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Antibiotic Resistance and intI1 Carriage in Waterborne Enterobacteriaceae

  • Anca FarkasEmail author
  • Brînduşa Bocoş
  • Anca Butiuc-Keul
Article

Abstract

The spread of antimicrobial resistance (AMR) is a global concern, high research priority being given to the environmental contamination, as the prevalence of organisms exhibiting AMR continues to increase. Multiresistant bacteria carrying different mobile genetic elements have been detected in sites with different degrees of urbanization, surface waters receiving insufficiently treated effluents being at high risk. The aim of the present study was to investigate the loads, antibiotic susceptibility, and class 1 integron carriage of Enterobacteriaceae isolated from surface waters and wastewaters around a large Romanian city. Searching for a valuable genetic marker of the displayed antibiotic resistance, the link between the AMR and the presence of int1I gene was explored in a total of 166 waterborne strains. Overall, amoxicillin-clavulanate resistance displayed the highest frequency (71.1 %), followed by ampicillin (63.9 %), cefuroxime (21.1 %), ciprofloxacin (17.5 %), cefotaxime (15.7 %), ceftriaxone (10.8 %), and gentamicin (6.6 %). The frequencies of isolates resistant to ampicillin, amoxicillin-clavulanate, ciprofloxacin, and gentamicin and also the prevalence of multiresistant strains were greater in surface waters, compared to wastewaters. The Int1I gene was detected in 21.7 % waterborne Enterobacteriaceae. A decrease in coliform counts and intI1-bearing cells, but a general increase in AMR and multiresistant bacteria, occurred during the wastewater treatment. A weak positive correlation was found between multidrug resistance int1I carriage in wastewater effluent but no sufficient evidence of a linkage between phenotypic AMR and int1I, overall. The presence of class 1 integron can be associated with anthropogenic influence, but the simple detection of intI1 gene cannot explain the complex antibiotic resistance phenotype.

Keywords

Antibiotic resistance intI1 Aquatic environment Wastewater Enterobacteriaceae 

Notes

Acknowledgments

This work has been partially supported by the grant RO-04 66230, Methodological guide for monitoring antibiotic residues and antibiotic resistance in the environment as a supporting instrument for an enhanced quality management of surface waters and groundwater (EnviroAMR), financed by Iceland, Liechtenstain and Norway through the EEA Financial Mechanism.

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Anca Farkas
    • 1
    Email author
  • Brînduşa Bocoş
    • 2
  • Anca Butiuc-Keul
    • 1
  1. 1.Department of Molecular Biology and BiotechnologyBabeş-Bolyai UniversityCluj-NapocaRomania
  2. 2.National Public Health Institute–Regional Public Health Centre of ClujCluj-NapocaRomania

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