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Sequential Microaerophilic-Oxic Phase Mineralization of Azo Dyes by a Monoculture of Pseudomonas Aeruginosa Strain AWF Isolated from Textile Wastewater

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Abstract

A novel dye degrading bacterium capable of decolorizing and mineralizing four different dyes (Methyl red, Orange II, Direct red 80, and Direct blue 71) was isolated from textile industrial wastewater using the selective enrichment technique. The bacterium was identified as Pseudomonas aeruginosa. More than 80 % decolorization of Direct red 80 was obtained under microaerophilic conditions in 48 h, whereas only 10 % color removal was obtained under oxic conditions at the same time. Subsequent aeration of the decolorized medium resulted in the mineralization of the metabolic intermediates generated after azo bond cleavage by P. aeruginosa as confirmed by total organic carbon content and high-performance liquid chromatography analyses. The degradation products were characterized by Fourier transform infrared spectrometer and nuclear magnetic resonance techniques whereas the biotoxicity profile of the samples were evaluated using the brine shrimp lethality test assay. Data from this study provide evidence of dye mineralization and detoxification by a monoculture of P. aeruginosa in successive microaerophilic/oxic stages.

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Acknowledgments

One of the authors (Ogugbue, C.J.) is thankful to the Academy of Science for the Developing World (TWAS) and the Universiti Sains Malaysia (USM) for awarding him the TWAS-USM Post Doctoral Fellowship.

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  1. Environmental Division, School of Industrial Technology, Universiti Sains Malaysia, Penang, Malaysia

    Maryam Khosravi Hafshejani, Chimezie Jason Ogugbue & Norhashimah Morad

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  1. Maryam Khosravi Hafshejani
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  2. Chimezie Jason Ogugbue
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  3. Norhashimah Morad
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Correspondence to Norhashimah Morad.

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Hafshejani, M.K., Ogugbue, C.J. & Morad, N. Sequential Microaerophilic-Oxic Phase Mineralization of Azo Dyes by a Monoculture of Pseudomonas Aeruginosa Strain AWF Isolated from Textile Wastewater. Water Air Soil Pollut 224, 1672 (2013). https://doi.org/10.1007/s11270-013-1672-4

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