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Rhizobacterial Pseudomonas spp. Strains Harbouring acdS Gene Could Enhance Metallicolous Legume Nodulation in Zn/Pb/Cd Mine Tailings

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Abstract

Phytostabilisation can benefit from phytostimulatory rhizobacteria. Forty-three bacterial strains were isolated from the roots of the metallicolous legume Anthyllis vulneraria ssp. carpatica grown in a highly contaminated mine tailing (total Cd, Pb and Zn were up to 1200; 34,000; and 170,000 mg kg−1, respectively). We aimed at evaluating their phytostimulatory effects on the development of leguminous metallophytes. Strains were screened for fluorescent siderophores and auxin synthesis, inorganic P solubilisation and 1-amino-cyclopropane-1-carboxylate deaminase (ACCd) activity to define a subset of 11 strains that were inoculated on the leguminous metallophytes A. vulneraria and Lotus corniculatus grown in diluted mine spoil (Zn 34,653; Pb 6842; and Cd 242, all in mg kg−1). All strains were affiliated to Pseudomonas spp. (except two), synthetised auxins and siderophores and solubilised P (except three), and seven of them were ACCd positive. The inoculation effects (shoot-root-nodule biomass, chlorophyll content) depended on legume species and bacterial strain genotype. Phytostimulation scores were unrelated to siderophore/auxin synthesis and P solubilisation rates. Inoculations of the strain nos. 17–43 triggered a 1.2-fold significant increase in the chlorophyll content of A. vulneraria. Chlorophyll content and root biomass of L. corniculatus were significantly increased following the inoculations of the strain nos. 17–22 (1.5–1.4-fold, respectively). The strongest positive effects were related to increases in the nodule biomass of L. corniculatus in the presence of three ACCd-positive strains (1.8-fold), one of which was the highest auxin producer. These data suggest to focus on interactions between ACCd activity and auxin synthesis to enhance nodulation of metallicolous legumes.

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Acknowledgements

Financial support was provided by the ANR project SyMetal (grant number ANR-10-CESA-0006). The authors wish to thank M. Guy Delmot for his technical assistance on the experimental site without which the implementation of the phytostabilisation assays would not have been feasible.

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Authors and Affiliations

  1. IRD, UMR LSTM, 34398, Montpellier, France

    Souhir Soussou & Ezékiel Baudoin

  2. Montpellier SupAgro, UMR LSTM, 34398, Montpellier, France

    Brigitte Brunel

  3. INRA, UMR LSTM, 34398, Montpellier, France

    Marjorie Pervent & Jean-Claude Cleyet-Marel

  4. INRA, UMR Agroecology, 21065, Dijon, France

    Diederik van Tuinen

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  1. Souhir Soussou
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Soussou, S., Brunel, B., Pervent, M. et al. Rhizobacterial Pseudomonas spp. Strains Harbouring acdS Gene Could Enhance Metallicolous Legume Nodulation in Zn/Pb/Cd Mine Tailings. Water Air Soil Pollut 228, 142 (2017). https://doi.org/10.1007/s11270-017-3309-5

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