First complete genome sequence of an isolate of cowpea severe mosaic virus from South America

Virus Genes volume 57, pages238–241(2021)Cite this article

Abstract

In this study, the complete nucleotide sequence of a Brazilian isolate of cowpea severe mosaic virus (CPSMV) is presented for the first time. To date, the CPSMV-DG isolate, from the USA, is the only one with the complete known genome. High-throughput sequencing (Illumina HiSeq) and Sanger sequencing of the total RNA extract from a cowpea plant collected in Teresina city, Brazil, revealed the genome sequence of the CPSMV-Ter1 isolate. RNA-1 and RNA-2 are, respectively, 5921 and 3465 nucleotides (nt) long without the poly(A) tail, and show 77.91% and 76.08% nt sequence identity with CPSMV-DG, considered the type isolate of the species. The open reading frames (ORFs) were determined and the cleavage sites of the polyproteins were predicted. Although the two isolates show a similar genomic organization, there was a low percentage of sequence identity between Ter1 and DG. Furthermore, pairwise comparisons of a partial RNA-1 fragment between CPSMV-Ter1 and 11 CPSMV isolates from Brazil indicated 94.6 to 94.8% nt and 98.9% to 99.4% aa sequence identities.

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Funding

This work was funded by CNPq [JEABJ PQ fellowship]. KJMLT was the recipient of a CAPES scholarship.

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Author notes

  1. Kelvin Josemar M. L. Teixeira and Renan de Souza Cascardo contributed equally to this work.

Affiliations

  1. Dep. de Fitotecnia, Universidade Federal do Piauí, Teresina, PI, 64049-550, Brazil

    Kelvin Josemar M. L. Teixeira & José Evando A. Beserra Jr.

  2. Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil

    Renan de Souza Cascardo, Lorhan L. Leal & F. Murilo Zerbini

Authors
  1. Kelvin Josemar M. L. Teixeira
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  2. Renan de Souza Cascardo
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  3. Lorhan L. Leal
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  4. F. Murilo Zerbini
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  5. José Evando A. Beserra Jr.
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Contributions

Conceptualization, JEABJ and FMZ; investigation, KJMLT, RSC, and LLL; writing—original draft, KJMLT; and writing—review and editing, JEABJ and FMZ.

Corresponding author

Correspondence to José Evando A. Beserra Jr..

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The authors declare that they have no conflicts of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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The sequences reported in this paper have been deposited in the GenBank under accession numbers MW387017 and MW392574.

Edited by Karel Petrzik.

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Cite this article

Teixeira, K.J.M.L., de Souza Cascardo, R., Leal, L.L. et al. First complete genome sequence of an isolate of cowpea severe mosaic virus from South America. Virus Genes 57, 238–241 (2021). https://doi.org/10.1007/s11262-021-01831-2

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Keywords

  • Comovirus
  • CPSMV
  • High-throughput sequencing
  • Secoviridae
  • Vigna unguiculata