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Effects of Glyphosate on Somatic and Ovarian Growth in the Estuarine Crab Neohelice granulata, During the Pre-Reproductive Period

Abstract

Adult females of the estuarine crab Neohelice granulata were exposed during the 3-month pre-reproductive period (winter) to the herbicide glyphosate, the most used pesticide in Argentina, at three different concentrations (0.02, 0.2, and 1 mg/L, as active ingredient). At the end of the in vivo assay, the body weight gain and the ovarian growth were estimated, the last one in terms of the gonadosomatic index (GSI), the relative proportion of the different kind of oocytes, and their relative size. A decrease in the body weight gain was observed by effect of pure glyphosate, at all concentrations assayed. Although no differences in either the GSI or vitellogenic protein content of the ovary were noted between any glyphosate concentration and control, a higher proportion of reabsorbed vitellogenic oocytes was observed in the ovaries of crabs exposed to glyphosate at 1 mg/L, together with an increased area of previtellogenic oocytes. These effects were confirmed in vitro, at a glyphosate concentration of 0.2 mg/L. In fact, a higher area of previtellogenic oocytes was seen when glyphosate was added to the culture medium containing ovarian tissue, but a significant higher incidence of reabsorbed vitellogenic oocytes was seen only when eyestalk tissue was also added to the vials, suggesting that the secretion of some neurohormone involved in reabsorption is enhanced. The obtained results indicate that glyphosate is able to harm, in the studied species, both somatic and the ovarian growth.

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Acknowledgements

This study was supported by grants from both CONICET (PIP2015, code 11220150100100CO) and the University of Buenos Aires (UBACYT 2016 scientific program, code 20020150100060BA).

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Correspondence to Enrique M. Rodríguez.

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Avigliano, L., Canosa, I.S., Medesani, D.A. et al. Effects of Glyphosate on Somatic and Ovarian Growth in the Estuarine Crab Neohelice granulata, During the Pre-Reproductive Period. Water Air Soil Pollut 229, 44 (2018). https://doi.org/10.1007/s11270-018-3698-0

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  • DOI: https://doi.org/10.1007/s11270-018-3698-0

Keywords

  • Estuarine crabs
  • Glyphosate
  • Reproduction
  • Ovarian growth
  • Oocytes