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Effect of Disposal of Effluent and Paunch from a Meat Processing Factory on Soil Chemical and Microbial Properties

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Abstract

The effects of irrigation with meat processing factory effluent (MPE) in combination with additions of paunch to three arable sites and one pasture site on soil chemical and microbial properties were investigated in fields surrounding a beef meat processing factory. A pasture site that had only received MPE was also sampled along with adjoining arable and pasture control fields that had never received MPE or paunch. Additions of MPE/paunch caused increases in electrical conductivity, exchangeable Na and K, exchangeable sodium percentage (ESP), extractable P, organic C, total N, microbial biomass C, and metabolic quotient and decreases in exchangeable Ca and Mg, pH, and the proportion of organic C present as microbial biomass. The structure and diversity of bacterial and fungal communities was measured by polymerase chain reaction–denaturing gradient gel electrophoresis of 16S rDNA and internal transcribed spacer-RNA amplicons respectively and catabolic diversity by analysis of catabolic response profiles to 25 substrates. Principal component analysis of catabolic response profiles clearly separated control from MPE/paunch-treated sites, and this was associated with greater catabolic responses to the carboxylic acids α-ketoglutaric, α-ketobutyric, l-ascorbic, and citric acid in the control. At the arable sites, application of MPE and paunch caused increases in bacterial, fungal, and catabolic diversity. Canonical correspondence analysis of the relationship between catabolic, bacterial, and fungal fingerprints and soil properties indicated that the main soil variables separating MPE/paunch treatments from controls were the higher organic C, ESP, and extractable P and a lower pH, exchangeable Ca, and Mg. It was concluded that, although long-term MPE/paunch additions induce soil salinity, sodicity, and acidity, in general, they cause an increase in the size, activity, and structural and functional diversity of in the soil microbial community.

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References

  • Anderson, J. P. E. (1982). Soil Respiration. In A. L. Page (Ed.), Methods of soil analysis, part 2. Chemical and microbial properties (pp. 837–871). Madison, WI: American Society of Agronomy.

    Google Scholar 

  • Banks, C. J., & Wang, Z. (2006). Treatment of meat wastes. In L. K. Wang, Y. Hung, H. H. Lo, & C. Yapijakis (Eds.), Waste Treatment in the Food Processing Industry (pp. 67–100). Boca Raton, FL: Taylor & Francis.

    Google Scholar 

  • Beauregard, M. S., Hamel, C., Nayyar, A., & St-Arnaud, M. (2010). Long-term phosphorus fertilization impacts soil fungal and bacterial diversity but not AM fungal community in alfalfa. Microbial Ecology, 59, 379–389.

    Article  CAS  Google Scholar 

  • Bolan, N. S., & Hedley, M. (2003). Role of carbon, nitrogen and sulphur cycles in soil acidification. In Z. Rengel (Ed.), Handbook of soil acidity (pp. 29–56). New York: Marcel Dekker.

    Google Scholar 

  • Churchman, G. J., & Tate, K. R. (1986). Effect of slaughterhouse effluent and water irrigation upon aggregation in seasonally dry New Zealand soil under pasture. Australian Journal of Soil Research, 24, 505–516.

    Article  Google Scholar 

  • Colwell, J. D. (1963). The estimation of phosphorus fertilizer requirements of wheat in southern New South Wales by soil analysis. Australian Journal of Experimental Agriculture and Animal Husbandry, 3, 190–198.

    Article  CAS  Google Scholar 

  • Crecchio, C., Cuci, M., Mininni, R., Ricciuti, P., & Ruggiero, P. (2001). Short-term effects of municipal solid waste compost amendments on soil carbon and nitrogen content, some enzyme activities and genetic diversity. Biology and Fertility of Soils, 34, 311–318.

    Article  CAS  Google Scholar 

  • Degens, B. P., & Harris, J. A. (1997). Development of a physiological approach to measurement of metabolic diversity of soil microbial communities. Soil Biology and Biochemistry, 29, 1309–1320.

    Article  CAS  Google Scholar 

  • Degens, B. P., Schipper, L. A., Sparling, G. P., & Vojvodic-Vukovic, M. (2000). Decreases in organic C reserves in soils can reduce the catabolic diversity of soil microbial communities. Soil Biology and Biochemistry, 32, 189–196.

    Article  CAS  Google Scholar 

  • Degens, B. P., Schipper, L. A., Sparling, G. P., & Duncan, L. C. (2001). Is the microbial community in a soil with reduced catabolic diversity less resistant to stress or disturbance. Soil Biology and Biochemistry, 33, 1143–1153.

    Article  CAS  Google Scholar 

  • Fleming, R., & MacAlpine, M. (2004). Composting paunch manure with solid cattle manure. Report submitted to Blake Fisher Blaemer, Orangeville, Ontario. University of Guelph, Ridgetown, Ontario.

  • Giller, K. E., Beare, M. H., Lavelle, P., Izac, A.-M. N., & Swift, M. J. (1997). Agricultural intensification, soil biodiversity and agroecosystem function. Applied Soil Ecology, 6, 3–16.

    Article  Google Scholar 

  • Guo, L. B., & Sims, R. E. H. (2000). Effect of meat works effluent irrigation on soil, tree biomass production and nutrient uptake in Eucalyptus globulus seedlings in growth cabinets. Bioresource Technology, 72, 243–251.

    Google Scholar 

  • Haynes, R. J., & Beare, M. H. (1996). Aggregation and organic carbon storage in meso-thermal, humid soils. In M. R. Carter & B. A. Stewart (Eds.), Advances in Soil Science. Structure and Organic Matter Storage in Agricultural Soils (pp. 213–262). Boca Raton: CRC Lewis.

    Google Scholar 

  • Isbell, R. F. (2002). The Australian Soil Classification. Collingwood Victoria: CSIRO Publishing.

    Google Scholar 

  • Jarvis, N. J. (2007). A review of non-equilibrium water flow and solute transport in soil macropores: Principles, controlling factors and consequences for water quality. European Journal of Soil Science, 58, 523–546.

    Article  Google Scholar 

  • Liu, B., Gumpertz, M. L., Hu, S., & Ristaino, J. B. (2007). Long-term effects of organic and synthetic soil fertility amendments on soil microbial communities and the development of southern blight. Soil Biology and Biochemistry, 39, 2302–2316.

    Article  CAS  Google Scholar 

  • Liu, Y.-Y., & Haynes, R. J. (2011a). Origin, nature and treatment of effluents from dairy and meat processing factories and the effects of their irrigation on the quality of agricultural soils. Critical Reviews in Environmental Science and Technology, 41, 1531–1599.

    Article  CAS  Google Scholar 

  • Liu, Y.-Y., & Haynes, R. J. (2011b). Influence of land application of dairy factory effluent on soil nutrient status and the size, activity, composition and catabolic capability of the soil microbial community. Applied Soil Ecology, 48, 133–141.

    Article  Google Scholar 

  • Luo, J., Lindsey, S., & Xue, J. (2004). Irrigation of meat processing wastewater onto land. Agriculture, Ecosystems and Environment, 103, 123–148.

    Article  Google Scholar 

  • Marschner, P. (2007). Soil microbial community structure and function assessed by FAME, PLFA and DGGE. In A. Varma & D. G. Oelm (Eds.), Advanced Techniques in Soil Microbiology (Soil Biology) (pp. 181–200). Berlin: Springer-Verlag.

    Chapter  Google Scholar 

  • McBride, M. B. (1979). An interpretation of cation selectivity variations in M+-M+ exchange on clays. Clays and Clay Minererals, 27, 417–422.

    Article  CAS  Google Scholar 

  • Mittal, G. S. (2006). Treatment of wastewater from abattoirs before land application. Food Review International, 20, 229–256.

    Article  Google Scholar 

  • Muyzer, G., De Waal, E., & Uitterlinden, A. G. (1993). Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Applied and Environmental Microbiology, 59, 695–700.

    CAS  Google Scholar 

  • Nannipieri, P., Ascher, J., Ceccherini, M. T., Landi, L., Pietramellara, G., & Renella, G. (2003). Microbial diversity and soil function. European Journal of Soil Science, 54, 655–670.

    Article  Google Scholar 

  • Peet, R. K. (1974). The measurement of species diversity. Annual Review of Ecological Systems, 5, 285–308.

    Article  Google Scholar 

  • Rayment, G. E., & Higginson, F. R. (1992). Australian Laboratory Handbook of Soil and Water Chemical Methods. Melbourne: Inkata Press.

    Google Scholar 

  • Rietz, D. N., & Haynes, R. J. (2003). Effects of irrigation-induced salinity and sodicity on soil microbial activity. Soil Biology and Biochemistry, 35, 845–854.

    Article  CAS  Google Scholar 

  • Ross, D. J., Tate, K. R., Cairns, A., Meyrick, K. F., & Pansier, E. A. (1982). Effects of slaughterhouse effluent and water on biochemical properties of two seasonally dry soils under pasture. New Zealand Journal of Science, 25, 341–349.

    Google Scholar 

  • Rousk, J., Baath, E., Brookes, P. C., Lauber, C. L., Lozupone, C., Caporaso, J. G., Knight, R., & Fierer, N. (2010). Soil bacterial and fungal communities across a pH gradient in an arable soil. ISME Journal, 4, 1340–1351.

    Article  Google Scholar 

  • Russell, J. M., Cooper, R. N., & Lindsey, S. B. (1993). Soil denitrification rates at wastewater irrigation sites receiving primary-treated and anaerobically treated meat-processing effluent. Bioresource Technology, 43, 41–46.

    Google Scholar 

  • Sharma, S., Rangger, A., von Lutzow, M., & Insam, H. (1998). Functional diversity of soil microbial communities increases after maize litter amendment. European Journal of Soil Biology, 34, 53–60.

    Google Scholar 

  • Shaw, R. J. (1999). Soil salinity: Electrical conductivity and chloride. In K. I. Peverill, L. A. Sparrow, & D. J. Reuter (Eds.), Soil Analysis: An Interpretation Manual (pp. 129–145). Collingwood, Victoria: CSIRO Publishing.

    Google Scholar 

  • Stevenson, B. A., Sparling, G. P., Schipper, L. A., Degens, B. P., & Duncan, L. C. (2004). Pasture and forest soil microbial communities show distinct patterns in their catabolic respiration responses at a landscape scale. Soil Biology and Biochemistry, 36, 49–55.

    Article  CAS  Google Scholar 

  • Sumner, M. E. (1993). Sodic soils: New perspectives. Australian Journal of Soil Research, 31, 683–750.

    Article  Google Scholar 

  • van Oostrom, A. J. (2001). Waste management. In Y. H. Hui (Ed.), Meat Science and Application (pp. 635–669). Marcel Dekker: New York.

    Google Scholar 

  • Wakelin, S. A., Colloff, M. J., Harvey, P. R., Marschner, P., Gregg, A. L., & Rogers, S. L. (2007). The effects of stubble retention and nitrogen application on soil microbial community structure and functional gene abundance under irrigated maize. FEMS Microbial Ecology, 59, 661–670.

    Article  CAS  Google Scholar 

  • Wallis, P. D., Haynes, R. J., Hunter, C. H., & Morris, C. D. (2010). Effect of land use and management on soil bacterial biodiversity a measured by PCR-DGGE. Applied Soil Ecology, 46, 147–150.

    Article  Google Scholar 

  • Wardle, J. C., & Ghani, A. (1995). A critique of the microbial metabolic quotient (qCO2) as a bioindicator of disturbance and ecosystem development. Soil Biology and Biochemistry, 27, 1601–1610.

    Article  CAS  Google Scholar 

  • Wolters, V., & Joergensen, R. G. (1991). Microbial carbon turnover in beech forest soils at different stages of acidification. Soil Biology and Biochemistry, 23, 897–902.

    Article  Google Scholar 

  • Wu, J., Joergensen, R. G., Pommerening, B., Chaussod, R., & Brookes, P. C. (1990). Measurement of soil microbial biomass C by fumigation-extraction—An automated procedure. Soil Biology and Biochemistry, 22, 1167–1169.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We thank Dr Y-F Zhou for valuable assistance with PCR-DGGE analysis and catabolic diversity measurements, Del Greenway for statistical analysis and Paul Murray and Peter Harris for helping with choice of sites and sampling them.

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  1. School of Agriculture and Food Science/CRC CARE, The University of Queensland, St Lucia, Queensland, 4072, Australia

    Y.-Y. Liu & R. J. Haynes

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  1. Y.-Y. Liu
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  2. R. J. Haynes
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Correspondence to R. J. Haynes.

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Liu, YY., Haynes, R.J. Effect of Disposal of Effluent and Paunch from a Meat Processing Factory on Soil Chemical and Microbial Properties. Water Air Soil Pollut 224, 1655 (2013). https://doi.org/10.1007/s11270-013-1655-5

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