Water, Air, & Soil Pollution

, 228:10

Enrichment of Anammox Biomass from Different Seeding Sludge: Process Strategy and Microbial Diversity

  • Zhiji Ding
  • Valeria Ventorino
  • Antonio Panico
  • Olimpia Pepe
  • Eric D. van Hullebusch
  • Francesco Pirozzi
  • Isabelle Bourven
  • Gilles Guibaud
  • Giovanni Esposito
Article
  • 238 Downloads

Abstract

The current study aims to tackle one of the main obstacles in the application of anaerobic ammonium oxidation (Anammox) technology, i.e., the extreme slow growth of the Anammox bacteria. Three conventional sludge has been tested in sequencing batch reactor for Anammox enrichment, including conventional aerobic sludge, denitrification sludge, and anaerobic sludge. With a high selection stress and insufficient oxygen control, the reactor seeded with aerobic sludge reached 50–60% total nitrogen removal after 240 days whereas that seeded with anaerobic sludge failed to establish Anammox activity. Anammox process was successfully established in the reactor seeded with denitrification sludge with a total nitrogen removal of approximately 80% after 150 days under strict oxygen control (DO <0.2 mg/L) and low selection stress. Under the same operational condition, the reactor seeded with anaerobic sludge reached only 20–30% total nitrogen removal. All the reactors experienced fluctuating performances during the enrichment process, which was believed to be the consequence of inhibitory factors such as dissolved oxygen, nitrite and free ammonia as well as undesirable coexisting bacteria which compete for the same substrate. The denaturing gradient gel electrophoresis (DGGE) band from the amplified DNA samples extracted from different enrichment stage showed a clear evolution of the microbial composition as reflected by the change in the band locations and their intensity.

Keywords

Nitrogen Anammox Ammonium Nitrite 

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Zhiji Ding
    • 1
  • Valeria Ventorino
    • 2
  • Antonio Panico
    • 3
  • Olimpia Pepe
    • 2
  • Eric D. van Hullebusch
    • 4
  • Francesco Pirozzi
    • 3
  • Isabelle Bourven
    • 5
  • Gilles Guibaud
    • 5
  • Giovanni Esposito
    • 1
  1. 1.Department of Civil and Mechanical EngineeringUniversity of Cassino and Southern LazioCassinoItaly
  2. 2.Division of Microbiology, Department of AgricultureUniversity of Naples Federico IIPorticiItaly
  3. 3.Department of Civil, Architectural and Environmental EngineeringUniversity of Naples Federico IINaplesItaly
  4. 4.Laboratoire Géomatériaux et Environnement (EA 4508)Université Paris-Est, UPEMMarne-la-ValléeFrance
  5. 5.Groupement de Recherche Eau Sol EnvironnementUniversité de LimogesLimogesFrance

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