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Water, Air, & Soil Pollution

, Volume 216, Issue 1–4, pp 39–49 | Cite as

Responses of Biochemical Markers in the Fish Prochilodus lineatus Exposed to a Commercial Formulation of Endosulfan

  • Carla Bacchetta
  • Jimena Cazenave
  • María Julieta ParmaEmail author
Article

Abstract

Among the most extensively used compounds for the pest control in Argentinean crops is the organochlorine endosulfan. The sublethal effects of the commercial endosulfan formulation on hematology and lipid peroxidation (LPO) of the neotropical fish Prochilodus lineatus were investigated. Firstly, we calculated acute toxicity (LC50) in order to define sublethal concentrations (0, 1.2, and 2.4 μg L−1). Hematological and oxidative stress responses were assessed at 24, 48, and 96 h. Endosulfan exposure significantly diminished the hemoglobin concentration, mean cell hemoglobin, and total plasma protein and increased white blood cells count and plasma glucose after 96 h. Exposed fish showed an alteration of the differential leukocytes count, evidenced by more thrombocytes and monocytes and less lymphocytes and neutrophils. Endosulfan increased LPO levels in intestine, liver, and brain in both sublethal concentrations. The present results suggest that endosulfan produces biochemical and physiological alterations, including immunological disorders, and it is a good inductor of oxidative stress in P. lineatus.

Keywords

Fish Biomarkers Hematology Oxidative stress Pesticide 

Notes

Acknowledgments

This work was supported by grants from Agencia Nacional de Promoción Científica y Técnica (PICT-1764), Consejo de Investigaciones Científicas y Técnicas (PIP-11420090100178), and Universidad Nacional del Litoral (CAI+D). We thank Wiener Lab for providing some kits for analysis. Authors wish to thank to Regner, Lordi, and Creus for their collaboration during field work.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Carla Bacchetta
    • 1
  • Jimena Cazenave
    • 1
    • 2
  • María Julieta Parma
    • 1
    • 2
    Email author
  1. 1.Laboratorio de IctiologíaInstituto Nacional de Limnología (INALI-CONICET-UNL)Santa FeArgentina
  2. 2.Departamento de Ciencias Naturales, Facultad de Humanidades y CienciasUniversidad Nacional del Litoral (FHUC-UNL)Santa FeArgentina

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