvman.2016.04.036
ATSDR. (2017). Lead toxicity. Retrieved 1 December 2020 from https://www.atsdr.cdc.gov/csem/lead/docs/CSEM-Lead_toxicity_508.pdf
Attanayake, C. P., Hettiarachchi, G. M., Harms, A., Presley, D., Martin, S., & Pierzynski, G. M. (2014). Field evaluations on soil plant transfer of lead from an urban garden soil. Journal of Environmental Quality, 43(2), 475–487. https://doi.org/10.2134/jeq2013.07.0273
Bi, C., Zhou, Y., Chen, Z., Jia, J., & Bao, X. (2018). Heavy metals and lead isotopes in soils, road dust and leafy vegetables and health risks via vegetable consumption in the industrial areas of Shanghai, China. Science of the Total Environment, 619–620, 1349–1357. https://doi.org/10.1016/j.scitotenv.2017.11.177
Bray, R. H., & Kurtz, L. T. (1945). Determination of total, organic, and available forms of phosphorus in soils. Soil Science, 95, 39–45. https://doi.org/10.1097/00010694-194501000-00006
Bremner, J. M., & Keeney, D. R. (1965). Steam distillation methods for determination of ammonium, nitrate and nitrite. Analytica Chimica Acta, 32, 485–495. https://doi.org/10.1016/S0003-2670(00)88973-4
Chen, M., & Ma, L. Q. (2001). Comparison of three aqua regia digestion methods for twenty Florida soils. Soil Science Society of America Journal, 65, 491–499. https://doi.org/10.2136/sssaj2001.652491x
Codex Alimentarius Commission. (2019). General standard for contaminants and toxins in food and feed. Retrieved 28 April from u
Fahr, M., Laplaze, L., Bendaou, N., Hocher, V., Mzibri, M. E., Bogusz, D., & Smouni, A. (2013). Effect of lead on root growth. Frontiers in Plant Science, 4, 1–7. https://doi.org/10.3389/fpls.2013.00175
Fayiga, A. O., & Saha, U. (2016). The effect of bullet removal and vegetation on mobility of Pb in shooting range soils. Chemosphere, 160, 252–257. https://doi.org/10.1016/j.chemosphere.2016.06.098
Finster, M. E., Gray, K. A., & Binns, H. J. (2004). Lead levels of edibles grown in contaminated residential soils: A field survey. Science of the Total Environment, 320(2–3). https://doi.org/10.1016/j.scitotenv.2003.08.009
Ghasemidehkordi, B., Malekirad, A. A., Nazem, H., Fazilati, M., Salavati, H., Shariatifar, N., & Mousavi Khaneghah, A. (2018). Concentration of lead and mercury in collected vegetables and herbs from Markazi province, Iran: A non-carcinogenic risk assessment. Food and Chemical Toxicology, 113, 204–210. https://doi.org/10.1016/j.fct.2018.01.048
Gupta, N., Yadav, K. K., Kumar, V., Kumar, S., Chadd, R. P., & Kumar, A. (2019). Trace elements in soil-vegetables interface: Translocation, bioaccumulation, toxicity and amelioration - A review. Science of the Total Environment, 651, 2927–2942. https://doi.org/10.1016/j.scitotenv.2018.10.047
Gupta, S., & Aten, C. J. I. j. o. e. a. c. (1993). Comparison and evaluation of extraction media and their suitability in a simple model to predict the biological relevance of heavy metal concentrations in contaminated soils. 51(1–4), 25–46.
Herlina, L., Widianarko, B., & Sunoko, H. R. (2020). Phytoremediation potential of cordyline fruticosa for lead contaminated soil. Jurnal Pendidikan IPA Indonesia, 9(1), 42–49. https://doi.org/10.15294/jpii.v9i1.23422
Hong, C. L., Jia, Y. B., Yang, X. E., He, Z. L., & Stoffella, P. J. (2008). Assessing lead thresholds for phytotoxicity and potential dietary toxicity in selected vegetable crops. Bulletin of Environmental Contamination and Toxicology, 80(4), 356–361. https://doi.org/10.1007/s00128-008-9375-z
Hosseiwwnpur, A. R., & Motaghian, H. (2015). Evaluating of many chemical extractants for assessment of Zn and Pb uptake by bean in polluted soils Journal of. Soil Science and Plant Nutrition, 15(1), 24–34. https://doi.org/10.4067/S0718-95162015005000003
Huang, J. W., Chen, J., Berti, W. R., & Cunningham, S. D. (1997). Phytoremediadon of lead-contaminated soils: Role of synthetic chelates in lead phytoextraction. Environmental Science and Technology, 31(3), 800–805. https://doi.org/10.1021/es9604828
ISO-17586. (2016). ISO 17586:2016 Soil quality — Extraction of trace elements using dilute nitric acid. Vol. 14 (Accessed on 11/20/2017).
Kabata-Pendias, A., & Pendias, H. (2001). Trace element in soils and plants, 3rd. CRC Press.
Kashem, M. A., Singh, B. R., Kondo, T., Imamul Huq, S. M., & Kawai, S. (2007). Comparison of extractability of Cd, Cu, Pb and Zn with sequential extraction in contaminated and non-contaminated soils. International Journal of Environmental Science & Technology 4(2). https://doi.org/10.1007/BF03326270
Katoh, M., Masaki, S., & Sato, T. (2012). Single-step extraction to determine soluble lead levels in soil. International Journal of GEOMATE, 3(2), 375–380. https://doi.org/10.21660/2012.6.123
Krailertrattanachai, N., Ketrot, D., & Wisawapipat, W. (2019). The distribution of trace metals in roadside agricultural soils, Thailand. International Journal of Environmental Research and Public Health, 16https://doi.org/10.3390/ijerph16050714
Kunlanit, B., Khwanchum, L., & Vityakon, P. (2020). Land use changes affecting soil organic matter accumulation in topsoil and subsoil in Northeast Thailand. Applied and Environmental Soil Science, 2020, 1–15. https://doi.org/10.1155/2020/8241739
Kushwaha, A., Hans, N., Kumar, S., & Rani, R. (2018). A critical review on speciation, mobilization and toxicity of lead in soil-microbe-plant system and bioremediation strategies. Ecotoxicology and Environmental Safety, 147, 1035–1045. https://doi.org/10.1016/j.ecoenv.2017.09.049
Landrot, G., & Khaokaew, S. (2018). Lead speciation and association with organic matter in various particle-size fractions of contaminated soils. J Environmental Science Technology, 52(12), 6780–6788. https://doi.org/10.1021/acs.est.8b00004
Landrot, G., & Khaokaew, S. (2020). Determining the fate of lead (Pb) and phosphorus (P) in alkaline Pb-polluted soils amended with P and acidified using multiple synchrotron-based techniques. Journal of Hazardous Materials, 399, 123037. https://doi.org/10.1016/j.jhazmat.2020.123037
Lewis, C., Lennon, A. M., Eudoxie, G., Sivapatham, P., & Umaharan, P. (2021). Plant metal concentrations in Theobroma cacao as affected by soil metal availability in different soil types. Chemosphere, 262, 127749. https://doi.org/10.1016/j.chemosphere.2020.127749
Li, Y., & Zhang, M. K. (2013). A comparison of physiologically based extraction test (PBET) and single-extraction methods for release of Cu, Zn, and Pb from mildly acidic and alkali soils. Environmental Science and Pollution Research International, 20(5), 3140–3148. https://doi.org/10.1007/s11356-012-1234-0
Lindsay, W. L., & Norvell, W. A. (1978). Development of a DTPA soil test for zinc, iron, manganese, and copper. Soil Science Society of America Journal. https://doi.org/10.2136/sssaj1978.03615995004200030009x
Liu, X., Peng, K., Wang, A., Lian, C., & Shen, Z. (2010). Cadmium accumulation and distribution in populations of Phytolacca americana L. and the role of transpiration. Chemosphere, 78(9), 1136–1141. https://doi.org/10.1016/j.chemosphere.2009.12.030
Lo, I. M. C., & Yang, X. Y. (1999). EDTA extractation of heavy metals from different soil fractions and synthetic soils. Water, Air, and Soil Pollution, 109, 219–236. https://doi.org/10.1023/A:1005000520321
Luo, C., Yang, R., Wang, Y., Li, J., Zhang, G., & Li, X. (2012). Influence of agricultural practice on trace metals in soils and vegetation in the water conservation area along the East River (Dongjiang River), South China. Science of the Total Environment, 431, 26–32. https://doi.org/10.1016/j.scitotenv.2012.05.027
Lynch, J. (1999). Additional provisional elemental values for LKSD-1, LKSD-2, LKSD-3, LKSD-4, STSD-1, STSD-2, STSD-3 and STSD-4. The Journal of Geostandards and Geoanalysis, 23, 251–260. https://doi.org/10.1111/j.1751-908X.1999.tb00577.x
Mehlich, A. (1984). Mehlich 3 soil test extractant : A modification of Mehlich 2 extractant. Communications in Soil Science and Plant Analysis, 15, 1407–1416.
Minca, K. K., Basta, N. T., & Scheckel, K. G. (2013). Using the mehlich-3 soil test as an inexpensive screening tool to estimate total and bioaccessible lead in urban soils. Journal of Environmental Quality, 42(5), 1518–1526. https://doi.org/10.2134/jeq2012.0450
Nagajyoti, P. C., Lee, K. D., & Sreekanth, T. V. M. (2010). Heavy metals, occurrence and toxicity for plants: A review. Environmental Chemistry Letters, 8, 199–216. https://doi.org/10.1007/s10311-010-0297-8
National Environment Borad. (2021). Notification of the National Environmental Board: Soil quality standard. in the Royal Gazette Retrieved from http://www.ratchakitcha.soc.go.th/DATA/PDF/2564/E/054/T_0020.PDF
Panichsakpatana, S. (1997). Soil pollution from the use of chemicals. Kasetsart University Press.
Pourrut, B., Shahid, M., Dumat, C., Winterton, P., & Pinelli, E. (2011). Lead uptake, toxicity, and detoxification in plants. Reviews of Environmental Contamination and Toxicology, 213, 113–136. https://doi.org/10.1007/978-1-4419-9860-6_4
Przyk, P. E., & Held, A. (2010). Certification of the mass fractions of As, B, Cd, Cr, Cu, Hg, Mn, Mo, Ni, Pb, Sb, Se, Sn and Zn in rye grass, ERM®-CD281. In. Publications Office of the European Union.
Rehman, Z. U., Khan, S., Brusseau, M. L., & Shah, M. T. (2017). Lead and cadmium contamination and exposure risk assessment via consumption of vegetables grown in agricultural soils of five-selected regions of Pakistan. Chemosphere, 168, 1589–1896. https://doi.org/10.1016/j.chemosphere.2016.11.152
Salazar, M. J., Rodriguez, J. H., Nieto, G. L., & Pignata, M. L. (2012). Effects of heavy metal concentrations (Cd, Zn and Pb) in agricultural soils near different emission sources on quality, accumulation and food safety in soybean [Glycine max (L.) Merrill]. Journal of Hazardous Materials, 233–234, 244–253. https://doi.org/10.1016/j.jhazmat.2012.07.026
Salazar, M. J., & Pignata, M. L. (2014). Lead accumulation in plants grown in polluted soils. Screening of native species for phytoremediation. Journal of Geochemical Exploration. https://doi.org/10.1016/j.gexplo.2013.11.003
SEPA. (1995). Environment quality standard for soils. State Environment Protection Administration, China. GB 15618–1995.
Sharma, P., & Dubey, R. S. (2005). Lead toxicity in plants. Brazilian Journal of Plant Physiology, 17(1), 35–52. https://doi.org/10.1590/S1677-04202005000100004
Shiowatana, J., Tantidanai, N., Nookabkaew, S., & Nacapricha, D. (2001). A flow system for the determination of metal speciation in soil by sequential extraction. Environment International, 26(5–6), 381–387. https://doi.org/10.1016/S0160-4120(01)00016-2
Silveira, M. L. A., Alleoni, L. R. F., & Guilherme, L. R. G. J. S. A. (2003). Biosolids and heavy metals in soils. Science in Agriculture, 40, 793–806. https://doi.org/10.1590/S0103-90162003000400029
Soil Survey Staff. (2014). Soil Survey Field and Laboratory Methods Manual. United States Department of Agriculture, Natural Resources Conservation Service. https://doi.org/10.13140/RG.2.1.3803.8889
Soleimani, M., Hajabbasi, M. A., Afyuni, M., Charkhabi, A. H., & Shariatmadari, H. (2009). Bioaccumulation of nickel and lead by Bermuda grass (Cynodon dactylon) and Tall fescue (Festuca arundinacea) from two contaminated soils. Caspian Journal Environmental Science, 7(2), 59–70. https://doi.org/10.4067/S0718-95162015005000003
Solhi, M., Shareatmadari, H., & Hajabbasi, M. A. (2005). Lead and zinc extraction potential of two common crop plants, Helianthus Annuus and Brassica Napus. Water, Air, and Soil Pollution, 167(1–4), 59–71. https://doi.org/10.1007/s11270-005-8089-7
Toth, G., Hermann, T., Da Silva, M. R., & Montanarella, L. (2016). Heavy metals in agricultural soils of the European Union with implications for food safety. Environment International, 88, 299–309. https://doi.org/10.1016/j.envint.2015.12.017
Voyslavov, T., Georgieva, S., Arpadjan, S., & Tsekova, K. (2014). Phytoavailability assessment of cadmium and lead in polluted soils and accumulation byMatricaria chamomilla(Chamomile). Biotechnology & Biotechnological Equipment, 27(4), 3939–3943. https://doi.org/10.5504/bbeq.2013.0038
Wani, A. L., Ara, A., & Usmani, J. A. (2015). Lead toxicity: A review. Interdisciplinary Toxicology, 8(2), 55–64. https://doi.org/10.1515/intox-2015-0009
WHO. (2019, 23 August 2019). Lead poisoning and health. Retrieved 18 December 2020 from https://www.who.int/news-room/fact-sheets/detail/lead-poisoning-and-health
Wu, L., Luo, Y., Xing, X., & Christie, P. (2004). EDTA-enhanced phytoremediation of heavy metal contaminated soil with Indian mustard and associated potential leaching risk. Agriculture, Ecosystems Environment, 102(3), 307–318.
Wu, X., Cai, Q., Xu, Q., Zhou, Z., & Shi, J. (2020). Wheat (Triticum aestivum L.) grains uptake of lead (Pb), transfer factors and prediction models for various types of soils from China. Ecotoxicol Environ Saf, 206, 111387. https://doi.org/10.1016/j.ecoenv.2020.111387
Xiaohai, L., Yuntao, G., Khan, S., Gang, D., Aikui, C., Li, L., & Xuecan, W. (2008). Accumulation of Pb, Cu, and Zn in native plants growing on contaminated sites and their potential accumulation capacity in Heqing, Yunnan. Journal of Environmental Sciences, 20, 1469–1474. https://doi.org/10.1016/S1001-0742(08)62551-6
Xing, W., Liu, H., Banet, T., Wang, H., Ippolito, J. A., & Li, L. (2020). Cadmium, copper, lead and zinc accumulation in wild plant species near a lead smelter. Ecotoxicology and Environmental Safety, 198https://doi.org/10.1016/j.ecoenv.2020.110683
Yoon, J., Cao, X., Zhou, Q., & Ma, L. Q. (2006). Accumulation of Pb, Cu, and Zn in native plants growing on a contaminated Florida site. Science of the Total Environment, 368(2–3), 456–464. https://doi.org/10.1016/j.scitotenv.2006.01.016
Zhang, S., Song, J., Cheng, Y., & Lv, M. (2018). Proper management of lead-contaminated agricultural lands against the exceedance of lead in agricultural produce: Derivation of local soil criteria. Science of the Total Environment, 634, 321–330. https://doi.org/10.1016/j.scitotenv.2018.03.337
Zulfiqar, U., Farooq, M., Hussain, S., Maqsood, M., Hussain, M., Ishfaq, M., & Anjum, M. Z. (2019). Lead toxicity in plants: Impacts and remediation. Journal of Environmental Management, 250, 109557. https://doi.org/10.1016/j.jenvman.2019.109557
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This research was supported in part by the Graduate Program Scholarship from The Graduate School, Kasetsart University, Bangkok, Thailand.
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All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Kanokrada Kongtawee and Daojarus Ketrot. The first draft of the manuscript was written by Kanokrada Kongtawee and Daojarus Ketrot. All authors commented on previous versions of the manuscript and read and approved the final manuscript.
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Kongtawee, K., Ketrot, D., Wisawapipat, W. et al. Assessing Critical Level of Lead in Soils for Leafy Vegetables. Water Air Soil Pollut 233, 459 (2022). https://doi.org/10.1007/s11270-022-05937-7
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DOI: https://doi.org/10.1007/s11270-022-05937-7