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Metal Availability and Chemical Properties in the Rhizosphere of Lupinus albus L. Growing in a High-Metal Calcareous Soil

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Abstract

Chemical processes in the rhizosphere play a major role in the availability of metals to plants. The objective of this study was to assess the potential of white lupin (Lupinus albus L.) for the phytoimmobilisation of heavy metals in a calcareous soil with high levels of Zn and Pb (2,058 and 2,947 μg g−1, respectively) by evaluating the chemical changes in the rhizosphere, relative to bulk soil, which modify the solubility of heavy metals. Plants were cultivated for 74 days in specially designed pots (rhizopots) in which rhizosphere was sampled easily under controlled conditions. White lupin accumulated high concentrations of Mn in the shoots (average of 4,960 μg g−1), well above the normal concentration in plants (300 μg g−1). But the metal concentrations found in shoots were not at toxic levels. Rhizosphere soil showed a significantly greater redox potential (245 mV) and water-soluble organic carbon content (34.6 μg C g−1) than bulk soil (227 mV; 27.6 μg C g−1). Root activity decreased EDTA-extractable Pb, Zn and Fe and promoted their precipitation as insoluble compounds in the residual fraction (acid digestion), hardly available to plants. These results indicate the suitability of this annual N2-fixing species for the initial phytoimmobilisation of heavy metals in contaminated soils.

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Acknowledgements

This work was financed by the Ministry of Education and Science (ref.: CTM2004-06715-C02-02) and the Fundación Séneca (ref.: 00571/PI/04). The authors thank the Ministry of Education and Science for the FPI grant awarded to Isabel Martínez Alcalá.

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  1. Department of Soil and Water Conservation and Organic Waste Management, Centro de Edafología y Biología Aplicada del Segura, CSIC, Campus Universitario de Espinardo, Apartado 164, 30100, Murcia, Spain

    Isabel Martínez-Alcalá, Rafael Clemente & M. Pilar Bernal

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  1. Isabel Martínez-Alcalá
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  2. Rafael Clemente
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  3. M. Pilar Bernal
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Correspondence to M. Pilar Bernal.

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Martínez-Alcalá, I., Clemente, R. & Bernal, M.P. Metal Availability and Chemical Properties in the Rhizosphere of Lupinus albus L. Growing in a High-Metal Calcareous Soil. Water Air Soil Pollut 201, 283–293 (2009). https://doi.org/10.1007/s11270-008-9944-0

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