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Interaction of influenza A virus NS1 and cytoskeleton scaffolding protein α-actinin 4

Virus Genes volume 58, pages 15–22 (2022)Cite this article

Abstract

NS1 (Non-structural protein 1) is a non-structural protein that can highly express when the avian influenza virus infects the host cells. NS1 can interact with various proteins to alter the intracellular distribution of host proteins and regulate the virulence and pathogenicity of the avian influenza virus. To further study the role of NS1 protein in replication and pathogenesis of avian influenza virus, Glutathione S-transferase (GST) Pull-down was used for screening more proteins interacting with NS1 in human lung adenocarcinoma cell line A549. By mass spectrometry, a potential interacted protein is identified as α-actinin 4 and its interaction with NS1 has not been reported yet. The interaction between NS1 and α-actinin 4 in vitro was confirmed by enzyme-linked immunosorbent assay experiments, and the results showed that the absorbance value of OD450nm in the experimental group was positively correlated with the concentration of NS1-GST protein compared to the negative control group. The co-immunoprecipitation and immunofluorescence results further confirmed the interaction between NS1 and α-actinin 4 at the cellular level. The interaction between NS1 and α-actinin 4 provided a new target for pathogenic mechanism studying and drug screening.

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Authors and Affiliations

  1. School of Life Sciences, Liaoning University, Shenyang, 110036, China

    Yingyue Zeng, Rui Wang, Fengchao Wang, Man Zhang, Li Zhang, Chunyu Zhu & Jian Zhao

  2. School of Pharmacy, Liaoning University, Shenyang, 110036, China

    Hongsheng Liu

  3. Engineering Laboratory of Molecular Modeling and Design for Drug of Liaoning Province, Shenyang, 110036, China

    Yingyue Zeng, Li Zhang, Jian Zhao & Hongsheng Liu

  4. Research Center for Computer Simulating and Information Processing of Bio-Macromolecules of Liaoning, Shenyang, 110036, China

    Yingyue Zeng, Li Zhang, Chunyu Zhu, Jian Zhao & Hongsheng Liu

  5. Technology Innovation Center for Computer Simulating and Information Processing of Bio-Macromolecules of Shenyang, Shenyang, 110036, China

    Yingyue Zeng, Li Zhang, Jian Zhao & Hongsheng Liu

Authors
  1. Yingyue Zeng
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  2. Rui Wang
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  4. Man Zhang
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  5. Li Zhang
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  7. Jian Zhao
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  8. Hongsheng Liu
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Correspondence to Hongsheng Liu.

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Zeng, Y., Wang, R., Wang, F. et al. Interaction of influenza A virus NS1 and cytoskeleton scaffolding protein α-actinin 4. Virus Genes 58, 15–22 (2022). https://doi.org/10.1007/s11262-021-01876-3

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  • DOI: https://doi.org/10.1007/s11262-021-01876-3

Keywords

  • Avian influenza virus
  • NS1
  • α-Actinin 4
  • Interaction
  • Pull-down