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Production of Bioflocculant by Staphylococcus cohnii ssp. from Palm Oil Mill Effluent (POME)

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Staphylococcus cohnii ssp. was found to produce a bioflocculant with high flocculating activity for kaolin suspension. Using optimized culture conditions, a flocculating activity of 70.3 % at a bioflocculation concentration of 0.3 mg/L at pH 7 with Ca2+ as the cation and 88.9 % in 1.2 mg/L of bioflocculant concentration for Al3+ as the cation was obtained for bacteria that were incubated at temperature of 40 °C for 2-day incubation period. It was found to be effective for flocculation of a kaolin suspension over weakly acidic pH (6–8); divalent and trivalent (Ca2+ and Al3+) enhanced the flocculating activity. This bioflocculant possesses a good flocculating activity, which can be promoted by the addition of trivalent and bivalent cations in kaolin suspension.

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AlCl3 :

Aluminum chloride


Aluminum sulphate


Bioflocculant-producing bacteria strain type 3


Degree celsius

CaCl2 :

Calcium chloride




Potassium chloride






Magnesium sulphate heptahydrate




Palm oil mill effluent


Scanning electron microscope




Single species

w/v :

Weight over volume


  • Deng, S., Yu, G., & Ting, Y. P. (2005). Production of a bioflocculant by Aspergillus parasiticus and its application in dye removal. Colloids Surface Journal, 44, 179–186.

    Article  CAS  Google Scholar 

  • Fujita, M., Ike, M., Jang, J. H., Kim, S. M., & Hirao, T. (2001). Bioflocculant production from lower-molecular fatty acids as a novel strategy for utilization of sludge digestion liquor. Water Science Technology Journal, 44, 237–243.

    CAS  Google Scholar 

  • Fujita, M., Ike, M., Tachibana, S., Kitada, G., Kim, S. M., & Inoue, Z. (2000). Characterization of a bioflocculant produced by Citrobacter sp. TKF04 from acetic and propionic acids. Journal of Bioscience Bioengineering, 89(1), 40–46.

    Article  CAS  Google Scholar 

  • Gong, W. X., Wang, S. G., Sun, X. F., Liu, X. W., Yue, Q. Y., & Gao, B. Y. (2008). Bioflocculant production by culture of Serratia ficaria and its application in wastewater treatment. Bioresource Technology Journal, 99(11), 4668–4674.

    Article  CAS  Google Scholar 

  • He, N., Li, Y., & Chen, J. (2004). Production of a novel polygalacturonic acid bioflocculant REA-11 by Corynebacterium lutamicum. Bioresource Technology Journal, 94, 99–105.

    Article  CAS  Google Scholar 

  • Lawson, C.J. and Suntherland, I.W., (1978) Polysaccharide In. A H. Rose (Ed.), Economic microbiology, primary products of metabolism, Vol. 2, Academic, London, p 328-389.

  • Levy, N., Magdasi, S., & Bar-Or, Y. (1992). Physico-chemical aspects in flocculation of bentonite suspensions by a cyanobacterial bioflocculant. Water Resource Journal, 26, 249–254.

    CAS  Google Scholar 

  • Low, L. W., Teng, T. T., Anees, A., Morad, N., & Wong, Y. S. (2011). A novel pretreatment method of lignocellulosic material as adsorbent and kinetic study of dye waste adsorption, Journal of Water. Air and Soil Pollution, 218(1–4), 293–306.

    Article  CAS  Google Scholar 

  • Lu, W. Y., Zhang, T., Zhang, D. Y., Li, C. H., Wen, J. P., & Du, L. X. (2005). A novel bioflocculant produced by Enterobacter aerogenes and its use in defecating the trona suspension. Biochemical Engineering Journal, 27, 1–7.

    Article  Google Scholar 

  • Nohata, Y., & Kurane, R. (1991). Microbial flocculation of waste liquids and oil emulsion by a bioflocculant from Alcaligenes latus. Agriculture Biological Chemical Journal, 55, 1127–9.

    Article  Google Scholar 

  • Ong, S.-A., Ho, L.-N., Wong, Y.-S., Danny, L. G., & Hafizah, S. (2011). Semi-batch operated constructed wetlands planted with Phragmites australis for treatment of dyeing wastewater. Journal of Engineering Science and Technology, 6(5), 623–631.

    Google Scholar 

  • Ong, S.-A., Ho, L.-N., Wong, Y.-S., & Raman, K. (2012). Performance and kinetic study on bioremediation of diazo dye (Reactive Black 5) in wastewater using spent GAC–biofilm sequencing batch reactor. Journal of Water, Air and Soil Pollution.. doi:10.1007/s11270-011-0969-4.

  • Pang, M. P., Kumar, P., Teng, T. T., Mohd Omar, A. K., & Kailas, L. W. (2011). Removal of lead, zinc and iron by coagulation–flocculation. Journal of the Taiwan Institute of Chemical Engineers, 42, 809–815.

    Article  CAS  Google Scholar 

  • Salehizadeh, H., & Shojaosadati, S. A. (2001). Extracellular biopolymeric flocculants: recent trends and biotechnological importance. Biotechnology Advance Journal, 19, 371–385.

    Article  CAS  Google Scholar 

  • Sheng, Y. L., Zhang, Q. A., Sheng, Y. R., Li, C. B., & Wang, H. J. (2006). Screening and flocculating properties of bioflocculant-producing microorganisms. Journal of University Science Technology Beijing, 13(4), 289–292.

    CAS  Google Scholar 

  • Shih, I. L., Van, Y. T., Yeh, L. C., Lin, H. G., & Chang, Y. N. (2001). Production of a biopolymer flocculant from Bacillus licheniformis and its flocculation properties. Bioresource Technology Journal, 78, 267–272.

    Article  CAS  Google Scholar 

  • Suh, H. H., Kwon, G. S., Lee, C. H., Kim, H. S., Oh, H. M., & Yoon, B. D. (1997). Characterization of bioflocculant produced by Bacillus sp. DP-152. Journal of Fermentation Bioengineering, 84, 108–12.

    Article  CAS  Google Scholar 

  • Wong, Y. S., Kadir, Mohd Omar Ab, & Teng, T. T. (2009). Biological kinetics evaluation of anaerobic stabilization pond treatment of palm oil mill effluent. Journal of Bioresource Technology, 100, 4969–4975.

    Article  CAS  Google Scholar 

  • Wu, J. Y., & Ye, H. F. (2007). Characterization and flocculating properties of an extracellular biopolymer produced from a Bacillus subtilis DYU 1 isolate. Process Biochemistry Journal, 42, 1114–1123.

    Article  CAS  Google Scholar 

  • Yim, J. H., Kim, S. J., Ahn, S. H., & Lee, H. K. (2007). Characterization of a novel bioflocculant, p-KG03, from a marine dinoflagellate, Gyrodinium impudicum KG03. Bioresource Technology Journal, 98, 361–367.

    Article  CAS  Google Scholar 

  • Yokoi, H., Yoshida, T., Mori, S., Hirose, J., Hayashi, S., & Takasaki, Y. (1997). Biopolymer flocculant produced by Enterobacter sp. Biotechnology Letter, 19, 569–73.

    Article  CAS  Google Scholar 

  • Zhang, Z. Q., Lin, B., Xia, S. Q., Wang, X. J., & Yang, A. M. (2007). Production and application of a bioflocculant by multiple-microorganism consortia using brewery wastewater as carbon source. Journal of Environmental Science, 19, 660–666.

    Google Scholar 

  • Zhang, J., Wang, R., Jiang, P., & Liu, Z. (2002). Production of an exopolysaccharide bioflocculant by Sorangium cellulosum. Letter Applied Microbiology Journal, 34, 178–181.

    Article  Google Scholar 

  • Zouboulis, A. I., Chai, X. L., & Katsoyiannis, I. A. (2004). The application of bioflocculant for the removal of humic acids from stabilized landfill leachates. Journal of Environmental Management, 70, 35–41.

    Article  Google Scholar 

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The authors would like to express their thanks for the support of the Project for School of Environmental Engineering of the University Malaysia Perlis (UniMAP), MALPOM Industries Sdn Bhd and financial support from UniMAP short-term grant and Malaysian Government Institution of Higher Education grant.

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Correspondence to Yee-Shian Wong.

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Wong, YS., Ong, SA., Teng, TT. et al. Production of Bioflocculant by Staphylococcus cohnii ssp. from Palm Oil Mill Effluent (POME). Water Air Soil Pollut 223, 3775–3781 (2012).

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