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Biodegradation of Fats and Oils in Domestic Wastewater by Selected Protozoan Isolates

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Abstract

The majority of the existing water bodies around the world are increasingly polluted with oily wastewater. The aim of this study was to investigate the role of single protozoan isolates (Aspidisca, Trachelophyllum and Peranema) and of a consortium of these three protozoan isolates in the biodegradation of fats and oils present in polluted domestic wastewater. The biomass of protozoan isolates, chemical oxygen demand (COD), dissolved oxygen (DO) and concentrations of fats and oils were determined in triplicate before and after the inoculation of isolates in oily wastewaters, using standard methods. Results revealed optimum growth of protozoan cell densities under favourable conditions of 30 °C, pH 6 and 8 (from 1.00 to 4.00, 3.96, 3.80 and 4.20 × 102 cells/ml for Aspidisca, Trachelophyllum, Peranema and a consortium of the three isolates, respectively). The average percentage uptake of DO by Aspidisca, Trachelophyllum, Peranema and their consortium was 95, 96, 96 and 100 %, respectively, for both 30 and 25 °C and at pH levels of (4, 6, 8 and 10), respectively. The results revealed that the COD removal rates of the isolates at various pH levels were ≥20 and ≤90 %, respectively, for 30 and 25 °C. At a temperature of 30 °C, the biodegradation capabilities of the isolates ranged from 3.0 to 8.0, 3.0 to 6.0, 7.0 to 11.0 and 8.0 to 22.0 %, while at 25 °C, the biodegradation rates were 3.0 to 6.0, 4.0 to 7.0, 3.0 to 8.0 and 4.0 to 15.0 % for Aspidisca, Trachelophyllum, Peranema and the consortium of these three isolates, respectively.

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Acknowledgments

The authors are grateful to the National Research Foundation (NRF) and Tshwane University of Technology (TUT) for their financial support.

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  1. Department of Environmental, Water and Earth Sciences, Faculty of Science, Tshwane University of Technology, Arcadia Campus, P/Bag X680, Pretoria, 0001, South Africa

    L. O. Kachieng’a & M. N. B. Momba

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  1. L. O. Kachieng’a
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  2. M. N. B. Momba
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Correspondence to M. N. B. Momba.

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Kachieng’a, L.O., Momba, M.N.B. Biodegradation of Fats and Oils in Domestic Wastewater by Selected Protozoan Isolates. Water Air Soil Pollut 226, 140 (2015). https://doi.org/10.1007/s11270-015-2405-7

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