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Complete genome sequences of novel Berlinvirus and novel Certrevirus lytic for Pectobacterium sp. causing soft rot and black leg disease of potato

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Abstract

Two novel dsDNA bacteriophages named Pectobacterium virus CB251 (PcCB251) and Pectobacterium virus CB7V (PcCB7V) targeting plant pathogen Pectobacterium parmentieri have been isolated and sequenced. The PcCB251 genome consists of 40,557 bp with G+C content of 48.6% and contains 47 predicted genes on a single strand. The phage is classified in genus Berlinvirus, family Autographiviridae. The PcCB7V phage has a circular dsDNA genome of 146,054 bp with G+C content of 50.4% and contains 269 predicted protein genes on both strands and 13 tRNA genes. The PcCB7V phage can be classified in genus Certrevirus, subfamily Vequintavirinae. Both novel bacteriophages have narrow host ranges, but they extend the list of candidates for phage-based control of pectolytic bacteria causing soft rot disease of potato.

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Acknowledgements

This research work was supported by project QK1910028 from the Ministry of Agriculture of the Czech Republic and by institutional funding RVO60077344.

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Authors and Affiliations

  1. Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Czech Academy of Sciences, Branišovská 31, České Budějovice, Czech Republic

    Karel Petrzik & Sára Brázdová

  2. Potato Research Institute Havlíčkův Brod, Dobrovského 2366, Havlíčkův Brod, 580 01, Czech Republic

    Martin Kmoch

  3. University of Chemistry and Technology Prague, Technická 1905/5, Prague 6-Dejvice, Prague, Czech Republic

    Rudolf Ševčík

Authors
  1. Karel Petrzik
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  2. Martin Kmoch
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  3. Sára Brázdová
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  4. Rudolf Ševčík
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Contributions

All authors contributed to the study’s conception and design. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Karel Petrzik.

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The authors declare no conflict of interest. The funding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

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As only soil and plant samples were used, no approval from the ethical committee was needed.

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This work does not contain any animal or human participants.

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Edited by Andrew Millard.

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Petrzik, K., Kmoch, M., Brázdová, S. et al. Complete genome sequences of novel Berlinvirus and novel Certrevirus lytic for Pectobacterium sp. causing soft rot and black leg disease of potato. Virus Genes 57, 302–305 (2021). https://doi.org/10.1007/s11262-021-01838-9

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