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Manure as a Source of Antibiotic-Resistant Escherichia coli and Enterococci: a Case Study of a Wisconsin, USA Family Dairy Farm

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Abstract

The spread of antibiotic-resistant bacteria in the environment is raising serious public health concerns, and manure is being increasingly recognized as a major source of antibiotic-resistant bacteria. In this research, we isolated Escherichia coli and enterococci from manure produced in a Wisconsin, USA family dairy farm to determine their resistance to six representative antibiotics. The average densities for E. coli and enterococci were 6.37(±4.38) × 107 colony formation units (CFU) g−1 and 1.60(±1.57) × 104 CFU g−1, respectively. The E. coli isolates were found to be resistant to cephalothin, ampicillin, tetracycline, and erythromycin. In addition to these four antibiotics, the Enterococcus isolates were also resistant to gentamicin and ciprofloxacin. Additionally, we examined the survival and growth of E. coli and enterococci in dairy manure over a period of ~3 days. While the densities of enterococci remained stable over the study period, the concentrations of E. coli on average increased by 1.5 log10 units. Further tests of the bacterial antibiotic resistance over time showed no significant changes in the prevalence of antibiotic resistance. This result indicated that slightly aged manure could represent a larger source of antibiotic-resistant E. coli than fresh manure and the accumulation of antibiotic-resistant E. coli and enterococci in the agricultural fields must be accounted for in the modeling of the spread of antibiotic-resistant bacteria in the environment.

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Acknowledgment

We are grateful to two anonymous reviewers whose comments and suggestions improved our manuscript.

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  1. Department of Geosciences, University of Wisconsin—Milwaukee, Milwaukee, WI, 53211, USA

    Jacob J. Walczak & Shangping Xu

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  1. Jacob J. Walczak
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Correspondence to Shangping Xu.

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Walczak, J.J., Xu, S. Manure as a Source of Antibiotic-Resistant Escherichia coli and Enterococci: a Case Study of a Wisconsin, USA Family Dairy Farm. Water Air Soil Pollut 219, 579–589 (2011). https://doi.org/10.1007/s11270-010-0729-x

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