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Evaluation of Mutagenic and Genotoxic Activity in Vinasses Subjected to Different Treatments

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Abstract

The mutagenic and genotoxic activity of vinasses collected from a fuel alcohol plant, located in the municipality of Frontino, Northwestern Colombia, were evaluated. Two samples obtained from an 82-L capacity hybrid reactor (UASB-anaerobic filter (AF)-UASB) were studied under laboratory conditions after being treated with biological oxidation, the first, and the second with Fenton reaction consecutively. Mutagenicity was evaluated in vitro by the Ames test using strains TA98 and TA100 with and without S9 metabolic activation. The genotoxic analysis was conducted using the Allium cepa roots assay where chromosomal aberrations were used as clastogenic or aneugenic response markers, and micronuclei as mutagenic response. The Ames test results showed a strain-dependent positive linear association with the vinasse sample concentration before treatment (dose–response effect). Unlike TA100, strain TA98 showed a mutagenic effect in both the presence and absence of metabolic enzymes. After the biological oxidation treatment, vinasse mutagenicity significantly decreased. Finally, after Fenton treatment, the sample did not induce any mutagenic event. Genotoxic activity was observed in all three samples, but there was a higher frequency in the vinasse sample before treatment. Concerning the frequency of micronuclei, no clear association was observed with either the concentration or the type of sample.

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Acknowledgments

We would like to thank the Ministry of Agriculture of Colombia and the Strategy of Groups Sustenance 2011–2012 of the University of Antioquia for financial support of this research.

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Authors and Affiliations

  1. Interdisciplinary Group of Molecular Studies (GIEM), University of Antioquia, P.O. Box 1226, Medellin, Colombia

    José Oñate, Armando Arenas, Alejandro Ruiz, Katherin Rivera & Carlos Peláez

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  1. José Oñate
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  2. Armando Arenas
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  3. Alejandro Ruiz
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  5. Carlos Peláez
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Correspondence to José Oñate.

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Oñate, J., Arenas, A., Ruiz, A. et al. Evaluation of Mutagenic and Genotoxic Activity in Vinasses Subjected to Different Treatments. Water Air Soil Pollut 226, 144 (2015). https://doi.org/10.1007/s11270-014-2250-0

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