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Tributyltin (TBT) Tolerance of Indigenous and Non-indigenous Bacterial Species

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Abstract

Tributyltin (TBT) is a species of organotin compound (OTC), used as antifouling biocide in boat and ship paints to prevents the attachment of marine organism on their hull surfaces. Tributyltin was found to be very toxic to a variety of targeted and non-targeted organisms and has high persistence in sediments even after the total global ban by the International Maritime Organization (IMO) in 2008. Therefore, there is an urgent need to clean up TBT-polluted environments after the global banning due to the significant risks it poses to the human and aquatic organisms for its slow degradation rate. In selecting bioremediation agents, indigenous bacteria were documented to be of great potentials compared to non-indigenous. In this study, comparison was made between a bacterial isolate Klebsiella sp. FIRD 2, isolated from TBT-contaminated surface sediment and Pseudomonas specie isolated from non-TBT-contaminated soil. Previously, we isolated, screened, and identified Klebsiella sp. FIRD 2 as a TBT-resistant bacterium from TBT-contaminated surface sediment of Kong Kong Laut, Johor, Malaysia. The isolate was able to resist TBT up to 1500 μg/L without addition of carbon source in minimal salt medium (MSM). Pseudomonas sp., isolated from non-TBT-contaminated soil was tested in MSM treated with different concentration of TBT. The bacterium did not endure to survive in TBT-treated media without addition of carbon source; thus, the strain has no ability to utilize TBT as source carbon. Growth of Pseudomonas sp. was observed in MSM treated with TBT at concentration of 500 μg/L and 1000 μg/L along with addition of glucose as carbon source. No growth of Pseudomonas sp. was observed in MSM with higher TBT concentration even with additional of carbon source. This study equally endorses the potentials of indigenous bacteria in bioremediation of TBT contamination.

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References

  • Abubakar, A, MuskhazliB, M, Lutfi, WJ, Syaizwan, ZZ, Ahmad, I, Ferdaus, B-Y. (2015). Klebsiella sp. FIRD 2, a TBT-resistant bacterium isolated from contaminated surface sediment along Strait of Johor Malaysia. Marine Pollution Bulletin journal, 101(1), 280–283. doi:http://dx.doi.org/10.1016/j.marpolbul.2015.09.041

  • Adelaja, O. A., & Keenan, H. E. (2012). Tolerance of TBT-resistant bacteria isolates to methylmercury. Reasearch Jounal of Environmental Science, 6(1), 1–13.

    Article  CAS  Google Scholar 

  • Antizar-Ladislao, B. (2008a). Environmental levels, toxicity and human exposure to tributyltin (TBT)-contaminated marine environment. a review. b_antizar@hotmail.com. Environment International, 34(2), 292–308. doi:10.1016/j.envint.2007.09.005.

    Article  CAS  Google Scholar 

  • Antizar-Ladislao, B. (2008b). Environmental levels, toxicity and human exposure to tributyltin (TBT)-contaminated marine environment. A review. Environmental International, 34(2), 292–308.

    Article  CAS  Google Scholar 

  • Bernat, P., Siewiera, P., Soboń, A., & Długoński, J. (2014). Phospholipids and protein adaptation of Pseudomonas sp. to the xenoestrogen tributyltin chloride (TBT). World Journal of Microbiology and Biotechnology, 30(9), 2343–2350. doi:10.1007/s11274-014-1659-3.

    Article  CAS  Google Scholar 

  • Berto, D., Giani, M., Boscolo, R., Covelli, S., Giovanardi, O., Massironi, M., & Grassia, L. (2007). Organotins (TBT and DBT) in water, sediments, and gastropods of the southern Venice lagoon (Italy). Marine Pollution Bulletin, 55(10-12), 425–35. doi:10.1016/j.marpolbul.2007.09.005.

    Article  CAS  Google Scholar 

  • Bettin, C., Oehlmann, J., & Stroben, E. (1996). TBT-induced imposex in marine neogastropods is mediated by an increasing androgen level. Helgoländer Meeresuntersuchungen, 50(3), 299–317. doi:10.1007/BF02367105.

    Article  Google Scholar 

  • Cruz, A., Anselmo, A. M., Suzuki, S., & Mendo, S. (2015). Tributyltin (TBT): a review on microbial resistance and degradation. Critical Reviews in Environmental Science and Technology, 45(9), 970–1006. doi:10.1080/10643389.2014.924181.

    Article  CAS  Google Scholar 

  • Cruz, A., Caetano, T., Suzuki, S., & Mendo, S. (2007). Aeromonas veronii, a tributyltin (TBT)-degrading bacterium isolated from an estuarine environment, Ria de Aveiro in Portugal. Marine Environmental Research, 64(5), 639–50. doi:10.1016/j.marenvres.2007.06.006.

    Article  CAS  Google Scholar 

  • Cruz, A., Henriques, I., Sousa, A. C. A., Baptista, I., Almeida, A., Takahashi, S., et al. (2014). A microcosm approach to evaluate the degradation of tributyltin (TBT) by Aeromonas molluscorum Av27 in estuarine sediments. Environmental Research, 132, 430–437. doi:10.1016/j.envres.2014.04.031.

    Article  CAS  Google Scholar 

  • Cruz, A, Moreira, R, Mendo, S. (2013). An easy, rapid and inexpensive method to monitor tributyltin (TBT) toxicity in the laboratory. Folia microbiologica, 0–4. doi:10.1007/s12223-013-0284-0

  • Du, J., Chadalavada, S., Chen, Z., & Naidu, R. (2014). Environmental remediation techniques of tributyltin contamination in soil and water: a review. Chemical Engineering Journal, 235, 141–150. doi:10.1016/j.cej.2013.09.044.

    Article  CAS  Google Scholar 

  • Dubey, S. K., Tokashiki, T., & Suzuki, S. (2006). Microarray-mediated transcriptome analysis of the tributyltin (TBT)-resistant bacterium Pseudomonas aeruginosa 25W in the presence of TBT. Journal of Microbiology, 44(2), 200–205.

    CAS  Google Scholar 

  • Fukushima, K., Dubey, S. K., & Suzuki, S. (2009). Quantitative analysis of expression of tributyltin (TBT)-regulated genes in TBT-resistant Pseudomonas aeruginosa 25W. Environmental Research in Asia, 163–166. https://www.terrapub.co.jp/onlineproceedings/ec/02/pdf/ERA18.pdf. Accessed 8 March 2016.

  • Gadd, G. M. (2000). Microbial interactions with tributyltin compounds: detoxification, accumulation, and environmental fate. Science of the Total Environment, 258(1–2), 119–127. doi:10.1016/S0048-9697(00)00512-X.

  • Gianguzza, A. (1997). Organotin—environmental fate and effects. Marine Chemistry (Vol. 57). Springer Science & Business Media. doi:10.1016/S0304-4203(97)88167-4

  • Gokulakrishnan, S., & Gummadi, S. N. (2006). Kinetics of cell growth and caffeine utilization by Pseudomonas sp. GSC 1182. Process Biochemistry, 41(6), 1417–1421. doi:10.1016/j.procbio.2005.12.018.

    Article  CAS  Google Scholar 

  • Harino, H., Arai, T., Ohji, M., Ismail, A. B., & Miyazaki, N. (2008). Organotin contaminations in Malaysia. Coastal Marine Science, 32(1), 96–101.

    Google Scholar 

  • Jude, F., Capdepuy, M., Quentin, C., Lascourrèges, J. F., & Caumette, P. (1996, June). Evaluation of tributyltin resistance in marine sediment bacteria. Canadian Journal of Microbiology. doi:10.1139/m96-071

  • Khanolkar, D. S., Dubey, S. K., & Naik, M. M. (2015). Biotransformation of tributyltin chloride to less toxic dibutyltin dichloride and monobutyltin trichloride by Klebsiella pneumoniae strain SD9. International Biodeterioration and Biodegradation, 104, 212–218. doi:10.1016/j.ibiod.2015.04.030.

    Article  CAS  Google Scholar 

  • Khanolkar, DS, Naik, MM, Dubey, SK. (2014). Biotransformation of Tributyltin chloride by Pseudomonas stutzeri strain DN2. Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 1245(4), 1239–1245. doi:http://dx.doi.org/10.1590/S1517-83822014000400014

  • Mohamat-Yusuff, F, Ahmad, I, Harino, H, Emi, Y, Selvahganesh, P, Zulkifli, S. (2013). Concentration of organotin compounds and the correlation with physicochemical parameter in core sediment from two different scale piers at the Strait of Johore. In NRCT-JSPS Joint International Seminar on Coaltal Marine Science in Southeast Asia (p. 87). Chiang Mai, Thailand

  • Mohamat-Yusuff, F., Zulkifli, S. Z., Ismail, A., Harino, H., Yusoff, M. K., & Arai, T. (2010). Imposex in Thais gradata as a biomarker for TBT contamination on the Southern Coast of Peninsular Malaysia. Water, Air, and Soil Pollution, 211(1-4), 443–457. doi:10.1007/s11270-009-0314-3.

    Article  CAS  Google Scholar 

  • Roy, U., Dubey, S. K., & Bhosle, S. (2004). Tributyltin chloride-utilizing bacteria from marine ecosystem of west coast of India. Current Science, 86(5), 702–705. http://www.scopus.com/scopus/inward/record.url?eid=2-s2.0-4444270935&partnerID=40.

    Google Scholar 

  • Roy, U., & Nair, D. (2007). Biodiversity of organotin resistant Pseudomonas from west coast of India. Ecotoxicology, 16(2), 253–261. doi:10.1007/s10646-006-0125-x.

    Article  Google Scholar 

  • Rüdel, H. (2003). Case study: bioavailability of tin and tin compounds. Ecotoxicology and Environmental Safety, 56(1), 180–189. doi:10.1016/S0147-6513(03)00061-7.

    Article  Google Scholar 

  • Sakultantimetha, A., Keenan, H. E., Beattie, T. K., Bangkedphol, S., & Cavoura, O. (2011). Bioremediation of tributyltin contaminated sediment: degradation enhancement and improvement of bioavailability to promote treatment processes. Chemosphere, 83(5), 680–686. doi:10.1016/j.chemosphere.2011.02.024.

    Article  CAS  Google Scholar 

  • Santillo, D., & Langston, W. J. (2001). Tributyltin (TBT) antifoulants: a tale of ships, snails and imposex. Late lessons from early warnings: the precautionary principle, 1896–2000(22), 135–148. http://reports.eea.eu.int/environmental_issue_report_2001_22/en/tab_abstract_RLR.

    Google Scholar 

  • Sudaryanto, A., Takahashi, S., Iwata, H., Tanabe, S., & Ismail, A. (2004). Contamination of butyltin compounds in Malaysian marine environments. Environmental Pollution, 130(3), 347–358. doi:10.1016/j.envpol.2004.01.002.

  • Terlizzi, A., Delos, A. L., Garaventa, F., Faimali, M., & Geraci, S. (2004). Limited effectiveness of marine protected areas: imposex in Hexaplex trunculus (Gastropoda, Muricidae) populations from Italian marine reserves. Marine Pollution Bulletin, 48(1-2), 188–92. doi:10.1016/j.marpolbul.2003.10.019.

    Article  CAS  Google Scholar 

  • Thoonen, S. H., Deelman, B.-J., & van Koten, G. (2004). Synthetic aspects of tetraorganotins and organotin(IV) halides. Journal of Organometallic Chemistry, 689(13), 2145–2157. doi:10.1016/j.jorganchem.2004.03.027.

    Article  CAS  Google Scholar 

  • Wuertz, S., Miller, C. E., Pfister, R. M., & Cooney, J. J. (1991). Tributyltin-resistant bacteria from estuarine and freshwater sediments. Applied and Environmental Microbiology, 57(10), 2783–2789. http://aem.asm.org/content/57/10/2783.short.

    CAS  Google Scholar 

Download references

Acknowledgments

Praise to God for the blessings bestowed upon us. Thanks to Dr. Auwal Suleiman of the Kaduna State Ministry of Health, Nigeria and Barr. Shamsuddeen Magaji of the Bauchi State University Gadau, Nigeria, for proofreading the article. This research was funded by FRGS/1/2014/STWN01/UPM/02/4.

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Authors and Affiliations

  1. Department of Environmental Sciences, Faculty of Environmental Studies, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    Abdussamad Abubakar, Wan Lutfi Wan Johari & Ferdaus Binti Mohamat Yusuff

  2. Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    Muskhazli B Mustafa & Syaizwan Zahmir Zulkifli

  3. Environmental Forensics Research Centre ENFORCE, Fakulti of Environmental Studies, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    Abdussamad Abubakar, Wan Lutfi Wan Johari & Ferdaus Binti Mohamat Yusuff

  4. Microbiology Department, Faculty of Science, Bauchi State University, Gadau, PMB 65, Nigeria

    Abdussamad Abubakar

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  1. Abdussamad Abubakar
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  2. Muskhazli B Mustafa
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  3. Wan Lutfi Wan Johari
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  4. Syaizwan Zahmir Zulkifli
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  5. Ferdaus Binti Mohamat Yusuff
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Correspondence to Ferdaus Binti Mohamat Yusuff.

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Highlights

This work was a continuation of previous study titled “Klebsiella sp. FIRD 2, a TBT-resistant bacterium isolated from contaminated surface sediment along the Strait of Johor Malaysia”.

The study is anticipated to make significant impact in conducting future research on TBT bioremediation in the tropical areas.

TBT tolerances of indigenous and non-indigenous bacterial species were successfully assessed, and the study ultimately endorses the potentials of indigenous bacteria in bioremediation of TBT contamination.

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Abubakar, A., Mustafa, M.B., Johari, W.L.W. et al. Tributyltin (TBT) Tolerance of Indigenous and Non-indigenous Bacterial Species. Water Air Soil Pollut 227, 258 (2016). https://doi.org/10.1007/s11270-016-2946-4

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  • DOI: https://doi.org/10.1007/s11270-016-2946-4

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