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Wastewater Management from Slaughterhouse—A New Approach to Control Mosquitoes

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Abstract

Slaughterhouse waste water (SHW) is discarded as unused disposals into the environment every day. The objective of the present study is to explore the possibility on the utilization of SHW for the preparation of culture media to produce mosquitocidal bacterium (Bacillus thuringiensis subsp. israelensis). This would help to overcome the problem of dumping SHW in to environment. A judicial combination of SHW with a mineral salt (MnCl2) was made to produce an enhanced level of bacterial production when compared with other culture media including conventional medium (Luria Bertani, LB). A complete degradation of SHW by the bacteria was observed. The biomass yield, bacterial growth, toxin production, and larvicidal activity against mosquito vectors were satisfactory. Cell mass yield of 4.55 gm l−1 (dry wt) and larvicidal activity of 0.006 mg ml−1 and 0.026 mg ml−1 at LC50 and LC90 levels were observed, respectively, against the filarial vector of Culex quinquefasciatus with bacteria grown in SHW + MnCl2. The B. thuringiensis subsp. israelensis also controlled the larvae in the field significantly for three weeks (>90% mortality) and the effect was comparable with LB. Cost-analysis for production of B. thuringiensis subsp. israelensis showed that it is more economical. Thus, this study suggested the dual benefit of efficient production of mosquitocidal toxin and management of slaughterhouse wastewater.

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Acknowledgments

This study was supported by a research grant from the Department of Science and Technology (DST), Govt of India (SR/FTP/LS-A-86/2001). We thank, the director of VCRC, Pondicherry for permission, Dr. J-F Charles, Bacteries Entomopathogenes, Institute Pasteur, Paris, France for supply of bacterial strain, and Smt. R. Sundarammal, Senior Library Information Officer, VCRC, Pondicherry for literatures pertinent to the study.

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  1. Unit of Microbiology and Immunology, Vector Control Research Centre (Indian Council of Medical Research), Medical complex, Indira Nagar, Pondicherry, 60 5006, India

    Subbiah Poopathi & Balaraman Archana

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  1. Subbiah Poopathi
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  2. Balaraman Archana
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Correspondence to Subbiah Poopathi.

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Poopathi, S., Archana, B. Wastewater Management from Slaughterhouse—A New Approach to Control Mosquitoes. Water Air Soil Pollut 223, 3263–3270 (2012). https://doi.org/10.1007/s11270-012-1107-7

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