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Effects of Carbamazepine on Two Microalgae Species Differing in Stress Resistance

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Abstract

Carbamazepine (CBZ) is a representative of a group of compounds found in our rivers that have been classified as upcoming contaminants. Its pharmacological activity to treat mood and neurological disorders is based on its effects on ion channels, but effects on aquatic organisms have not yet been thoroughly investigated.

In our initial analysis, we compared CBZ effects on two microalgae species differing in CBZ sensitivity: Parachlorella kessleri and Neochloris pseudoalveolaris. While we observed a stimulation in the growth rate in cultures of P. kessleri in the presence of 10 μg L−1 CBZ, no effect on growth rates of N. pseudoalveolaris cultures could be documented at this concentration. Any higher tested CBZ concentration led to growth inhibition.

To gain insight into these effects, biochemical and physiological parameters of these two microalgae species were measured in the presence of CBZ in a concentration-dependent manner.

As the severe inhibition of growth rate correlated with a significant inhibition of most tested parameters in cultures of N. pseudoalveolaris, the primary reason for the adverse effect of CBZ on cultures of this microalgae species could not be identified. In cultures of N. pseudoalveolaris, experimental data indicate that inhibition of growth rate occurs when the microalgae are no longer able to compensate for adverse CBZ-induced ROS effects.

Analysis of the CBZ response of cultures of P. kessleri showed a reduction of growth stimulatory effect if the CBZ concentration exceeds a threshold value. In general, cultures of P. kessleri show a great potential to withstand CBZ as an environmental pollutant.

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Acknowledgements

We thank Silke Braams, Leibniz Universität Hannover, for providing data on lipid peroxidation and Yelena Churakova, Northeastern University, Boston, for proof-reading.

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Authors and Affiliations

  1. Institute of Horticultural Production Systems, Biosystems Engineering, Leibniz Universität Hannover, Herrenhäuser Str. 2, 30419, Hannover, Germany

    Sandra M. Haase & Paweł Panas

  2. Institute of Botany, Leibniz Universität Hannover, Herrenhäuser Str. 2, 30419, Hannover, Germany

    Sandra M. Haase, Paweł Panas & Bernhard Huchzermeyer

  3. Biosystems Engineering Laboratory, Hochschule Osnabrück, Oldenburger Landstraße 24, 49090, Osnabrück, Germany

    Thomas Rath

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  1. Sandra M. Haase
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  2. Paweł Panas
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Correspondence to Sandra M. Haase.

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Haase, S.M., Panas, P., Rath, T. et al. Effects of Carbamazepine on Two Microalgae Species Differing in Stress Resistance. Water Air Soil Pollut 226, 328 (2015). https://doi.org/10.1007/s11270-015-2562-8

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