Abstract
Municipal wastewaters with industrial discharges typically contain heavy metals which may inhibit the biological processes in wastewater treatment plants. In this study, copper inhibition on strict nitrifiers in a suspended growth (SG) reactor and a combined attached and suspended growth (A&SG) reactor was compared. Both reactors were subjected to a continuous copper input of 5 mg/L. When the accumulated total copper concentration in the reactor were approximately 25 mg/L (due to sorption to the biomass), a sharp decrease in nitrification (increase in inhibition) were observed in the SG reactor while nitrification remained the same for the A&SG reactor indicating that attached growth systems were more robust against copper toxicity than suspended growth systems. Using MINTEQA2, the concentrations of various chemical species were estimated and, of the different species present, adsorbed copper in the biomass and aqueous Cu(NH3)4 +2 were found to positively correlate with percent inhibition of nitrification. Based on the changes in the concentrations of the two species, Cu(NH3)4 +2 was probably the main chemical species responsible for inhibition of nitrification. This study has implications for wastewater treatment plants treating wastewaters with high ammonia and copper present.
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References
APHA.AWWA, WEF (1998). Standard methods for the examination of water and wastewater (20th ed.). Washington, D.C.: American Public Health Association.
Benninger, R. W., & Sherrard, J. H. (1979). Nitrification and alkalinity relationships in activated sludge. Journal—Water Pollution Control Federation, 9, 2132–2142.
Braam, F., & Klapwijk, A. (1981). Effect of copper on nitrification in activated sludge. Water Research, 15, 1093–1098. doi:10.1016/0043-1354(81)90077-4.
Cabrero, A., Fernandez, S., Mirada, F., & Garcia, J. (1998). Effects of copper and zinc on the activated sludge bacteria growth kinetics. Water Research, 32, 1355–1362. doi:10.1016/S0043-1354(97)00366-7.
Chua, H., Yu, P., Sin, S., & Cheung, M. (1999). Sub-lethal effects of heavy metals on activated sludge micro-organisms. Chemosphere, 39, 2681–2692. doi:10.1016/S0045-6535(99)00203-9.
Gee, C. S., Suidan, M. T., & Pfeffer, J. T. (1990). Nitrosomonas and Nitrobacter interactions in biological nitrification. Journal of Environmental Engineering, 116, 4–17. doi:10.1061/(ASCE)0733-9372(1990)116:1(4).
Harrison, J., Ceri, H., Stremick, C., & Turner, R. (2004). Biofilm susceptibility to metal toxicity. Environmental Microbiology, 6, 1220–1227. doi:10.1111/j.1462-2920.2004.00656.x.
Harrison, J., Rabiei, M., Turner, R. J., Badry, E. A., Sproule, K. M., & Ceri, H. (2006). Metal resistance in Candida biofilms. FEMS Microbiology Ecology, 55, 479–491. doi:10.1111/j.1574-6941.2005.00045.x.
Hu, Z., Chandran, K., Grasso, D., & Smets, B. (2002). Effect of nickel and cadmium speciation on nitrification inhibition. Environmental Science & Technology, 36, 3074–3078. doi:10.1021/es015784a.
Hu, Z., Chandran, K., Grasso, D., & Smets, B. (2003). Impact of metal sorption and internalization on nitrification inhibition. Environmental Science & Technology, 37, 728–734. doi:10.1021/es025977d.
Hu, Z., Chandran, K., Grasso, D., & Smets, B. (2004). Comparison of nitrification inhibition by metals in batch and continuous flow reactors. Water Research, 38, 3949–3959. doi:10.1016/j.watres.2004.06.025.
Kim, K., Kim, I., Hwang, S., & Kim, S. (2006). Estimating the combined effects of copper and phenol to nitrifying bacteria in wastewater treatment plants. Water Research, 40, 561–568. doi:10.1016/j.watres.2005.12.020.
Lee, Y. W., Ong, S. K., & Sato, C. (1997). Effects of heavy metals on nitrifying bacteria. Water Science and Technology, 36, 67–74.
Nelson, P. O., Chung, A. K., & Hudson, M. C. (1981). Factors affecting the fate of heavy metals in the activated sludge process. Journal - Water Pollution Control Federation, 53, 1323–1333.
Neufeld, R. D., Hill, A. J., & Adekoya, D. O. (1980). Phenol and free ammonia Inhibition to Nitrosomonas Activity. Water Research, 14, 1695–1703. doi:10.1016/0043-1354(80)90105-0.
Sato, C., Schnoor, J. L., & McDonald, D. B. (1986a). Characterization of effects of copper cadmium, and nickel on the growth of Nitrosomonas europaea. Environmental Toxicology and Chemistry, 5, 403–416. doi:10.1897/1552-8618(1986)5[403:COEOCC]2.0.CO;2.
Sato, C., Schnoor, J. L., & McDonald, D. B. (1986b). Effects of copper and nickel on the growth of Nitrosomonas europaea. Toxicity assessment. Toxicity Assessment, 1, 357–376.
Sato, C., Leung, S. W., & Schnoor, J. L. (1988). Toxic response of Nitrosomonas europaea to copper in inorganic medium and wastewater. Water Research, 22, 1117–1127. doi:10.1016/0043-1354(88)90006-1.
Scullion, J., Winson, M., & Matthews, R. (2007). Inhibition and recovery in a fixed microbial film leachate treatment system subject to shock loading of copper and zinc. Water Research, 41, 4129–4138. doi:10.1016/j.watres.2007.05.043.
Semerci, N., & Çeçen, F. (2007). Importance of cadmium speciation in nitrification inhibition. Journal of Hazardous Materials, 17, 503–512. doi:10.1016/j.jhazmat.2007.01.041.
Sterritt, R. M., & Lester, J. N. (1980). Interaction of heavy metals with bacteria. The Science of the Total Environment, 14, 5–17. doi:10.1016/0048-9697(80)90122-9.
Teitzel, G., & Parsek, R. (2003). Heavy metal resistance of biofilm and planktonic Pseudomonas aeruginosa. Applied and Environmental Microbiology, 69, 2313–2320. doi:10.1128/AEM.69.4.2313-2320.2003.
US EPA (1998). MINTEQA2/PRODEFA2, A geochemical assessment model for environmental systems: User manual v. 4.0. Athens: US EPA.
Weon, S., Koopman, B., & Lee, I. (2004). Effects of toxicants on nitrifying biomass in flocs vs. carriers. Environmental Technology, 25, 193–199. doi:10.1080/09593330409355452.
Wood, L. B., Hurley, B. J. E., & Matthews, P. J. (1981). Some observations on the biochemistry and inhibition of nitrification. Water Research, 15, 543–551. doi:10.1016/0043-1354(81)90017-8.
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Lee, YW., Tian, Q., Ong, S.K. et al. Inhibitory Effects of Copper on Nitrifying Bacteria in Suspended and Attached Growth Reactors. Water Air Soil Pollut 203, 17–27 (2009). https://doi.org/10.1007/s11270-009-9988-9
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DOI: https://doi.org/10.1007/s11270-009-9988-9