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Influence of the Anaerobic Biodegradation of Different Types of Biodiesel on the Natural Attenuation of Benzene

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Abstract

In the present research work, different types of biodiesel were produced by a homogeneous alkali transesterification reaction using soybean oil, pork lard, and castor bean oil as raw materials, to evaluate how their different compositions may affect the biodegradability, namely, in the presence of benzene. Biodiesel was characterized according to the European standard EN 14214. The anaerobic biodegradation of the different types of biodiesel was examined as well as its influence on the biodegradation of benzene. Analyses were performed to determine the volume of methane (directly related to the anaerobic biodegradation of biodiesel), the concentration of benzene over time, and the production of organic acids. The results showed methane production resulting from the anaerobic degradation of all biodiesel types. The differences between the degradation behavior of each fuel were negligible, contrary to what was expected; however, the amount of methane produced was low due to nutrient limitations. This fact was confirmed by the organic acid analysis as well as by the addition of new media. Anaerobic benzene biodegradation was found to be negatively impacted by the presence of all biodiesel types on average; therefore, the results of this study may impact management of sites that contain biodiesel and fuel hydrocarbon contamination.

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Acknowledgments

The authors wish to thank the Portuguese Science and Technology Foundation (FCT) for the research grant SFRH/BPD/73809/2010, the Ciência 2008 Program, and other funds within the framework of the project PTDC/AAG-TEC/4403/2012, supported by FEDER funds through the Operational Program for Competitiveness Factors—COMPETE. The assistance of Professor Cristina Vila and Professor Olga Nunes (FEUP) for various aspects of this work is greatly appreciated.

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Authors and Affiliations

  1. Geo-environmental and Resources Research Center (CIGAR), Department of Mining Engineering, Faculty of Engineering of Oporto University (FEUP), Rua Dr. Roberto Frias s/n, 4200-465, Oporto, Portugal

    J. M. Borges & A. S. Danko

  2. Laboratory for Process Engineering, Environment, Biotechnology and Energy (LEPABE), Department of Metallurgical and Materials Engineering, Faculty of Engineering of Oporto University (FEUP), Rua Dr. Roberto Frias s/n, 4200-465, Oporto, Portugal

    J. M. Borges & J. M. Dias

  3. Centre for Natural Resources and the Environment (CERENA), Instituto Superior Técnico, Av. Rovisco Pais, 1049-001, Lisbon, Portugal

    A. S. Danko

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  1. J. M. Borges
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  2. J. M. Dias
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Correspondence to A. S. Danko.

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Borges, J.M., Dias, J.M. & Danko, A.S. Influence of the Anaerobic Biodegradation of Different Types of Biodiesel on the Natural Attenuation of Benzene. Water Air Soil Pollut 225, 2146 (2014). https://doi.org/10.1007/s11270-014-2146-z

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