Air potato (Dioscorea bulbifera) plants displaying virus-like symptoms are co-infected with a novel potyvirus and a novel ampelovirus

Abstract

Air potato (Dioscorea bulbifera) plants being grown at the Florida Department of Agriculture and Consumer Services Division of Plant Industry Biological Control Laboratory II in Alachua County, Florida were observed exhibiting foliar mosaic symptoms characteristic of virus infection. A double-stranded RNA library generated from a symptomatic plant underwent high-throughput sequencing to determine if viral pathogens were present. Sequence data revealed the presence of two viral genomes, one with properties congruent with members of the genus Potyvirus (family Potyviridae), and the other with members of the genus Ampelovirus (family Closteroviridae). Sequence comparisons and phylogenetic placement indicate that both viruses represent novel species. The names “dioscorea mosaic virus” and “air potato virus 1” are proposed for the potyvirus and ampelovirus, respectively.

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Acknowledgements

The work in Hawaii was supported by the USDA National Institute of Food and Agriculture Hatch Project HAW09030-H awarded to M.J. Melzer and managed by the University of Hawaii’s College of Tropical Agriculture and Human Resources.

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Correspondence to Michael J. Melzer.

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The authors declare that there is no conflicts of interest associated with this study.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Edited by Seung-Kook Choi.

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Dey, K.K., Sugikawa, J., Kerr, C. et al. Air potato (Dioscorea bulbifera) plants displaying virus-like symptoms are co-infected with a novel potyvirus and a novel ampelovirus. Virus Genes 55, 117–121 (2019). https://doi.org/10.1007/s11262-018-1616-6

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Keywords

  • Air potato
  • Ampelovirus
  • Potyvirus
  • High-throughput sequencing
  • Genome characterization
  • Plant virus