Skip to main content
SpringerLink
Log in

Effect of Zn2+ on the Performances and Methanogenic Community Shifts of UASB Reactor During the Treatment of Swine Wastewater

  • Published:
Water, Air, & Soil Pollution Aims and scope Submit manuscript

Abstract

Zn2+ was added to one of the two identical bench-scale upflow anaerobic sludge blanket (UASB) reactors in steady-state period treating swine wastewater to evaluate the effects of Zn2+ on performance and methanogenic population. Real-time quantitative PCR (QPCR) was used to quantify the 16S rRNA gene concentrations of the four methanogenic orders. In both reactors, the hydraulic retention time (HRT) was sustained at 48 h and the inner temperature was kept at 35 °C. Both promotion and inhibition of Zn2+ on chemical oxygen demand (COD) removal, methane production and methanogens community were observed in accordance with different Zn2+ dosages. COD removal rate and methane production reacted in the same way as methanogens, suggesting that the impact of Zn2+ on the methanogenic community was the critical reason that caused the changes of UASB performance in treating swine wastewater with unstable Zn2+ concentration. Among the methanogenic community, Methanomicrobiales (MMB) was the dominant group which got visibly impacted by the dosed Zn2+. Overall, lower concentration of Zn2+, e.g., less than 17.8 mg/L, was supposed to be advisable for a stable and high efficient treatment of swine wastewater by UASB reactor in practice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Akarsubasi, A. T., Ince, O., Kirdar, B., Oz, N. A., Orhon, D., Curtis, T. P., et al. (2005). Effect of wastewater composition on archaeal population diversity. Water Research, 39(8), 1576–1584.

    Article  CAS  Google Scholar 

  • Angenent, L. T., Sung, S., & Raskin, L. (2002). Methanogenic population dynamics during startup of a full-scale anaerobic sequencing batch reactor treating swine waste. Water Research, 36(18), 4648–4654.

    Article  CAS  Google Scholar 

  • APHA. (2005). Standard methods for the examination on of water and wastewater (18th ed.). Washington D.C: American Public Health Association.

    Google Scholar 

  • Boone, D. R., & Castenholz, R. W. (Eds.). (2001). Bergey’s manual of systematic bacteriology: the archaea and the deeply branching and phototrophic bacteria, second ed (Vol. 1). New York: Springer.

    Google Scholar 

  • Case, C. L., & Carlson, M. S. (2002). Effect of feeding organic and inorganic sources of additional zinc on growth performance and zinc balance in nursery pig. Journal of Animal Science, 80(7), 1917–1924.

    CAS  Google Scholar 

  • Chen, Y., Cheng, J. J., & Creamer, K. S. (2008). Inhibition of anaerobic digestion process: a review. Bioresource Technology, 99(10), 4044–4064.

    Article  CAS  Google Scholar 

  • Cho, K., Lee, J., Kim, W., & Hwang, S. (2013). Behavior of methanogens during start-up of farm-scale anaerobic digester treating swine wastewater. Process Biochemistry, 48(9), 1441–1445.

    Article  CAS  Google Scholar 

  • Cook, K. L., Rothrock, M. J., Jr., Loughrin, J. H., & Doerner, K. C. (2007). Characterization of skatole-producing microbial populations in enriched swine lagoon slurry. FEMS Microbiology Ecology, 60(2), 329–340.

    Article  CAS  Google Scholar 

  • Fermoso, F. G., Collins, G., Bartacek, J., & Lens, P. (2008a). Zinc deprivation of methanol fed anaerobic granular sludge bioreactors. Journal of Industrial Microbiology and Biotechnology, 35(6), 543–557.

    Article  CAS  Google Scholar 

  • Fermoso, F. G., Collins, G., Bartacek, J., O’Flaherty, V., & Lens, P. (2008b). Acidification of methanol-fed anaerobic granular sludge bioreactors by cobalt deprivation: induction and microbial community dynamics. Biotechnology and Bioengineering, 99(1), 49–58.

    Article  CAS  Google Scholar 

  • Fermoso, F. G., Bartacek, J., Jansen, S., & Lens, P. (2009). Metal supplementation to UASB bioreactors: from cell-metal interactions to full-scale application. Science of the Total Environment, 407(12), 3652–3667.

    Article  CAS  Google Scholar 

  • Huang, J. S., Wu, C. S., & Chen, C. M. (2005). Microbial activity in a combined UASB-activated sludge reactor system. Chemosphere, 61(7), 1032–1041.

    Article  CAS  Google Scholar 

  • Jacela, J. Y., DeRouchey, J. M., & Tokach, M. D. (2010). Feed additives for swine: fact sheets-high dietary levels of copper and zinc for young pigs, and phytase. Journal of Swine Health and Production, 18(2), 87–91.

    Google Scholar 

  • Jiang, P., Jin, S. Y., Hao, X. Z., Zhou, D. M., & Li, L. Z. (2010). Distribution characteristics of heavy metals in feeds, pig manures, soils and vegetables. Journal of Agro-Environment Science, 29(5), 942–947.

    CAS  Google Scholar 

  • Kim, W., Lee, S., Shin, S. G., Lee, C., Hwang, K., & Hwang, S. (2010). Methanogenic community shift in anaerobic batch digesters treating swine wastewater. Water Research, 44(17), 4900–4907.

    Article  CAS  Google Scholar 

  • Lee, C., Kim, J., Hwang, K., O’Flaherty, V., & Hwang, S. (2009). Quantitative analysis of methanogenic community dynamics in three anaerobic batch digesters treating different wastewaters. Water Research, 43(1), 157–165.

    Article  CAS  Google Scholar 

  • MEPC (Ministry of Environmental Protection of China). (2011). Discharge standard of pollutants for livestock and poultry farms. GB 18596–2001. Beijing: Ministry of Environmental Protection of China.

    Google Scholar 

  • Mi, W. J., Cai, J. B., TUO, Y., Zhu, H., Hua, Y. M., Zhao, J. W., et al. (2013). Distinguishable root plaque on root surface of Potamogeton crispus grown in two sediments with different nutrient status. Limnology, 14(1), 1–11.

    Article  Google Scholar 

  • Najafpour, G. D., Zinatizadeh, A. A. L., & Mohamed, A. R. (2006). High-rate anaerobic digestion of palm oil mill effluent in an upflow anaerobic sludge-fixed film bioreactor. Process Biochemistry, 41(2), 370–379.

    Article  CAS  Google Scholar 

  • Ram, M. S., Singh, L., Suryanarayana, M. V. S., & Alam, S. I. (2000). Effect of iron, nickel and cobalt on bacterial activity and dynamics during anaerobic oxidation of organic matter. Water, Air, and Soil Pollution, 117(1–4), 305–312.

    Article  Google Scholar 

  • Sánchez, E., Borja, R., Travieso, L., & Martín, A. (2005). Effect of influent substrate concentration and hydraulic retention time on the performance of down-flow anaerobic fixed bed reactors treating piggery wastewater in a tropical climate. Process Biochemistry, 40(2), 817–3829.

    Article  Google Scholar 

  • Sawayama, S., Tada, C., Tsukahara, K., & Yagishita, T. (2004). Effect of ammonium addition on methanogenic community in a fluidized bed anaerobic digestion. Journal of Bioscience and Bioengineering, 97(1), 65–70.

    Article  CAS  Google Scholar 

  • Song, M., Shin, S. G., & Hwang, S. (2010). Methanogenic population dynamics assessed by real-time quantitative PCR in sludge granule in upflow anaerobic sludge blanket treating swine wastewater. Bioresource Technology, 101(1), 523–528.

    Article  Google Scholar 

  • Tiwari, M. K., Guha, S., Harendranath, C. S., & Tripathi, S. (2006). Influence of extrinsic factors on granulation in UASB reactor. Applied Microbiology and Biotechnology, 71(2), 145–154.

    Article  CAS  Google Scholar 

  • Vavilin, V. A. (2012). Estimating evolution of δ13CH4 during methanization of cellulosic waste based on stoichiometric chemical reactions, microbial dynamics and stable carbon isotope fractionation. Bioresource Technology, 110, 706–710.

    Article  CAS  Google Scholar 

  • Yang, G. F., Ni, W. F., Wu, K., Wang, H., Yang, B. E., Jia, X. Y., et al. (2013). The effect of Cu (II) stress on the activity, performance and recovery on the anaerobic ammonium-oxidizing (Anammox) process. Chemical Engineering Journal, 226(15), 39–45.

    Article  CAS  Google Scholar 

  • Yu, H. Q., & Fang, H. H. P. (2001). Inhibition on acidogenesis of dairy wasteby zinc and copper. Environmental Technology, 22(12), 1459–1465.

    Article  CAS  Google Scholar 

  • Yu, Y., Lee, C., Kim, J., & Hwang, S. (2005). Group-specific primer and probe sets to detect methanogenic communities using quantitative real-time polymerase chain reaction. Biotechnology and Bioengineering, 89(6), 670–679.

    Article  CAS  Google Scholar 

  • Yu, Y., Kim, J., & Hwang, S. (2006). Use of real-time PCR for group-specific quantification of aceticlastic methanogens in anaerobic processes: population dynamics and community structures. Biotechnology and Bioengineering, 93(3), 424–433.

    Article  CAS  Google Scholar 

  • Zayed, G., & Winter, J. (2000). Inhibition of methane production from whey by heavy metals-protective effect of sulfide. Applied Microbiology and Biotechnology, 53(6), 726–731.

    Article  CAS  Google Scholar 

  • Zhang, J. X., Zhang, Y. B., Quan, X., & Chen, S. (2013). Effects of ferric iron on the anaerobic treatment and microbial biodiversity in a coupled microbial electrolysis cell (MEC)–anaerobic reactor. Water Research. doi:10.1016/j.watres.2013.06.056.

    Google Scholar 

  • Zheng, D., Angenent, L. T., & Raskin, L. (2006). Monitoring granule formation in anaerobic upflow bioreactors using oligonucleotide hybridization probes. Biotechnology and Bioengineering, 94(3), 458–472.

    Article  CAS  Google Scholar 

  • Zhou, L. J., Ying, G. G., Liu, S., Zhang, R. Q., Lai, H. J., Chen, Z. F., et al. (2013). Excretion masses and environmental occurrence of antibiotics in typical swine and dairy cattle farms in China. Science of the Total Environment, 444(1), 183–195.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work is sponsored by National Science and Technology Major Project of the Ministry of Science and Technology of China (2012ZX07104-001) and the Fundamental Research Funds for the Central Universities (Program No.2011PY114).

Author information

Authors and Affiliations

  1. Laboratory of Plant Nutrition and Ecological Environment Research of Huazhong Agricultural University, Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, 430070, Wuhan, China

    Ye Tuo, Jianbo Cai, Duanwei Zhu, Ying Zhu, Guanglong Liu, Yumei Hua & Jiajie He

Authors
  1. Ye Tuo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jianbo Cai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Duanwei Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ying Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Guanglong Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yumei Hua
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jiajie He
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jianbo Cai.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tuo, Y., Cai, J., Zhu, D. et al. Effect of Zn2+ on the Performances and Methanogenic Community Shifts of UASB Reactor During the Treatment of Swine Wastewater. Water Air Soil Pollut 225, 1996 (2014). https://doi.org/10.1007/s11270-014-1996-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11270-014-1996-8

Keywords

Search

Navigation