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Isolation and phylogenomic analysis of two Senecavirus A isolates in Sichuan Province, 2018

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Abstract

In our study, we isolated and characterised two new Senecavirus A (SVA) isolates in Sichuan Province, China. Phylogenetic analysis of both the SVA full-length genomes and the VP1 genes revealed that the two new SVA isolates are more closely related to previous Chinese strains and US strains. The most variable isolate, SVV-SC-01, showed a significant difference from previous SVA strains, and it was identified as a recombinant using several algorithms. Study findings indicate that the SVA virus in China is constantly evolving and new SVA variants may have emerged. Hence, we must take effective measures to prevent further spread of SVA. This report provides evidence that SVA infection of pigs has occurred in Sichuan Province, and the results will contribute to our understanding of the genetic characteristics and recombinant events of SVA in China.

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Acknowledgements

This research was funded by the National Key Research and Development Programme (2018YFD0500802), the Science and Technology Programme of Sichuan Province (2018JY0369) and the Sichuan Veterinary Medicine and Drug Innovation Group of the China Agricultural Research System (CARS-SVDIP).

Funding

The Sichuan Veterinary Medicine and Drug Innovation Group of the China Agricultural Research System, Grant/Award Number: CARS-SVDIP.

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Authors and Affiliations

  1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China

    Kenan Peng, Xinhuan Yin, Yingqi Chen, Zhiwen Xu, Jun Zhao, Jianbo Huang, Chaoyuan Jiang & Ling Zhu

  2. Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu, China

    Zhiwen Xu & Ling Zhu

Authors
  1. Kenan Peng
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  2. Xinhuan Yin
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  3. Yingqi Chen
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  4. Zhiwen Xu
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  5. Jun Zhao
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  6. Jianbo Huang
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  7. Chaoyuan Jiang
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  8. Ling Zhu
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Contributions

This work was designed, conducted, and written by KP and YC. XY, JZ, JH and CJ helped in virus isolation and data analysis. ZX and LZ contributed materials and reagents, supervised the work and evaluated the manuscript.

Corresponding author

Correspondence to Ling Zhu.

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Conflict of interest:

The authors declare no conflict of interest.

Ethical approval:

All applicable international, national and institutional guidelines for the use and care of animals were followed.

Additional information

Edited by Zhen F. Fu.

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The genomes of the viruses described in detail here were deposited in GenBank under the following Accession Numbers: MH716015 (isolate SVV-SC-01) and MN700930 (isolate SVV-SC-MS).

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Peng, K., Yin, X., Chen, Y. et al. Isolation and phylogenomic analysis of two Senecavirus A isolates in Sichuan Province, 2018. Virus Genes 56, 785–791 (2020). https://doi.org/10.1007/s11262-020-01788-8

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  • DOI: https://doi.org/10.1007/s11262-020-01788-8

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