Effect of Elevated UV-B Radiation on Microbial Biomass C and Soil Respiration in Different Barley Cultivars Under Field Conditions



Elevated ultraviolet-B (UV-B) radiation effect on soil carbon (C) cycling has been regarded as one of the important issues in global change. Field simulating experiment was conducted to investigate the effects of elevated UV-B radiation on the content of microbial biomass C in rhizosphere and non-rhizosphere soils as well as soil respiration in three barley cultivars. The UV-B radiation was set at two levels, i.e., reference (A, ambient UV-B radiation), and elevated by 20% (E, elevated UV-B radiation, 14.4 kJ m−2 day−1). Three barley cultivars were tested including Dan 2, Supi 3, and Supi 4, respectively. The results indicated that elevated UV-B radiation obviously decreased the content of microbial biomass C in rhizosphere and non-rhizosphere soil, but had no effect on the tendency of microbial biomass C in rhizosphere and non-rhizosphere soil during the entire barley growing season. Compared with control (ambient UV-B radiation), elevated UV-B radiation depressed soil respiration rate and its temperature sensitivity coefficients (Q10), especially in barley cultivar Dan 2 and cultivar Supi 3. It is suggested that the different responses of three barley cultivars to elevated UV-B radiation were related to the changes of microbial biomass C and soil respiration.


UV-B radiation Barley Rhizosphere Non-rhizosphere Microbial biomass C Q10 


  1. Chen, S. T., Hu, Z. H., & Li, H. M. (2009). Temperature sensitivity of wheat plant respiration and soil respiration influenced by increased UV-B radiation from elongation to flowering periods [J]. Chinese Journal of Environmental Science, 30(5), 1249–1254.Google Scholar
  2. Fang, C., & Moncrieff, J. B. (2001). The dependence of soil CO2 efflux on temperature [J]. Soil Biology and Biochemistry, 33(2), 155–165.CrossRefGoogle Scholar
  3. Han, Y., Lou, Y. S., Han, C., Li, M., & Hu, C. D. (2015). Effects of potassium supply on physiological properties in peanut under enhanced ultraviolet-B radiation [J]. Fresenius Environmental Bulletin, 24, 3831–3835.Google Scholar
  4. Hou, F. J., & Ben, G. Y. (1999). Effects of elevated UV-B radiation on physiological properties in the seedlings of soybean and cucumber [J]. Chinese Journal of Applied & Environmental Biology, 5(5), 455–458.Google Scholar
  5. Hu, Z. H., Jiang, J. Y., & Niu, C. P. (2009). Impacts of enhanced ultraviolet-B radiation on CO2 emission from soil-soybean system [J]. China Environmental Science, 29(4), 425–430.Google Scholar
  6. Hu, Z. H., Yang, Y. P., & Chen, S. T. (2010). Combined effect of enhanced UV-B radiation and straw addition on CO_2 emission from soil-winter wheat system [J]. China Environmental Science, 30(8), 1130–1134.Google Scholar
  7. Kerril, B. (1994). Evidence for large upward trends of ultraviolet-B radiation lined to ozone depletion [J]. Science, 262(5136), 1032–1034.CrossRefGoogle Scholar
  8. Li, D. B., Wu, Z. J., & Chen, L. J. (2004). Dynamics of microbial biomass C in a black soil under long-term fertilization and related affecting factors [J]. Chinese Journal of Applied Ecology, 15(8), 1334–1338.Google Scholar
  9. Lou, Y. S., Cheng, H. Y., & Wang, E. J. (2010). Effects of enhanced ultraviolet-B radiation and nitrogen levels on microbial biomass carbon and nitrogen of barley [J]. Chinese Agricultural Science Bulletin, 26(13), 219–224.Google Scholar
  10. Lou, Y. S., Huang, Y., & Li, Y. X. (2011). Effect of enhanced ultraviolet-B radiation on physiological properties of different cultivars of barley [J]. Chinese Journal of Rural Eco-Environment, 27(4), 51–55.Google Scholar
  11. Lu, R. K. (1999). Analytical methods for soils and agro-chemistry [M]. Beijing: China Agriculture Press.Google Scholar
  12. Nsabimana, D., Haynes, R. J., & Wallis, F. M. (2004). Size, activity and catabolic diversity of the soil microbial biomass as affected by land use [J]. Applied Soil Ecology, 26(2), 81–92.CrossRefGoogle Scholar
  13. Pal, M., Zaidi, P. H., & Voleti, S. R. (2006). Solar UV-B exclusion effects on growth and photosynthetic characteristics of wheat and pea [J]. Journal of New Seeds, 8(1), 19–34.CrossRefGoogle Scholar
  14. Pandey, J., & Chaplot, K. (2007). Effects of enhanced UV-B radiation on physiological and biochemical characteristics of wheat [J]. Research on Crops, 8(2), 401–405.Google Scholar
  15. Powlson, D. S., Brookes, P. C., & Christensen, B. T. (1987). Measurement of soil microbial biomass provides an early indication of changes in total soil organic matter due to straw incorporation [J]. Soil Biology and Biochemistry, 19(2), 159–164.CrossRefGoogle Scholar
  16. Ravishankara, A. R., Daniel, J. S., & Portmann, R. W. (2009). Nitrous Oxide (N2O): the dominant ozone-depleting substance emitted in the 21st century [J]. Science, 326(5949), 123–125.CrossRefGoogle Scholar
  17. Wang, Y. S., & Wang, Y. H. (2003). Quick measurement of CH4, CO2 and N2O emissions from a short-time ecosystem [J]. Advances in Atmospheric Sciences, 20(5), 842–844.CrossRefGoogle Scholar
  18. Wang, S. B., Su, W. H., & Wei, D. W. (1993). Biologically effective radiation of solar ultraviolet radiation and the depletion of ozone layer. Acta Scientiae Circumstantiae, 13(1), 114–119.Google Scholar
  19. Wang, C. H., Zheng, Y. F., & He, D. L. (2003). The sensitivity of wheat different parameters in response to elevated UV-B radiation [J]. Chinese Agricultural Science Bulletin, 19(6), 43–45.Google Scholar
  20. Wu, J., Lou, Y. S., & Li, Y. (2010). Effects of elevated UV-B radiation on physiological and ecological properties in barley [J]. Chinese Journal of Agro-Environmental Sciences, 29(6), 1033–1038.Google Scholar
  21. Zelles, L. (1999). Fatty acid patterns of phospholipids and lipopolysaccharides in the characterization of microbial communities in soil: a review [J]. Biology and Fertility of Soils, 29(2), 111–129.CrossRefGoogle Scholar
  22. Zhang, C. E., & Liang, Y. L. (2001). Effect of different amounts of nitrogen and phosphorus fertilizers applied on soil microbial biomass during corn growth periods [J]. Chinese Journal of Eco-Agriculture, 9(2), 72–74.Google Scholar
  23. Zheng, Y. F., Yan, J. Y., & Wan, C. J. (1996). Effects of elevated UV-B radiation on crops and its measures [J]. Chinese Journal of Agrometeorology, 17(4), 50–54.Google Scholar
  24. Zhou, Q., Huang, X. H., & Ma, Y. G. (2001). Effect of elevated UV-B radiation on wheat growth [J]. Agro-Environmental Protection, 20(2), 94–96.Google Scholar

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© Springer International Publishing Switzerland 2017

Authors and Affiliations

  1. 1.Collaborative Innovation Center on Forecast and Evaluation of Meteorological DisastersNanjing University of Information Science and TechnologyNanjingChina
  2. 2.Jiangsu Key Laboratory of Agricultural MeteorologyNanjing University of Information Science and TechnologyNanjingChina

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