Following the Deepwater Horizon explosion and crude oil contamination of a marsh ecosystem in AL in June 2010, hydrocarbon-degrader microbial abundances of aerobic alkane, total hydrocarbon, and polycyclic aromatic hydrocarbon (PAH) degraders were enumerated seasonally. Surface sediment samples were collected in October and December of 2010 and in April and July of 2011 along 40–70-m transects from the high tide to the intertidal zone including Spartina alterniflora-vegetated marsh, seagrass (Ruppia maritima)-dominated sediments, and nonvegetated sediments. Alkane and total hydrocarbon degraders in the sediment were detected, while PAH degraders were below detection limit at all locations examined during the sampling periods. The highest counts for microbial alkane degraders were observed at the high tide line in April and averaged to 8.65 × 105 of cells/g dry weight (dw) sediment. The abundance of alkane degraders during other months ranged from 9.49 × 103 to 3.87 × 104, while for total hydrocarbon degraders, it ranged between 5.62 × 103 and 1.14 × 105 of cells/g dw sediment. Pore water nutrient concentrations (NH +4 , NO −3 , NO −2 , and PO 3−4 ) showed seasonal changes with minimum values observed in December and April and maximum values in October and July. Concentrations of total petroleum hydrocarbons in sediments averaged 100.4 ± 52.4 and 141.9 ± 57.5 mg/kg in January and July, 2011, respectively. The presence of aerobic microbial communities during all seasons in these nearshore ecosystems suggests that an active and resident microbial community is capable of mineralizing a fraction of petroleum hydrocarbons.
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Alexander, M. (1999). Biodegradation and bioremediation (2nd ed.). San Diego: Academic.
Atlas, R. M. (1995a). Bioremediation of petroleum pollutants. International Biodeterioration & Biodegradation, 35(1–3), 317–327.
Atlas, R. M. (1995b). Petroleum biodegradation and oil spill bioremediation. Marine Pollution Bulletin, 31(4–12), 178–182.
Atlas, R. M., & Bartha, R. (1998). Microbial interactions with xenobiotic and inorganic pollutants. Microbial Ecology Fundamentals and Applications (4th ed.). Menlo Park: Benjamin/Cummings Science Publishing.
Azwell, T., Blum, M. J., Hare, A., Joye, S., Kubendran, S., Laleian, A., et al. (2011). The Macondo blowout environmental report. Deepwater Horizon Study Group (pp. 1-9). Berkley, CA.
Boufadel, M. C., Reeser, P., Suidan, M. T., Wrenn, B. A., Cheng, J., Du, X., et al. (1999). Optimal nitrate concentration for the biodegradation of n-heptadecane in a variably-saturated sand column. Environmental Technology, 20, 191–199.
Braddock, J. F., Ruth, M. L., Catterall, P. H., Walworth, J. L., & McCarthy, K. A. (1997). Enhancement and inhibition of microbial activity in hydrocarbon-contaminated Arctic soils: implications for nutrient-amended bioremediation. Environmental Science & Technology, 31(7), 2078–2084.
Brown, E. J., & Braddock, J. F. (1990). Sheen screen, a miniaturized most-probable-number method for enumeration of oil-degrading microorganisms. Applied and Environmental Microbiology, 56(12), 3895–3896.
Burke, D. J., Hamerlynck, E. P., & Hahn, D. (2003). Interactions between the salt marsh grass Spartina patens, arbuscular mycorrhizal fungi and sediment bacteria during the growing season. Soil Biology & Biochemistry, 35, 501–511.
Crone, T. J., & Tolstoy, M. (2010). Magnitude of the 2010 Gulf of Mexico oil leak. Science, 330(6004), 634–634.
Daane, L. L., Harjono, I., Zylstra, G. J., & Haggblom, M. M. (2001). Isolation and characterization of polycyclic aromatic hydrocarbon-degrading bacteria associated with the rhizosphere of salt marsh plants. Applied and Environmental Microbiology, 67(6), 2683–2691.
de Man, J. C. (1983). MPN tables, corrected. European Journal of Applied Microbiology and Biotechnology, 17, 301–305.
DeFlaun, M., & Mayer, L. (1983). Relationships between bacteria and grain surfaces in intertidal sediments. American Society of Limnology and Oceanography, 28(5), 873–881.
Elmendorf, D. L., Haith, C. E., Douglas, G. S., & Prince, R. C. (1994). Relative rates of biodegradation of substituted polycyclic aromatic hydrocarbons. In R. E. Hinchee, A. Leeson, L. Semprini, & S. K. Ong (Eds.), Bioremediation of chlorinated and polycyclic aromatic hydrocarbon compounds. Boca Raton: Lewis Publishers.
Enock, J. (2002). Intrinsic biodegradation potential of crude oil in salt marshes. Louisiana State University and Agricultural and Mechanical College
Haines, J. R., Wrenn, B. A., Holder, E. L., Strohmeier, K. L., Herrington, R. T., & Venosa, A. D. (1996). Measurement of hydrocarbon-degrading microbial populations by a 96-well plate most-probable-number procedure. Journal of Industrial Microbiology and Biotechnology, 16(1), 36–41.
Hazen, T. C., Dubinsky, E. A., DeSantis, T. Z., Andersen, G. L., Piceno, Y. M., Singh, N., et al. (2010). Deep-see oil plume enriches indigenous oil-degrading bacteria. Science, 330(6001), 204–208.
Hood, M. A., Bishop, W. S., Meyers, S. P., & Whelan, T., III. (1975). Microbial indicators of oil-rich salt marsh sediments. Applied Microbiology, 30(6), 982–987.
Horel, A., & Schiewer, S. (2009). Investigation of the physical and chemical parameters affecting biodegradation of diesel and synthetic diesel fuel contaminating Alaskan soils. Cold Regions Science and Technology, 58(3), 113–119.
Horel, A., & Schiewer, S. (2011). Influence of constant and fluctuating temperature on biodegradation rates of fish biodiesel blends contaminating Alaskan sand. Chemosphere, 83(5), 652–660.
Jackson, A. W., & Pardue, J. H. (1998). Potential for enhancement of biodegradation of crude oil in Louisiana salt marshes using nutrient amendments. Water, Air, and Soil Pollution, 104(1–4), 343–355.
Kostka, J. E., Prakash, O., Overholt, W. A., Green, J. S., Freyer, G., Canion, A., et al. (2011). Hydrocarbon-degrading bacteria and the bacterial community response in Gulf of Mexico beach sands impacted by the Deepwater Horizon oil spill. Applied and Environmental Microbiology, 77(22), 7962–7974.
LaRiviere, D. J., Autenrieth, R. L., & Bonner, J. S. (2003). Redox dynamics during recovery of an oil-impacted estuarine wetland. Water Research, 37, 3307–3318.
Launen, L. A., Dutta, J., Turpeinen, R., Eastep, M. E., Dorn, R., Buggs, V. H., et al. (2008). Characterization of the indigenous PAH-degrading bacteria of Spartina dominated salt marshes in the New York/New Jersey Harbor. Biodegradation, 19, 347–363.
Leahy, J. G., & Colwell, R. R. (1990). Microbial-degradation of hydrocarbons in the environment. Microbiological Reviews, 54(3), 305–315.
Lin, Q., & Mendelssohn, I. A. (1998). The combined effects of phytoremediation and biostimulation in enhancing habitat restoration and oil degradation of petroleum contaminated wetlands. Ecological Engineering, 10, 263–274.
MacDonald, I. R., Leifer, I., Sassen, R., Stine, P., Mitchell, R., & Guinasso, N., Jr. (2002). Transfer of hydrocarbons from natural seeps to the water column and atmosphere. Geofluids, 2, 95–107.
Maier, R. M., & Pepper, I. L. (2009). Earth environments. In R. M. Maier, I. L. Pepper, & C. P. Gerba (Eds.), Environmental microbiology (2nd ed., pp. 57–82). Burlington: Elsevier.
Mearns, A. J., Venosa, A. D., Lee, K., & Salazar, M. (1997). Field-testing bioremediation treating agents: lessons from an experimental shoreline oil spill. Paper presented at the International Oil Spill Conference, Fort Lauderdale, Florida.
Mills, M. A., Bonner, J. S., McDonald, T. J., Page, C. A., & Autenrieth, R. L. (2003). Intrinsic bioremediation of petroleum-impacted wetland. Marine Pollution Bulletin, 46, 887–899.
Morris, J. T. (2006). Competition among marsh macrophytes by means of geomorphological displacement in the intertidal zone. Estuarine and Coastal Shelf Science, 69, 395–402.
Phillips, L. A. (2008). The relationship between plants and their root-associated microbial communities in hydrocarbon phytoremediation. Dissertation, University of Saskatchewan, Saskatoon.
Porter, K. G., & Feig, Y. S. (1980). The use of DAPI for identifying and counting aquatic microflora. Limnology and Oceanography, 25(5), 943–948.
Saadoun, I. M. K., & Al-Ghzawi, Z. D. (2005). Bioremediation of petroleum contamination (Bioremediation of Aquatic and Terrestial Ecosystems): Science Publishers Inc.
Stephens, D. B. (2000). Vadoze zone hydrology. Boca Raton: CRC Press, Inc.
Stout, J. (1984). The ecology of irregularly flooded salt marshes of the northeastern Gulf of Mexico: a community profile (U. S. D. o. t. Interior, Trans.). Biological Report (Vol. 85, pp. 1-115): Fish and Wildife Service
Walworth, J., & Ferguson, S. (2008). Nutrient requirements for bioremediation. In D. Filler, I. Snape, & D. L. Barnes (Eds.), Bioremediation of petroleum hydrocarbons in cold regions: Cambridge University Press.
Wrenn, B. A., & Venosa, A. D. (1996). Selective enumeration of aromatic and aliphatic hydrocarbon degrading bacteria by a most-probable-number procedure. Canadian Journal of Microbiology, 42(3), 252–258.
Wright, A. L., Weaver, R. W., & Webb, J. W. (1996). Oil bioremediation in salt marsh mesocosms as infuenced by N and P fertilization, flooding, and season. Water, Air, and Soil Pollution, 95(1–4), 179–191.
Zhu, X., Venosa, A. D., Suidan, M. T., & Lee, K. (2004). Guidelines for the bioremediation of oil-contaminated salt marshes (pp. 1–66). Cincinnati: Environmental Protection Agency.
This material is based upon the work supported by the National Science Foundation-CBET under grant no. RAPID 1042743, Mississippi State University/Northern Gulf Institute 191001-306911_01/TO 091, and Dauphin Island Sea Lab/Marine Environmental Science Consortium 2423 Jv, T4-005UA. The authors thank A. Ortmann and B. Christiaen for instrument use and assistance.
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Horel, A., Mortazavi, B. & Sobecky, P.A. Seasonal Monitoring of Hydrocarbon Degraders in Alabama Marine Ecosystems Following the Deepwater Horizon Oil Spill. Water Air Soil Pollut 223, 3145–3154 (2012). https://doi.org/10.1007/s11270-012-1097-5
- Deepwater Horizon
- Crude oil
- Hydrocarbon degraders
- Macondo well
- Salt marsh