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Assessing the Oxidative Stress Induced by Paracetamol Spiked in Artificial Sediment on Hyalella azteca

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Abstract

Paracetamol is an antipyretic analgesic widely used globally. It has been recurrently found in water bodies and is known to elicit toxic effects in aquatic species; however, its potential ability to induce oxidative stress in sentinel species remains unknown The objective was to establish a methodology to evaluate the toxicity elicited on the sentinel species Hyalella azteca by paracetamol-enriched sediment using oxidative stress tests. Concentrations used in assays were determined using the previously obtained median lethal concentration (72 h LC50). The following oxidative stress biomarkers were evaluated: lipid peroxidation (LPO), protein carbonyl content (PCC) in order to determine oxidized protein content, and the activity of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX). LPO and PCC increased significantly while SOD, CAT, and GPX decreased significantly (p < 0.05) with respect to controls. Paracetamol induces oxidative stress on H. azteca, and the set of tests employed is helpful in evaluating the toxicity of this group of pharmaceuticals on aquatic species.

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Acknowledgments

This study was made possible through support from the Secretary of Research and Advanced Studies of the Autonomous University of Mexico State by means of Project 2971/2010SF.

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Authors and Affiliations

  1. Laboratorio de Toxicología Ambiental, Departamento de Farmacia, Facultad de Química, Universidad Autónoma del Estado de México, Toluca, Mexico

    Leobardo Manuel Gómez-Oliván, Nadia Neri-Cruz & Octavio Dublán-García

  2. Laboratorio de Toxicología Acuática, Departamento de Farmacia, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico

    Marcela Galar-Martínez, Sandra García-Medina & Celene Razo-Estrada

  3. Laboratorio de Neurofisiología de la Conducta, Facultad de Medicina, Universidad Autónoma del Estado de México, Toluca, Mexico

    Patricia Vieyra-Reyes

  4. Laboratorio de Ciencias Ambientales, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Ciudad Juárez, Mexico

    Alba Yadira Corral-Avitia

  5. Laboratorio de Toxicología, Departamento de Farmacia, Universidad Autónoma del Estado de México, Paseo Colón intersección Paseo Tollocan, s/n. Col. Residencial Colón, Toluca, Estado de México, C.P. 50120, Mexico

    Leobardo Manuel Gómez-Oliván

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  1. Leobardo Manuel Gómez-Oliván
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  2. Nadia Neri-Cruz
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Correspondence to Leobardo Manuel Gómez-Oliván.

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Gómez-Oliván, L.M., Neri-Cruz, N., Galar-Martínez, M. et al. Assessing the Oxidative Stress Induced by Paracetamol Spiked in Artificial Sediment on Hyalella azteca . Water Air Soil Pollut 223, 5097–5104 (2012). https://doi.org/10.1007/s11270-012-1261-y

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  • DOI: https://doi.org/10.1007/s11270-012-1261-y

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