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Study on the regulatory mechanism of latent membrane protein 2A on GCNT3 expression in nasopharyngeal carcinoma

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Abstract

O-Glycan synthesis enzyme glucosaminyl (N-acetyl) transferase 3 (GCNT3) is closely related to the occurrence and development of various cancers. However, the regulatory mechanism and function of GCNT3 in nasopharyngeal carcinoma (NPC) are still poorly understood. This study aims to explore the regulatory mechanism of EBV-encoded latent membrane protein 2A (LMP2A) on GCNT3 and the biological role of GCNT3 in NPC. The results show that LMP2A can activate GCNT3 through the mTORC1 pathway, and there is a positive feedback between the mTORC1 and GCNT3. GCNT3 regulates EMT progression by forming a complex with ZEB1 to promote cell migration. GCNT3 can also promote cell proliferation. These findings indicate that targeting the LMP2A-mTORC1-GCNT3 axis may represent a novel therapeutic target in NPC.

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Funding

Funding was provided by Natural Science Foundation of Shandong Province (Grant Nos.: ZR2020MC020, ZR2021MC068, ZR2020MH302).

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Authors and Affiliations

  1. Department of Pathogenic Biology, School of Basic Medicine, Qingdao University, Qingdao, China

    Yijing Chen, Yan Zhang, Shi Duo, Wen Liu & Bing Luo

  2. Department of Clinical Laboratory, Central Hospital of Zibo, Zibo, China

    Yan Zhang

  3. Department of Clinical Laboratory, The Sixth Hospital of Wuhan, Affiliated Hospital of Jianghan University, Wuhan, China

    Yijing Chen

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  1. Yijing Chen
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  2. Yan Zhang
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  3. Shi Duo
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  4. Wen Liu
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  5. Bing Luo
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Contributions

YC, DS, YZ, WL and BL designed research; YC performed research; YC and WL analyzed data; YC prepared original draft; WL and BL reviewed and edited the manuscript. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Wen Liu or Bing Luo.

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Edited by Joachim J. Bugert.

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Chen, Y., Zhang, Y., Duo, S. et al. Study on the regulatory mechanism of latent membrane protein 2A on GCNT3 expression in nasopharyngeal carcinoma. Virus Genes (2024). https://doi.org/10.1007/s11262-024-02071-w

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