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Characterization of the first Rubus yellow net virus genome from blackberry

Abstract

Rubus yellow net virus (RYNV) is a badnavirus that infects Rubus spp. Mixed infections with black raspberry necrosis virus and raspberry leaf mottle virus cause raspberry mosaic, a disease that leads to significant losses and even plant death. RYNV has been reported in several European countries and the Americas yet there is substantial lack of knowledge, especially when it comes to virus diversity and the evolutionary forces that affect virus fitness outside its primary host, raspberry. Herein, we report the first RYNV episomal genome isolated from blackberry and this is the first report of the virus in Bosnia and Herzegovina. The isolate has five open reading frames (ORFs) and, when compared with other fully sequenced counterparts, showed 82–97% nucleotide pairwise identity. This communication adds to our limited knowledge on RYNV and addresses some of the gaps in RYNV genetics when it comes to the coding capacity of episomal isolates and the probability of the first fully sequenced isolate of the virus being integrated in the raspberry genome.

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Fig. 1

Data availability

The nucleotide sequence of the virus reported in this work has been deposited in the GenBank database under accession number MZ358192.

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Acknowledgements

The authors acknowledge the Fulbright program for Duška Delić's sabbatical at the University of Arkansas.

Funding

This work was partially funded by the Arkansas Agricultural Experimental Station and USDA-NIFA Hatch project 1002361.

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Contributions

DS, DD, and IET contributed to study conception and design. Material preparation, data collection, and analysis were performed by MV, DS, and DD. The first draft of the manuscript was written by all authors; and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Ioannis E. Tzanetakis.

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The authors declare no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Edited by Seung-Kook Choi.

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Vakić, M., Stainton, D., Delić , D. et al. Characterization of the first Rubus yellow net virus genome from blackberry. Virus Genes (2022). https://doi.org/10.1007/s11262-022-01926-4

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  • DOI: https://doi.org/10.1007/s11262-022-01926-4

Keywords

  • Badnavirus
  • Episomal
  • Rubus fruticosus L
  • Evolutionary analysis