Abstract
The cell density-dependent gene expression in gram-negative bacteria is through the activity of acyl homoserine lactone signal molecules. The novel strain Pseudomonas putida JMQS1 isolated from detergent-contaminated soil exhibited quorum sensing along with its ability to degrade phenol. The response to Chromobacterium violaceum DSTS-1 mutant biosensor and luxI and luxR gene-specific amplification was used to characterize the quorum sensing property of the isolate. A regulation in the synthesis of various acyl homoserine lactone molecules, viz C6HSL in the initial stages of phenol stress, C8HSL during degradation, and 3OC12HSL on completion of degradation could be identified by liquid chromatography-quadrupole time of flight. Thin-layer chromatography, Fourier transform infrared spectroscopy, and gas chromatography mass spectrometry confirmed the complete degradation of phenol in 48–56 h. P. putida JMQS1 exhibited adaptation over phenol stress through the selective activation of the quorum sensing signal molecules depending on the changing physiological conditions. This study proposes an efficient method for enhancing the degradation of toxic organic pollutants by the supplementation of acyl homoserine lactone signal molecules.
Similar content being viewed by others
References
Antony, M., Mohammed, A. S., Salim, S., Sajudeen, P. A., Nair, I. C., & Jayachandran, K. (2013). Inhibition of violacein synthesis in Chromobacterium violaceium DSTS-1 mutant. International Journal of Advanced Biotechnology and Research, 4(1), 1014–1020.
Bratu, S., Gupta, J., & Quale, J. (2006). Expression of the las and rhl quorum-sensing systems in clinical isolates of Pseudomonas aeruginosa does not correlate with efflux pump expression or antimicrobial resistance. Journal of Antimicrobial Chemotherapy, 58, 1250–1253. http://dx.doi.org/10.1093/jac/dkl407.
Nde, C. W., Jang, H., Toghrol, F., & Bentley, W. E. (2008). Toxicogenomic response of Pseudomonas aeruginosa ortho-phenylphenol. BMC Genomics, 9, 473. doi:10.1186/1471-2164-9-473.
Chong, T. M., Koh, C. L., Sam, C. K., Choo, Y. M., Yin, W. F., & Chan, K. G. (2012). Characterization of quorum sensing and quorum quenching soil bacteria isolated from Malaysian tropical montane forest. Sensors, 12, 4846–4859. http://dx.doi.org/10.3390/s120404846.
Chowdhury, A. M. A. Z., & Fakhruddin, A. N. M. (2010). Phenol biodegradation by Pseudomonas putida CP1 and A(a). Proceedings of ICEAB, 10, 155–158.
da Silva, G. A., & de Almeida, E. A. (2006). Production of yellow-green fluorescent pigment by Pseudomonas fluorescens. Brazilian Archives of Biology and Technology, 49, 411–419. http://dx.doi.org/10.1590/S1516-89132006000400009.
Fakhruddin, A. N. M., & Quilty, B. (2006). The response of Pseudomonas putida CP1 cells to nutritional, chemical and environmental stresses. World Journal of Microbiology and Biotechnology, 22, 507–514. http://dx.doi.org/10.1007/s11274-005-9064-6.
Kiprop, A. K., Pourtier, E., & Kimutai, S. K. (2013). GC-MS and ESI-MS detection of catechol. International Journal of Educational Research, 1(12), 1–12.
Mordocco, A., Clern, K., & Roger, J. (1999). Continuous degradation of phenol at low concentration using immobilized Pseudomonas putida. Enzyme and Microbial Technology, 25, 530–536. http://dx.doi.org/10.1016/S0141-0229(99)00078-2.
Nair, I. C., Jayachandran, K., & Shashidhar, S. (2007). Treatment of phenol containing paper factory effluent with immobilized cells of phenol degrading Alcaligenes sp. Bioresource Technology, 98, 714–716. http://dx.doi.org/10.1016/j.biortech.2006.02.034.
Nair, I. C., Jayachandran, K., & Shashidhar, S. (2008). Biodegradation of phenol. African Journal of Biotechnology, 7, 4951–4958. http://dx.doi.org/10.5897/AJB08.087.
Nair, I. C., Pradeep, S., Ajayan, M. S., Jayachandran, K., & Shashidhar, S. (2009). Accumulation of intracellular Polyhydroxybutyrate in Alcaligenes sp d2 under phenol stress. Applied Biochemistry and Biotechnology, 159(2), 545–552.
Pradeep, N. V., Anupama, S., Navya, K., Shalini, H. N., Idris, M., & Hampannavar, U. S. (2015). Biological removal of phenol from waste waters: a mini review. Applied Water Science, 5, 105–112. http://dx.doi.org/10.1007/s13201-014-0176-8.
Shaw, P. D., Ping, G., Daly, S. L., Cha, C., Cronan, J. E., Jr., & Rinehart, K. L. (1997). Detecting and characterizing N-acyl-homoserine lactone signal molecules by thin layer chromatography. Proceedings of the National Academy of Sciences of the United States of America, 94, 6036–6041.
Silverstein, R. M., & Webster, F. X. (1998). Spectrometric identification of organic compounds (6th ed.). New York: John Wiley & Sons, Inc.
Solomons, T. W. G., & Fryhle, C. (2007). Organic Chemistry (10th ed.). New York: John Wiley.
Steindler, L., Bertani, I., Luisa, D. S., Bigirimana, J., & Venturi, V. (2008). The presence, type and role of N-acyl homoserine lactone quorum sensing in fluorescent Pseudomonas originally isolated from rice rhizospheres are unpredictable. FEMS Microbiology Letters, 288, 102–111. http://dx.doi.org/10.1111/j.1574-6968.2008.01344.x.
Val, D. L., & Cronan, J. E., Jr. (1998). In vivo evidence that S-adenosylmethionine and fatty acid synthesis intermediates are the substrates for the LuxI family of autoinducer synthases. Journal of Bacteriology, 180(10), 2644–2651.
Venturi, V. (2006). Regulation of quorum sensing in Pseudomonas. FEMS Microbiology Reviews, 30, 274–291. http://dx.doi.org/10.1111/j.1574-6976.2005.00012.x274-291.
Veselova, M. A. (2010). Quorum sensing regulation in Pseudomonas. Genetika, 46, 149–158. http://dx.doi.org/10.1134/S1022795410020018.
Wilson, K. (1997). Preparation of Genomic DNA from Bacteria. Current Protocols in Molecular Biology, 2.4.1-2.4.5.
Yong, Y. C., & Zhong, J. J. (2013). Regulation of aromatics biodegradation by rhl quorum sensing through induction of catechol meta-cleavage pathway. Bioresource Technology, 136, 761–765. http://dx.doi.org/10.1016/j.biortech.2013.03.134.
Acknowledgments
We are grateful to Kerala State Council for Science, Technology & Environment (KSCSTE) for their research fellowships and grants through Young Investigators Programme in Biotechnology (YIPB) for this work. We thank DBT-MSUB Analytical Test Facility and the University Common Instrumentation Centre, Mahatma Gandhi University, for their support in this venture.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Funding
This work was supported by Kerala State Council for Science, Technology and Environment, Government of Kerala, Trivandrum. Council (P) Order No.185/2012/KSCSTE.
Conflict of Interest
The authors declare that they have no conflict of interest.
Human and Animal Rights
This article does not contain any studies with human participants or animals performed by any of the authors.
Rights and permissions
About this article
Cite this article
Antony, M., Jayachandran, K. Regulation of Acyl Homoserine Lactone Synthesis in Pseudomonas putida JMQS1 Under Phenol Stress. Water Air Soil Pollut 227, 338 (2016). https://doi.org/10.1007/s11270-016-3018-5
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s11270-016-3018-5