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Bioaccumulation, Elimination, and Toxic Effect of Cadmium on Structure of Gills and Hepatopancreas of Freshwater Prawn Macrobrachium sintangese (De Man, 1898)

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Abstract

The objectives of this study were to determine the acute toxicity of cadmium and to examine the bioaccumulation and elimination of cadmium in different tissues of the freshwater prawn Macrobrachium sintangese. It also evaluated the structural damage of gills and hepatopancreas of M. sintangese when administered to sublethal cadmium concentration and when exposed prawns were transferred to cadmium-free media. According to the mortality data, the 96 h LC50 value of Cd to M. sintangese was 86 μg/L. The highest cadmium accumulation was observed in gills, followed by the hepatopancreas, and the abdominal muscle. After being transferred to cadmium-free media, the highest cadmium elimination was observed in abdominal muscle, followed by the gills and hepatopancreas. The gills of prawns exposed to cadmium exhibited a severe hyperplasia, vacuolization, and multiple necroses which resulted to the swelling of lamellae. After transferring the cadmium-exposed prawns into the control media, the histopathological effects decreased. Severe alterations to the hepatopancreatic tissue were observed in prawns exposed to cadmium. The tubular epithelial cells were heavily vacuolated and even ruptured. The number of large vacuoles and R cells appeared in the tubular epithelial cells of the hepatopancreas. After transferring to the control media, the histological alterations of the hepatopancreas decreased. The tubular epithelial cells began to rearrange to the normal structure. The number of R cells and B cells were noted in the epithelial cells. The thickness of tubular epithelial cells was comparable to the controls. Due to the sensitivity of M. sintangese to cadmium, therefore this species potentially can be used as a test organism in toxicity assays.

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Acknowledgments

This research was partly supported by grants from the Ministry of Research and Technology of Indonesia. We are grateful to Miss A.N. Primarastri for histological work, Mrs. Setiyanto and Giman for the technical assistances during the experiments. We also wish to thank anonymous reviewers for valuable advice and constructive comments to improve the manuscript.

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Authors and Affiliations

  1. Department of Biology, Faculty of Sciences and Technology, Airlangga University, Kampus C, Jl. Mulyorejo, Surabaya, 60115, Indonesia

    Agoes Soegianto & Dwi Winarni

  2. Department of Chemistry, Faculty of Sciences and Technology, Airlangga University, Kampus C, Jl. Mulyorejo, Surabaya, 60115, Indonesia

    Usreg Sri Handayani &  Hartati

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  1. Agoes Soegianto
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Correspondence to Agoes Soegianto.

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Soegianto, A., Winarni, D., Handayani, U.S. et al. Bioaccumulation, Elimination, and Toxic Effect of Cadmium on Structure of Gills and Hepatopancreas of Freshwater Prawn Macrobrachium sintangese (De Man, 1898). Water Air Soil Pollut 224, 1575 (2013). https://doi.org/10.1007/s11270-013-1575-4

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