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Differences in Ozone Sensitivity at Different NPK Levels of Three Tropical Varieties of Mustard (Brassica campestris L.): Photosynthetic Pigments, Metabolites, and Antioxidants

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Abstract

The effects of ambient O3 at two different levels of nitrogen, phosphorus, and potassium, (recommended and 1.5 times the recommended NPK) on three tropical varieties of mustard (Brassica campestris L. var. Kranti, Aashirwad and Vardan) were explored to unravel the mechanism of protection under higher NPK level at a rural experimental site using open top chambers. Ambient O3 concentrations ranged from 27.7 to 59.04 ppb. Lipid peroxidation, antioxidative enzymes, and metabolites were higher, whereas photosynthetic pigments and protein were lower in all the varieties of mustard grown in non-filtered chambers than in filtered chambers. The magnitude of response varied with varieties, NPK levels and ages. Vardan showed a maximum stimulation in the antioxidative defense system, thus efficient scavenging of ROS produced by O3 and consequently conferred greater tolerance in terms of least reductions in pigments and protein as compared to Kranti and Aashirwad. The antioxidant defense system was not stimulated in response to 1.5 times the recommended NPK, but higher levels of pigments and protein were maintained compared to the recommended NPK under ambient O3 levels.

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Abbreviations

OTCs:

Open top chambers

NFCs:

Non-filtered chambers

FCs:

Filtered chambers

OPs:

Open plots

O3 :

Ozone

ppb:

Parts per billion

RNPK:

Recommended NPK

1.5 RNPK:

1.5 times recommended NPK

APX:

Ascorbate peroxidase

SOD:

Superoxide dismutase

POD:

Peroxidase

MDA:

Malondialdehyde

LPO:

Lipid peroxidation

AsA:

Ascorbic acid

ROS:

Reactive oxygen species

DAG:

Days after germination

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Acknowledgment

The authors are thankful to the Department of Science and Technology, Government of India, New Delhi for providing financial support to the work.

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  1. Laboratory of Air Pollution and Global Climate Change, Department of Botany, Banaras Hindu University, Varanasi, 221005, India

    Poonam Singh, Madhoolika Agrawal & Shashi Bhushan Agrawal

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  1. Poonam Singh
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  2. Madhoolika Agrawal
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Correspondence to Madhoolika Agrawal.

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Singh, P., Agrawal, M. & Agrawal, S.B. Differences in Ozone Sensitivity at Different NPK Levels of Three Tropical Varieties of Mustard (Brassica campestris L.): Photosynthetic Pigments, Metabolites, and Antioxidants. Water Air Soil Pollut 214, 435–450 (2011). https://doi.org/10.1007/s11270-010-0434-9

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