Effects of Perchlorate Stress on Growth and Physiological Characteristics of Rice (Oryza sativa L.) Seedlings
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Effects of perchlorate stress on the growth and physiological characteristics of rice (Oryza sativa L.) were studied in controlled water culture experiments. Perchlorate stress resulted in varied patterns of biomass allocation to O. sativa organs (roots, stems, and leaves). When stressed with higher perchlorate concentrations, the proportion of root biomass was higher, stem biomass was invariant, while leaf biomass was lower. Coefficients of variation in biomass of different organs followed the order leaf > stem > root, indicating that leaf growth has a higher sensitivity to perchlorate. Compared to the control, the chlorophyll and protein content of leaves and root vigor were lower, whereas malondialdehyde (MDA) content and catalase (CAT) activity were higher and related to perchlorate concentration and duration of stress. Superoxide dismutase (SOD) activity was initially high and then decreased markedly during the experiment, while peroxidase (POX) activity in perchlorate-treated rice was always higher than the POX activity of the control. POX was the most sensitive antioxidant enzyme to perchlorate stress. Correlation analysis showed a positive correlation between SOD activity and the fresh weight of the whole plant, and negative correlation with MDA content. The results suggest that perchlorate could induce oxidative stress and oxidative damage may be the main cause of physiological damage and growth inhibition in rice plants under perchlorate stress.
KeywordsPerchlorate stress Growth Physiology Oryza sativa L
The present study was funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), National Basic Research Program of China (2006CB403301), and Scientific Innovation Fund of Nanjing Forestry University (CX2011-23). We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.
- Bai, B. Z., Jin, J. Z., & Bai, S. (1994). Improvement of TTC method determining root activity in corn. Journal of Maize Sciences, 2, 44–47 (in Chinese).Google Scholar
- Cai, X. L., Xie, Y. F., & Liu, W. L. (2008). An overview of researches on environmental contamination by perchlorate and its remediation. Acta Ecologica Sinica, 28, 5592–5600 (in Chinese).Google Scholar
- Dowling, A. L., Martz, G. U., Leonard, J. L., & Zoeller, R. T. (2000). Acute changes in maternal thyroid hormone induce rapid and transient changes in gene expression in fetal rat brain. The Journal of Neuroscience, 20, 2255–2265.Google Scholar
- Li, H. S. (2000). The experiment principles and techniques of plant physiology and biochemistry. Beijing: Higher Education Press.Google Scholar
- Li, L. (2009). Modular experimental guiding of plant physiology. Beijing: Science Press.Google Scholar
- Mao, D. R. (2001). Research methods of plant nutrition. Beijing: China Agricultural University Press.Google Scholar
- Radovic, S., Vidakovic, C. Z., Tkalec, M., Krsnik, R. M., & Regula, I. (2005). Peroxidase and proteins as salinity stress indicators in duckweed exposed to oil industry high density brines. Periodicum Biologorum, 107, 33–38.Google Scholar
- Sun, C. H., Du, W., Cheng, X. L., Xu, X. N., Zhang, Y. H., Sun, D., et al. (2010). The effects of drought stress on the activity of acid phosphatase and its protective enzymes in pigweed leaves. African Journal of Biotechnology, 9, 825–833.Google Scholar
- Xie, Y. F., Cai, X. L., & Liu, W. L. (2009). Effects of perchlorate on growth and chlorophyll fluorescence parameters of Alternanthera philoxeroides. Environmental Science, 30, 2425–2431 (in Chinese).Google Scholar