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Evolution of Bacterial Community in Experimental Sand Filters: Physiological and Molecular Fingerprints

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Abstract

Biofilm development in wastewater treatment system by soil infiltration is often mentioned for its participation to purification efficiency and clogging zone formation. It appears necessary to understand its evolution in order to better control the operation of these systems. The objective of this study was to improve knowledge about the temporal evolution of the biofilm structure in the first centimetres of infiltration system. For this purpose, metabolic fingerprints by Biolog EcoPlate™ and molecular fingerprints by Ribosomal Intergenic Spacer Analysis (RISA) were carried out on sand, septic effluent and treated effluent samples from two experimental reactors supplied with different hydraulic loads collected at different times. The metabolic capabilities of sand microflora decreased in time. In the same way, molecular structure of the biofilm community changed and converged to similar structure in time. Principal components analysis on RISA gel revealed a “buffering effect” of the sand filter on the genetic structure of the bacterial community from treated effluent. The kinetics of evolution of the both metabolic and genetic fingerprints showed a reduction of the metabolic and genetic potentials of septic and treated effluents for the same times. The population dynamic within the biofilms appears interesting evidence in the comprehension of the operation of the treatment systems.

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Authors and Affiliations

  1. Département Climatologie Aérodynamique Pollution et Epuration, Centre Scientifique et Technique du Bâtiment, 11 rue Henri Picherit, BP 82341, 44323, Nantes cedex 03, France

    Sylvaine Chabaud & Abdel Lakel

  2. Microbiologie du Sol et de l’Environnement CMSE-INRA, UMR INRA 1229/Université de Bourgogne, 17 rue Sully, BP 86510, 21065, Dijon Cedex, France

    Fabrice Martin-Laurent

  3. Ecole des Mines de Nantes, GEPEA UMR CNRS 6144, 4 rue Alfred Kastler, BP 20722, 44307, Nantes cedex 03, France

    Yves Andres

  4. Sciences Chimiques de Rennes, Ecole de Chimie de Rennes (ENSCR) UMR CNRS 6226, Campus de Beaulieu, 263 Avenue du Général Leclerc, 35700, Rennes, France

    Pierre Le Cloirec

Authors
  1. Sylvaine Chabaud
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  2. Fabrice Martin-Laurent
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  3. Yves Andres
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  4. Abdel Lakel
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  5. Pierre Le Cloirec
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Correspondence to Yves Andres.

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Chabaud, S., Martin-Laurent, F., Andres, Y. et al. Evolution of Bacterial Community in Experimental Sand Filters: Physiological and Molecular Fingerprints. Water Air Soil Pollut 195, 233–241 (2008). https://doi.org/10.1007/s11270-008-9742-8

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  • DOI: https://doi.org/10.1007/s11270-008-9742-8

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