Abstract
The concentration and size distribution of bacterial and fungal aerosol was studied in 15 houses. The houses were categorized into three types, based on occupant density and number of rooms: single room in shared accommodation (type I), single bedroom flat in three storey buildings (type II) and two or three bedroomed houses (type III). Sampling was undertaken with an Anderson six-stage impactor during the summer of 2007 in the living rooms of all the residential settings. The maximum mean geometric concentration of bacterial (5,036 CFU/m3, ± 2.5, n = 5) and fungal (2,124 CFU/m3, ± 1.38, n = 5) aerosol were in housing type III. The minimum levels of indoor culturable bacteria (1,557 CFU/m3, ±1.5, n = 5) and fungal (925 CFU/m3, ±2.9, n = 5) spores were observed in housing type I. The differences in terms of total bacterial and fungal concentration were less obvious between housing types I and II as compared to type III. With reference to size distribution, the dominant stages for culturable bacteria in housing types I, II and III were stage 3 (3.3–4.7 μm), stage 1 (7 μm and above) and stage 5 (1.1–2.1 μm), respectively. Whereas the maximum numbers of culturable fungal spores were recovered from stage 2 (4.7–7 µm), in housing type I, and from stage 4 (2.1–3.3 μm) in both type II and III houses. The average geometric mean diameter of bacterial aerosol was largest in type I (4.7 μm), followed by type II (3.89 μm) and III (1.96 μm). Similarly, for fungal spores, type I houses had the highest average mean geometric diameter (4.5 μm), while in types II and III the mean geometric diameter was 3.57 and 3.92 μm, respectively. The results indicate a wide variation in total concentration and size of bioaerosols among different residential settings. The observed differences in the size distributions and concentrations reflect their variable airborne behaviour and, as a result, different risks of respiratory exposure of the occupants to bioaerosols in various residential settings.
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References
ACGIH. (1989). Guidelines for the assessment of bioaerosols in the indoor environment. Cincinnati: American Conference of Governmental Industrial Hygienists.
ACGIH. (1999). Bioaerosols: Assessment and control. Cincinnati: American Conference of Governmental Industrial Hygienists.
Awad, A. H. A. (2007). Airborne dust, bacteria, actinomycetes and fungi at a flourmill. Aerobiologia, 23, 59–69.
Aydogdu, H., Asan, A., Otkun, M. T., & Ture, M. (2005). Monitoring of fungi and bacteria in the indoor air of primary schools in Edirne city, Turkey. Indoor and Built Environment, 14, 411–425.
Buttner, M. P., & Stetzenbach, L. D. (1993). Monitoring airborne fungal fpores in an experimental indoor environment to evaluate sampling methods and the effects of human activity on air sampling. Applied and Environmental Microbiology, 59, 219–226.
DeKoster, J. A., & Thorne, P. S. (1995). Bioaerosol concentrations in noncomplaint, complaint, and intervention homes in the Midwest. American Industrial Hygiene Association Journal, 56, 573–580.
Desai, M. R., & Ghosh, S. K. (2003). Occupational exposure to airborne fungi among rice mill workers with special reference to aflatoxin producing A-flavus strains. Annals of Agricultural and Environmental Medicine, 10, 159–162.
Douwes, J., Thorne, P., Pearce, N., & Heederik, D. (2003). Bioaerosol health effects and exposure assessment: progress and prospects. Annals of Occupational Hygiene, 47, 187–200.
Dowd, S. E., & Maier, R. M. (2000). Aeromicrobiology. In R. M. Maier, I. L. Pepper, & C. P. Gerba (Eds.), Environmetal microbiology (pp. 91–122). San Diego: Academic.
Ellis, J. J. (1981). The effect of medium temperature and age on Rhizopus delemar sporangiospore size. Mycologia, 73, 362–368.
Environment Canada (1989). Exposure guidelines for residential indoor air quality (p. 23). Ottawa: Federal-Provincial Advisory Committee on Environmental and Occupational Health.
Foarde, K., Dulaney, P., Cole, E., VanOsdel, D., Ensor, D., Chang, J. (1993) Assessment of fungal growth on ceiling tiles under environmentally characterized conditions. In P. Kalliokoski, M. Jantunen, O. Seppänen (Eds.) Proceedings of Indoor Air 4:357–362
Gorny, R. L., Dutkiewicz, J., & Krysiska-Traczyk, E. (1999). Size distribution of bacterial and fungal bioaerosols in indoor air. Annals of Agricultural and Environmental Medicine, 6, 105–113.
Green, C. F., Scarpino, P. V., & Gibbs, G. (2003). Assessemnt and modeling of indoor fungal and bacterial bioaerosol concentration. Aerobiologia, 19, 159–169.
Haas, D., Habib, J., Galler, H., Buzina, W., Schlacher, R., Marth, E., et al. (2007). Assessment of indoor air in Austrian apartments with and without visible mold growth. Atmospheric Environment, 41, 5192–5201.
Hameed, A. A. A., Shakour, A. A., & Yasser, H. I. (2003). Evaluation of bio-aerosols at an animal feed manufacturing industry: a case study. Aerobiologia, 19, 1573–3025.
Hunter, C. A., Grant, C., Flannigan, B., & Bravery, A. F. (1988). Mould in buildings: the air spora of domestic dwellings. International Biodeterioration and Biodegradation, 24, 81–101.
Hunter, C. A., Hull, A. V., Higham, D. F., Grimes, C. P., & Lea, R. G. (1996). Fungi and bacteria. In R. W. Berry, V. M. Brown, S. K. D. Coward, D. R. Crump, M. Gavin, C. P. Grimes, D. F. Higham, A. V. Hull, C. A. Hunter, I. G. Jeffery, R. G. Lea, J. W. Llewellyn, & G. J. Raw (Eds.), Indoor air quality in homes: part 1 (pp. 97–115). Gartson: Building Research Establishment.
Hyvarinen, A., Vahteristo, M., Meklin, T., Jantunen, M., Nevalainen, A., & Moschandreas, D. (2001). Temporal and spatial variation of fungal concentrations in indoor air. Aerosol Science and Technology, 35, 688–695.
Jain, A. K. (2000). Survey of bioaerosol in different indoor working environments in central India. Aerobiologia, 16, 221–225.
Jones, A. M., & Harrison, R. M. (2004). The effects of meteorological factors on atmospheric bioaerosol concentrations—A review. Science of the Total Environment, 326, 151–180.
Jothish, P. S., & Nayar, T. S. (2004). Airborne fungal spores in a sawmill environment in Palakkad District, Kerala, India. Aerobiologia, 20, 75–81.
Kim, K. Y., & Kim, C. N. (2007). Airborne microbiological characteristics in public buildings of Korea. Building and Environment, 42, 2188–2196.
Kulmala, M., Asmi, A., & Pirjola, L. (1999). Indoor air aerosol model: The effect of outdoor air, filtration and ventilation on indoor concentrations. Atmospheric Environment, 33, 2133–2144.
Lee, J. H., & Jo, W. K. (2006). Characteristics of indoor and outdoor bioaerosols at Korean high-rise apartment buildings. Environmental Research, 101, 11–17.
Li, C. S., & Kuo, T. M. (1993). Microbiological indoor air quality in subtropical areas. Environment International, 19, 233–239.
Liao, C. M., Luo, W. C., Chen, S. C., Chen, J. W., & Liang, H. M. (2004). Temporal/seasonal variations of size-dependent airborne fungi indoor/outdoor relationships for a wind-induced naturally ventilated airspace. Atmospheric Environment, 38, 4415–4419.
Lin, W. H., & Li, C. S. (1996). Size characteristics of fungus allergens in the subtropical climate. Aerosol Science and Technology, 25, 93–100.
Meklin, T., Reponen, T., Toivola, M., Koponen, V., Husman, T., Hyvarinen, A., et al. (2002). Size distributions of airborne microbes in moisture-damaged and reference school buildings of two construction types. Atmospheric Environment, 36, 6031–6039.
Mohr, A. J. (2001). Fate and transport of microorganisms in air. In C. J. Hurst, R. L. Crawford, G. R. Knudson, M. J. McInerney, & L. D. Stetzenbach (Eds.), Manual of environmental microbiology (2nd ed., pp. 827–838). Washington: ASM.
Moschandreas, D. J., Pagilla, K. R., & Storino, L. V. (2003). Time and space uniformity of indoor bacteria concentrations in Chicago area residences. Aerosol Science and Technology, 37, 899–906.
Musk, A. W., Venables, K. M., Crook, B., Nunn, A. J., Hawkins, R., Crook, G. D. W., et al. (1989). Respiratory symptoms, lung-function, and sensitization to flour in a British bakery. British Journal of Industrial Medicine, 46, 636–642.
Narayan, M. C. J., Ravichandran, V., & Sullia, S. B. (1982). Aeromycology of the atmosphere of Malleeswaram Market, Bangalore. Acta Botanica Indica, 10, 196–200.
Oppliger, A., Rusca, S., Charriere, N., Duc, T. V., & Droz, P. O. (2005). Assessment of bioaerosols and inhalable dust exposure in Swiss sawmills. Annals of Occupational Hygiene, 49, 385–391.
Pasanen, A. L., Pasanen, P., Jantunen, M. J., & Kalliokoski, P. (1991). Significance of air humidity and air velocity for fungal spore release into the air. Atmospheric Environment Part A—General Topics, 25, 459–462.
Pasanen, A. L., Kasanen, J. P., Rautiala, S., Ikaheimo, M., Rantamaki, J., Kaariainen, H., et al. (2000). Fungal growth and survival in building materials under fluctuating moisture and temperature conditions. International Biodeterioration and Biodegradation, 46, 117–127.
Pastuszka, J. S., Paw, U. K. T., Lis, D. O., Wlazlo, A., & Ulfig, K. (2000). Bacterial and fungal aerosol in indoor environment in Upper Silesia, Poland. Atmospheric Environment, 34, 3833–3842.
Ren, P., Jankun, T. M., & Leaderer, B. P. (1999). Comparisons of seasonal fungal prevalence in indoor and outdoor air and in house dusts of dwellings in one Northeast American county. Journal of Exposure Analysis and Environmental Epidemiology, 9, 560–568.
Ren, P., Jankun, T. M., Belanger, K., Bracken, M. B., & Leaderer, B. P. (2001). The relation between fungal propagules in indoor air and home characteristics. Allergy, 56, 419–424.
Reponen, T., Hyvarinen, A., Ruuskanen, J., Raunemaa, T., & Nevalainen, A. (1994). Comparison of concentrations and size distributions of fungal spores in buildings with and without mold problems. Journal of Aerosol Science, 25, 1595–1603.
Reponen, T., Willeke, K., Ulevicius, V., Reponen, A., & Grinshpun, S. A. (1996). Effect of relative humidity on the aerodynamic diameter and respiratory deposition of fungal spores. Atmospheric Environment, 30, 3967–3974.
Ritschkoff AC, Viitanen H, Koskela K (2000) The response of building materials to the mould exposure at different humidity and temperature conditions. Proceedings of Healthy Buildings, Espoo, August 6–10, 1, pp 317–322
Rolka, H., Krajewska-Kulak, E., Lukaszuk, C., Oksiejczuk, E., Jakoniuk, P., Leszczynska, K., et al. (2005). Indoor air studies of fungi contamination of social welfare home in Czerewki in north-east part of Poland. Roczniki Akademii Medycznej w Bialymstoku, 50(Suppl 1), 26–30.
Rosas, I., Calderon, C., Salinas, E., Martınez, L., Alfaro-Moreno, E., Milton, D. K., et al. (2001). Animal and worker exposure to dust and biological particles in animal care houses. Aerobiologia, 17, 49–59.
Savino, E., & Caretta, G. (1992). Airborne fungi in an Italian rice mill. Aerobiologia, 8, 267–275.
Shelton, B. G., Kirkland, K. H., Flanders, W. D., & Morris, G. K. (2002). Profiles of airborne fungi in buildings and outdoor environments in the United States. Applied and Environmental Microbiology, 68, 1743–1753.
Singh, A., & Singh, A. B. (1994). Airborne fungi in a bakery and the prevalence of respiratory dysfunction among workers. Grana, 33, 349–358.
Strachan, D. P., Flannigan, B., McCabe, E. M., & McGarry, F. (1990). Quantification of airborne molds in the homes of children with and without wheeze. Thorax, 45, 382–387.
Tsai, F. C., & Macher, J. M. (2005). Concentrations of airborne culturable bacteria in 100 large US office buildings from the BASE study. Indoor Air, 15, 71–81.
Viitanen H, Hanhijärvi A, Hukka A, Koskela K (2000) Modelling mould growth and decay damages. Proceedings of Healthy Buildings, Espoo, August 6–10, 3:317–322
WHO (2007) Development of WHO guidelines for indoor air quality: dampness and mould, report on a working group meeting Bonn, Germany, 17–18 October
Zorman, T., & Jersek, B. (2008). Assessment of bioaerosol concentrations in different indoor environments. Indoor and Built Environment, 17, 155–163.
Zuraimi, M. S., & Tham, K. W. (2008). Indoor air quality and its determinants in tropical child care centers. Atmospheric Environment, 42, 2225–2239.
Zuraimi, M. S., Fang, L., Tan, T. K., Chew, F. T., & Tham, K. W. (2009). Airborne fungi in low and high allergic prevalence child care centers. Atmospheric Environment, 43, 2391–2400.
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Nasir, Z.A., Colbeck, I. Assessment of Bacterial and Fungal Aerosol in Different Residential Settings. Water Air Soil Pollut 211, 367–377 (2010). https://doi.org/10.1007/s11270-009-0306-3
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DOI: https://doi.org/10.1007/s11270-009-0306-3