Production of Volatile Fatty Acids during the Hydrolysis and Acidogenesis of Pistia stratiotes Using Ruminal Fluid
- 549 Downloads
Aquatic plant biomass has been shown to have a great potential for biogas production. The use of ruminal fluid has been shown to improve the degradation of the lignocellulosic material with its conversion into volatile fatty acids (VFA) during a first phase of hydrolysis–acidogenesis. VFAs are important as the feedstock for methane and hydrogen production in a second phase process within a biorefinery. The objective of this work was to produce a high yield of VFA during a first phase of anaerobic hydrolysis–acidogenesis of Pistia stratiotes biomass assessing the effect of the use of rumen fluid as inoculum and of daily adjustment of pH in batch-operated reactors. One liter anaerobic reactors containing 15 gSV L−1 of P. stratiotes biomass were incubated at 30 ± 2 °C and agitated once a day. The inoculum concentration had no significant effect on the increase in VFA concentration and 20 % (V/V) was used in all treatments. The final average VFA concentration and conversion coefficients from VS to VFA in the inoculated treatment with no pH adjustment (T1) and with pH adjustment (T2) (1817 mgCOD L−1 and 0.1319 mgVFA mgVS−1, respectively) were significantly higher than those found in the treatment with no inoculum (T0). There were no significant differences between T0 and T1 in the VS degradation rate. In contrast, the degradation rate in T2 was significantly higher. Thus, the addition of ruminal fluid promoted the production of VFA, and the pH adjustment had no significant effect on this parameter but did influence the biomass degradation.
KeywordsAnaerobic digestion Lignocellulosic material Floating macrophyte
This study was funded by the Ministry of Energy (SENER) and the National Council of Science and Technology Mexico (CONACYT) through the grant 152931. Héctor Hernández-García received a scholarship (409049/261224) for graduate studies from CONACYT.
Compliance with Ethical Standards
This research did not involve human participants and/or animals.
Conflict of Interest
The authors declare that they have no competing interests.
- ANKOM Technology. (2011a). Acid detergent fiber in feeds filter bags techniques. Method 5.Google Scholar
- ANKOM Technology. (2011b). Neutral detergent fiber in feeds filter bags techniques. Method 6.Google Scholar
- ANKOM Technology. (2011c). Acid detergent lignin in beakers. Method 8.Google Scholar
- APHA, AWWA, WPCF. (1998). Standard Methods for the Examination of Water and Wastewater. 20th Ed. NY, USA.Google Scholar
- Awuah, E., Anohene, F., Asante, K., Lubberding, H., & Gijzen, H. (2001). Environmental conditions and pathogen removal in macrophyte- and algal-based domestic wastewater treatment systems. Water Science and Technology, 44(6), 11–18.Google Scholar
- Barnes, S. P., & Keller, J. (2004). Anaerobic rumen SBR for degradation of cellulosic material. Water Science and Technology, 50(10), 305–311.Google Scholar
- Benerjee, A., & Matai, S. (1990). Composition of Indian aquatic plants in relation to utilization as animal forage. Journal of Aquatic Plant Management, 28(15), 69–73.Google Scholar
- Carranco, M. E., Castillo, R. M., Escamilla, A., Martínez, M., Pérez-Gil, F., & Stephan, E. (2002). Composición química, extracción de proteína foliar y perfil de aminoácidos de siete plantas acuáticas. Revista Cubana de Ciencia Agrícola, 36(3), 247–258.Google Scholar
- Contreras, P. A., & Noro, M. (2010). Rumen: morfología, trastornos y modulación de la actividad fermentativa. Valdivia: América.Google Scholar
- Cysneiros, D., Banks, C. J., Heaven, S., & Karatzas K-A, G. (2012). The role of phase separation and feed cycle length in leach beds coupled to methanogenic reactors for digestion of a solid substrate (part 1): optimisation of reactors’ performance. Bioresource Technology, 103(1), 56–63.CrossRefGoogle Scholar
- HACH. (2000a). Chemical oxygen demand, Reactor digestion method. Method 8000.Google Scholar
- HACH. (2000b). Volatile acids, Esterification method. Method 8196.Google Scholar
- Henry-Silva, G. G., & Camargo, A. F. M. (2002). Valor nutritivo de macrófitas aquáticas flutuantes (Eichhornia crassipes, Pistia stratiotes e Salvinia molesta) utilizadas no tratamento de efluentes de aqüicultura. Maringá: Acta Scientiarum, 24(37), 519–526.Google Scholar
- Lehtomäki, A. (2006). Biogas production from energy crops and crop residues. Jyväskylä: University of Jyväskylä.Google Scholar
- Olguín, E. J., Sánchez-Galván, G., Pérez-Pérez, T., & Pérez-Orozco, A. (2005). Surface adsorption, intracellular acumulation and compartmentalization of Pb(II) in batch-operated lagoons with Salvinia minima as affected by environmental conditions, EDTA and nutrients. Journal Industrial Microbiology & Biotechnology, 32(11–12), 577–586.CrossRefGoogle Scholar
- Olguín, E. J., González-Portela, R. E., Sánchez-Galván, G., Zamora-Castro, J. E., & Owen, T. (2010). Contaminación de ríos urbanos: El caso de la subcuenca del Río Sordo en Xalapa, Veracruz, México. Revista Latinoamericana de Biotecnología Ambiental y Algal, 1(2), 178–190.Google Scholar
- Rodríguez, R., Julio, C., & Palma, J. (2000). Valor nutritivo del repollito de agua (Pistia stratiotes L.) y su posible uso en la alimentación animal. Zootecnia Tropical, 18(2), 213–226.Google Scholar
- Yue Z.B., Yu H.Q., Harada H., Li Y.Y., (2007a). Optimization of anaerobic acidogenesis of an aquatic plant, Canna indica L., by rumen cultures. Water Research, 41(11), 2361–2370Google Scholar
- Zennaki, Z., Zaid, A., Bentaya, K., & Boulif, M. (1997). Optimization of anaerobic digestion of cattle manure effect of its association with the aquatic weed pistia (Pistia stratiotes). Tropicultura, 15(2), 51–55.Google Scholar