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Isolation and characterization of the novel bacteriophage vB_SmaS_BUCT626 against Stenotrophomonas maltophilia

Abstract

Stenotrophomonas maltophilia has been recognized as an emerging global opportunistic pathogen, and it is intrinsically resistant to most antibiotics, which makes the limited choice for treating S. maltophilia infections. Bacteriophage with the proper characterization is considered as a promising alternative treatment option to control S. maltophilia infections. In this study, we isolated a novel Siphoviridae bacteriophage vB_SmaS_BUCT626 with lytic activity against S. maltophilia. Phage vB_SmaS_BUCT626 can lysis 10 of 20 S. maltophilia and was relatively stable at a wide range of temperatures (4–70 °C) and pH values (3.0–13.0) and exhibited good tolerance to chloroform. The genome of phage vB_SmaS_BUCT626 was a 61,662-bp linear double-stranded DNA molecule with a GC content of 56.2%, and contained 100 open-reading frames. It carried no antibiotic resistance, toxin, virulence-related genes, or lysogen-formation gene clusters. Together, these characteristics make phage vB_SmaS_BUCT626, a viable candidate as a biocontrol agent against S. maltophilia infection.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (82002207), the Natural Science Foundation of Shanxi Province (201901D211136), Shanxi Province Science Foundation for Youths (201801D221285), National Key Research and Development Program of China (2018YFA0903000), and Technology Research and Development Program of Taian (2018NS0140).

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Authors and Affiliations

Authors

Contributions

YT, JF, XC, and WZ conceived and designed experiments and critically evaluated the manuscript. FL, LL, YZ, and JF drafted the manuscript, LF extracted the phage nucleotide and conducted the sequencing experiments. FL and YZ isolated and identified the phage and conducted the biological characterization experiments. SB, LS, and JG were involved in the phage bio-informatics analysis. However, the sequence submission author FT was not part of the work. All authors checked and reviewed the manuscript.

Corresponding authors

Correspondence to Wangmeng Zhang, Xiaogang Cui, Jiao Feng or Yigang Tong.

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Li, F., Li, L., Zhang, Y. et al. Isolation and characterization of the novel bacteriophage vB_SmaS_BUCT626 against Stenotrophomonas maltophilia. Virus Genes 58, 458–466 (2022). https://doi.org/10.1007/s11262-022-01917-5

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  • DOI: https://doi.org/10.1007/s11262-022-01917-5

Keywords

  • Stenotrophomonas maltophilia
  • Bacteriophage
  • Genome sequencing
  • Sequence analysis