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Identification and characterization of a novel bat polyomavirus in Japan

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Abstract

A novel polyomavirus (PyV) was identified in the intestinal contents of Japanese eastern bent-wing bats (Miniopterus fuliginosus) via metagenomic analysis. We subsequently sequenced the full genome of the virus, which has been tentatively named Miniopterus fuliginosus polyomavirus (MfPyV). The nucleotide sequence identity of the genome with those of other bat PyVs was less than 80%. Phylogenetic analysis revealed that MfPyV belonged to the same cluster as PyVs detected in Miniopterus schreibersii. This study has identified the presence of a novel PyV in Japanese bats and provided genetic information about the virus.

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Acknowledgements

This study was partly supported by a Grant (“International joint research and training of young researchers for zoonosis control in the globalized world”) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan.

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Authors and Affiliations

  1. Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan

    Ryota Koba, Satori Suzuki, Go Sato & Yukinobu Tohya

  2. Laboratory of Veterinary Public Health, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan

    Shingo Sato & Soichi Maruyama

  3. Hikiiwa Park Center, 1629 Inari-cho, Tanabe, Wakayama, 646-0051, Japan

    Kazuo Suzuki

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  1. Ryota Koba
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  2. Satori Suzuki
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  3. Go Sato
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  4. Shingo Sato
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  5. Kazuo Suzuki
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  6. Soichi Maruyama
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  7. Yukinobu Tohya
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Correspondence to Yukinobu Tohya.

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11262_2020_1789_MOESM1_ESM.docx

Supplementary file1 (DOCX 1731 kb) Supplementary Fig. 1 Overlap region of VP1 and VP2 from bat polyomaviruses. The start codons of VP2 and stop codons of VP1 are indicated by bold text. The guanine between the stop codon of VP1 and start codon of VP2 in the Miniopterus fuliginosus polyomavirus (MfPyV) sequence is shown in the gray box. Supplementary Fig. 2 Amino acid sequence alignment of the large T antigen N-terminus of bat polyomaviruses. Functional motifs are indicated in gray boxes. MfPyV, Miniopterus fuliginosus polyomavirus. Supplementary Fig. 3 Sequence alignment of the noncoding control region of bat PyVs. The large T antigen binding element (GAGGC) is indicated in bold, and the complementary motif (GCCTC) is presented in the gray box. The putative TATA-box motifs are shown in white boxes. MfPyV, Miniopterus fuliginosus polyomavirus.

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Koba, R., Suzuki, S., Sato, G. et al. Identification and characterization of a novel bat polyomavirus in Japan. Virus Genes 56, 772–776 (2020). https://doi.org/10.1007/s11262-020-01789-7

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  • DOI: https://doi.org/10.1007/s11262-020-01789-7

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