Molecular characterization of poleroviruses isolated from oilseed rape in Greece


In 2018 virus-like symptoms, typical of polerovirus infection were observed in several oilseed rape crops in northern Greece. In order to identify the etiological agent of these symptoms a polerovirus-generic RT-PCR assay was applied. Sequencing of the amplicons revealed the presence of virus isolates genetically close to turnip yellows virus (TuYV). Further molecular characterization of the near complete genome of ‘1–2’, ‘Geo1’, ‘Geo7’ and ‘Geo15’ isolates revealed that they share > 96% nt identity with various TuYV sequences. On the other hand, the fifth, characterized isolate from oilseed rape, termed ‘1–1’, showed higher sequence similarity to brassica yellows virus (BrYV) regarding the 5′ part of the complete coding sequence, whereas the 3′ part was closely related to TuYV isolates. A recombination analysis using RDP indicated the presence of a putative breakpoint (nucleotide position 2964) in ‘1–1’ genome and it is proposed that the virus isolate ‘1–1’ might be an interspecies recombinant between BrYV and TuYV. To our knowledge, this is the first time that the complete coding sequences of Greek TuYV isolates have been determined and the first detection of a BrYV/TuYV recombinant isolate infecting oilseed rape in Greece.

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Fig. 1


  1. 1.

    Tsialtas JT, Papantoniou AN, Baxevanos D, Papadopoulos II, Karaivazoglou N, Maslaris N, Papakosta DK (2017) Determinants of yield and quality in winter rapeseed (Brassica napus L.) under Mediterranean conditions. J Agr Sci 155:1577–1593

    CAS  Article  Google Scholar 

  2. 2.

    Latham LJ, Smith LJ, Jones RAC (2003) Incidence of three viruses in vegetable brassica plantings and associated wild radish weeds in south-west Australia. Australas Plant Pathol 32:387–391

    Article  Google Scholar 

  3. 3.

    Shahraeen N, Farzadfar S, Lesemann D-E (2003) Incidence of viruses infecting winter oilseed rape (Brassica napus ssp oleifera) in Iran. J Phytopathol 151:614–616

    Article  Google Scholar 

  4. 4.

    Schliephake E, Graichen K, Rabenstein F (2000) Investigations on the vector transmission of the Beet mild yellowing virus (BMYV) and the Turnip yellows virus (TuYV). J Plant Dis Prot 107:81–87

    Google Scholar 

  5. 5.

    Graichen K, Rabenstein F (1996) European isolates of Beet western yellows virus (BWYV) from oilseed rape (Brassica napus L. ssp napus) are non-pathogenic on sugar beet (Beta vulgaris L var altissima) but represent isolates of Turnip yellow virus (TuYV). J Plant Dis Prot 103:233–245

    Google Scholar 

  6. 6.

    Xiang H-Y, Dong S-W, Shang Q-X, Zhou C-J, Li D-W, Yu J-L, Han C-G (2011) Molecular characterization of two genotypes of a new polerovirus infecting brassicas in China. Arch Virol 156:2251–2255

    CAS  Article  Google Scholar 

  7. 7.

    New S-A, Van Heerden AW, Pietersen G, Esterhuizen LL (2016) First report of a Turnip yellows virus in association with the brassica stunting disorder in South Africa. Plant Dis 100:2341

    Article  Google Scholar 

  8. 8.

    Milošević D, Marjanović-Jeromela A, Ignjatov M, Jovičić D, Stanković I, Bulajić A, Krstić B (2015) First report of Turnip yellows virus on oilseed rape in Serbia. Plant Dis 99:1869

    Article  Google Scholar 

  9. 9.

    Veerakone S, Tang J, Zheng A, Ward LI (2018) First report of Turnip yellows virus in Daphne odora in New Zealand. Plant Dis 102:1467

    Article  Google Scholar 

  10. 10.

    Gaafar YZA, Ziebell H (2019) Two divergent isolates of Turnip yellows virus from pea and rapeseed and first report of Turnip yellows virus-associated RNA in Germany. Microbiol Resour Announce 8:e00214-e219.

    Article  Google Scholar 

  11. 11.

    Stevens M, McGrann G, Clark B, Authority H (2008) Turnip yellows virus (syn Beet western yellows virus): an emerging threat to European oilseed rape production? HGCA Res Rev 69:1–37

    Google Scholar 

  12. 12.

    Graichen K, Peterka H (1999) Generation of oilseed rape with resistance to Turnip yellows luteovirus. In: Wratten N, Salisbury PA (eds) 10th International rapeseed congress. International Consultative Group for Rapeseed Research. ACT, Canberra, pp 1–13

    Google Scholar 

  13. 13.

    Jones RAC, Coutts BA, Hawkes J (2007) Yield-limiting potential of Beet western yellows virus in Brassica napus. Aust J Agric Res 58:788–801

    Article  Google Scholar 

  14. 14.

    D’Arcy CJ, Domier LL (2005) Luteoviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Virus taxonomy, VIIIth Report of the ICTV. Elsevier/Academic Press, London, pp 891–900

    Google Scholar 

  15. 15.

    Zhang X-Y, Xiang H-Y, Zhou C-J, Li D-W, Yu J-L, Han C-G (2014) Complete genome sequence analysis identifies a new genotype of Brassica yellows virus that infects cabbage and radish in China. Arch Virol 159:2177–2180

    CAS  Article  Google Scholar 

  16. 16.

    Smirnova E, Firth AE, Miller WA, Scheidecker D, Brault V, Rainbold C, Rakotondrafara AM, Chung BT-W, Ziegler-Graff V (2015) Discovery of a small non-AUG-initiated ORF in poleroviruses and luteoviruses that is required for long-distance movement. PLoS Pathog 11:e1004868.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  17. 17.

    Chatzinasiou E, Dovas CI, Papanastassopoulou M, Georgiadis M, Psychas V, Bouzalas I, Koumbati M, Koptopoulos G, Papadopoulos O (2010) Assessment of bluetongue viraemia in sheep by real-time PCR and correlation with viral infectivity. J Virol Methods 169:305–315

    CAS  Article  Google Scholar 

  18. 18.

    Lotos L, Efthimiou K, Maliogka VI, Katis NI (2014) Generic detection of poleroviruses using an RT-PCR assay targeting the RdRp coding sequence. J Virol Methods 198:1–11

    CAS  Article  Google Scholar 

  19. 19.

    Stevens M, Freeman B, Liu H-Y, Herrbach E, Lemaire O (2005) Beet poleroviruses: close friends or distant relatives? Publications from USDA-ARS/UNL Faculty. 1445

  20. 20.

    Beuve M, Stevens M, Liu HY, Wintermantel WM, Hauser S, Lemaire O (2008) Biological and molecular characterization of an American sugar beet-infecting Beet western yellow virus isolate. Plant Dis 92:51–60

    CAS  Article  Google Scholar 

  21. 21.

    Lim S, Yoo RH, Igori D, Zhao F, Kim KH, Moon JS (2015) Genome sequence of a recombinant Brassica yellows virus infecting Chinese cabbage. Arch Virol 160:597–600

    CAS  Article  Google Scholar 

  22. 22.

    Martin DP, Murrell B, Golden M, Khoosal A, Muhire B (2015) RDP4: detection and analysis of recombination patterns in virus genomes. Virus Evol 1:vev003.

    Article  PubMed  PubMed Central  Google Scholar 

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CGO, NIK, JTT and VIM designed the study, CGO, GT and SKS performed the experiments, CGO and LL analyzed the data, CGO, JTT, NIK, LL and VIM wrote and edited the manuscript.

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Correspondence to V. I. Maliogka.

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Orfanidou, C.G., Lotos, L., Tsiolakis, G. et al. Molecular characterization of poleroviruses isolated from oilseed rape in Greece. Virus Genes (2021).

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  • Oilseed rape
  • Poleroviruses
  • Molecular characterization
  • Turnip yellows virus
  • Brassica yellows virus
  • Recombination