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DNA Damage and Oxidative Stress in Marine Gastropod Morula granulata Exposed to Phenanthrene

Abstract

DNA damage and oxidative stress in marine gastropod Morula granulata was measured after in vivo exposure to four different concentrations (10, 25, 50, and 100 μg/L) of phenanthrene. Comet assay was used for measurement of DNA damage, whereas oxidative stress was assessed using a battery of biomarkers such as glutathione-S-transferase (GST), catalase (CAT), and lipid peroxidation (LPO). Our data showed concentration-dependent increase in percentage DNA in tail (TDNA), LPO, and GST activity in gastropods exposed to phenanthrene. CAT activity in gastropods was not found to be consistent with the phenanthrene concentrations. Significant increase in TDNA was observed at all concentrations above 10 μg/L of phenanthrene. Positive correlations were observed among oxidative stress biomarker and TDNA. Integrated biomarker response (IBR) analysis showed that among the four biomarkers, LPO and DNA damage (TDNA) were the most sensitive in response to phenanthrene exposure. Our results clearly showed that phenanthrene is genotoxic to gastropods and also causes oxidative stress.

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Acknowledgments

The financial support by Department of Biotechnology, New Delhi, in the form of Senior Research Fellowship (SRF) to Jacky Bhagat is highly acknowledged. The authors also would like to thank the Director of CSIR-NIO for his hearted cooperation. This research was financially supported by Council of Scientific and Industrial Research (CSIR) through Project No. PSC0206. This is contribution no……….. of CSIR, NIO, Goa.

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Correspondence to Jacky Bhagat.

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Highlights

• An approach to evaluate genotoxicity of phenanthrene in marine gastropod was presented

• In vivo effects of phenanthrene in gastropod was evaluated using genotoxic and oxidative stress biomarker

• Multi-biomarker approach was assessed in marine gastropod

• Phenanthrene was found to be genotoxic to marine gastropod Morula granulata

• Positive correlations were observed in GST, LPO, and % DNA in tail in snails exposed to phenantherene

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Fig. S1

Sampling site at Arambol, Goa, India (JPG 216 kb)

Fig. S2

Morula granulata spread over the oyster belt in the intertidal rocks scattered along Arambol, Goa, India (JPG 3087 kb)

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Bhagat, J., Sarkar, A. & Ingole, B.S. DNA Damage and Oxidative Stress in Marine Gastropod Morula granulata Exposed to Phenanthrene. Water Air Soil Pollut 227, 114 (2016). https://doi.org/10.1007/s11270-016-2815-1

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  • DOI: https://doi.org/10.1007/s11270-016-2815-1

Keywords

  • Gastropods
  • Genotoxicity
  • Biomarker
  • Antioxidant enzyme
  • Comet assay