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Cinnamaldehyde Induces PCD-Like Death of Microcystis aeruginosa via Reactive Oxygen Species

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Abstract

In recent years, Microcystis bloom occurs frequently and causes a wide range of social, environmental, and economic problems. In this study, dose-dependent inhibitory effect of cinnamaldehyde on the growth of Microcystis aeruginosa was investigated. It was found that cinnamaldehyde with the concentration more than 0.6 mM showed algicide activity against M. aeruginosa. When M. aeruginosa was exposed to 0.6 mM cinnamaldehyde, considerable reactive oxygen species (ROS) were generated followed by lipid peroxidation and decrease in the content of both chlorophyll a and soluble protein. Although superoxide dismutase had made response to the stress caused by cinnamaldehyde, activity increasing after a time of lag could not prevent the lysis of M. aeruginosa cells. Interestingly, the addition of antioxidants glutathione and l-ascorbic acid (Vc) could prevent the lysis of M. aeruginosa cells. All the results suggested that cinnamaldehyde induced the death of M. aeruginosa cells via inducing ROS burst. Further understanding of the mechanism of cinnamaldehyde-induced M. aeruginosa cell death would contribute to the control of cyanobacteria pollution.

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Acknowledgement

This work was supported by the Special Project of National Grand Fundamental Research Pre-973 Program of China under grant no. 2008CB117001.

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Authors and Affiliations

  1. School of Food Science, Henan Institute of Science and Technology, Xinxiang, 453003, China

    Liang Bin Hu

  2. Institute of food safety, Jiangsu Academy of Agricultural Sciences, No.50 Zhongling Street, Outside the Zhongshan Men, Nanjing, 210014, China

    Liang Bin Hu, Wei Zhou, Jing Dong Yang, Jian Chen, Yu Fen Yin & Zhi Qi Shi

  3. Key Laboratory of Food Safety Monitoring and Management Ministry of Agriculture, People’s Republic of China, Nanjing, 210014

    Liang Bin Hu, Wei Zhou, Jing Dong Yang & Zhi Qi Shi

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  1. Liang Bin Hu
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  2. Wei Zhou
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  3. Jing Dong Yang
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  4. Jian Chen
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  5. Yu Fen Yin
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  6. Zhi Qi Shi
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Correspondence to Zhi Qi Shi.

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Liang Bin Hu and Wei Zhou contributed equally to this work.

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Hu, L.B., Zhou, W., Yang, J.D. et al. Cinnamaldehyde Induces PCD-Like Death of Microcystis aeruginosa via Reactive Oxygen Species. Water Air Soil Pollut 217, 105–113 (2011). https://doi.org/10.1007/s11270-010-0571-1

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  • DOI: https://doi.org/10.1007/s11270-010-0571-1

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