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Effects of Endo- and Ectomycorrhizal Fungi on Physiological Parameters and Heavy Metals Accumulation of Two Species from the Family Salicaceae

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Abstract

There is increasing interest in poplars and willows due to their biomass production and phytoremediation potential. They host two major types of mycorrhizal fungi that can substantially modulate the physiology of their hosts. In this study, the effects of endo- and ectomycorrhizal fungi on growth, physiological parameters, and heavy metals accumulation were studied in a pot experiment using Salix alba L. and Populus nigra L. The mycorrhizal fungi were inoculated separately and in combination to a soil substrate polluted by a mixture of heavy metals (mainly Cd, Pb, and Zn). Tree species differed in their mycorrhizal affinities, with poplar being colonized predominantly by Glomus intraradices and willow by Hebeloma mesophaeum. H. mesophaeum increased willow height and biomass, while G. intraradices decreased poplar height. The photosynthetic rate remained unchanged, and only minor changes were observed in the relative composition of photosynthetic pigments. Poplar photosynthetic rates and levels of photosynthetic pigments declined, while the epicuticular waxes in leaves increased toward the end of the experiment, irrespective of the inoculation. H. mesophaeum strongly reduced the accumulation of Cd and Fe in willow and poplar shoots, respectively. Our results support the use of selected mycorrhizal strains to tune phytoremediation outcomes in their plant hosts.

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Acknowledgments

We greatly acknowledge the support of the research by a grant from Norway through the Norwegian Financial Mechanism (project no. CZ0092), by the Ministry of Education, Youth and Sports of the Czech Republic (MSM 6046137307), and by the Academy of Sciences of the Czech Republic (grant AV0Z60050516). We also thank Dr. Radka Sudová for the selection of the AM strains, Mgr. Pavla Doubková for the preparation of the AM inocula, and Dr. Andrea Polle for kindly providing us the isolate P. involutus Maj.

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Authors and Affiliations

  1. Department of Mycorrhizal Symbioses, Institute of Botany, Academy of Sciences of the Czech Republic, Zámek 1, 252 43, Průhonice, Czech Republic

    Libor Mrnka, Michal Kuchár & Miroslav Vosátka

  2. Department of Analytical Chemistry, Institute of Chemical Technology, Prague, Technická 5, 166 28, Prague 6-Dejvice, Czech Republic

    Zuzana Cieslarová & Pavel Matějka

  3. Department of Agroenvironmental Chemistry and Plant Nutrition, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 21, Prague 6-Suchdol, Czech Republic

    Jiřina Száková & Pavel Tlustoš

  4. Department of Mycorrhizal Symbioses, Institute of Botany, Academy of Sciences of the Czech Republic, Lesní 322, 252 43, Průhonice, Czech Republic

    Libor Mrnka

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  1. Libor Mrnka
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  2. Michal Kuchár
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  3. Zuzana Cieslarová
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Correspondence to Libor Mrnka.

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Mrnka, L., Kuchár, M., Cieslarová, Z. et al. Effects of Endo- and Ectomycorrhizal Fungi on Physiological Parameters and Heavy Metals Accumulation of Two Species from the Family Salicaceae. Water Air Soil Pollut 223, 399–410 (2012). https://doi.org/10.1007/s11270-011-0868-8

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