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Identification of monoclonal antibody targeting epitope on p72 trimeric spike of African swine fever virus

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Abstract

African swine fever virus (ASFV) is highly contagious and can cause lethal disease in pigs. ASFV p72 protein is a major capsid protein that presents as trimer in the virion. Epitopes on the surface of p72 trimer are considered as protective antigens. In this study, recombinant p72 protein and p72-baculovirus were constructed and obtained. Three monoclonal antibodies (mAbs) specific to ASFV p72 protein, designated as 1A3, 2B5 and 4A5, were generated. Among them, 4A5 showed strong reactivity with ASFV infected cells. Subsequently, the epitope recognized by 4A5 was mapped and identified using a series of overlapping peptides generated from p72 protein. IFA and western blot analyses showed that 4A5 recognized the linear epitope of p72 monomer located between amino acids 245–285 and recognized the conformational epitope located at the surface and top of the p72 trimer. These findings will enrich our knowledge regarding the epitope on p72 protein and provide valuable information for further characterization of the antigenicity and molecular functions of p72 protein.

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Data Availability

The data generated or analyzed during this study are included in this published article.

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Acknowledgements

This research work was funded by the National Key Research and Development Program of China (2021YFD1801300 and 2021YFD1801401), National Nature Science Foundation (32170161) and Central Public-interest Scientific Institution Basal Research Fund (Y2022PT11). The authors would like to thank these.

Funding

This research work was funded by the National Key Research and Development Program of China (2021YFD1801300 and 2021YFD1801401), National Nature Science Foundation (32170161) and Central Public-interest Scientific Institution Basal Research Fund (Y2022PT11).

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Authors and Affiliations

  1. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China

    Xulai Duan, Yingnan Liu, Zongyan Chen, Zhenhua Xie, Chuanwen Tian, Yao Li, Lu Lv, Rongrong Wang, Jingyi Liu & Hongjun Chen

  2. Biosafety Research Center, Chinese Academy of Agricultural Sciences (CAAS), Shanghai, China

    Yingnan Liu, Zongyan Chen, Jingyi Liu & Hongjun Chen

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  1. Xulai Duan
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Contributions

The project was conceived and designed by HC. XD, YL, ZX et al. performed experiments. HC, JL, ZC and XD analyzed the data. JL and HC wrote the paper. HC supervised all the experiments. All authors contributed to the article and approved the submitted version.

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Correspondence to Jingyi Liu or Hongjun Chen.

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Duan, X., Liu, Y., Chen, Z. et al. Identification of monoclonal antibody targeting epitope on p72 trimeric spike of African swine fever virus. Virus Genes 59, 582–590 (2023). https://doi.org/10.1007/s11262-023-02003-0

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