Skip to main content
SpringerLink
Log in

Air potato (Dioscorea bulbifera) plants displaying virus-like symptoms are co-infected with a novel potyvirus and a novel ampelovirus

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

Air potato (Dioscorea bulbifera) plants being grown at the Florida Department of Agriculture and Consumer Services Division of Plant Industry Biological Control Laboratory II in Alachua County, Florida were observed exhibiting foliar mosaic symptoms characteristic of virus infection. A double-stranded RNA library generated from a symptomatic plant underwent high-throughput sequencing to determine if viral pathogens were present. Sequence data revealed the presence of two viral genomes, one with properties congruent with members of the genus Potyvirus (family Potyviridae), and the other with members of the genus Ampelovirus (family Closteroviridae). Sequence comparisons and phylogenetic placement indicate that both viruses represent novel species. The names “dioscorea mosaic virus” and “air potato virus 1” are proposed for the potyvirus and ampelovirus, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

References

  1. Kehoe MA, Coutts BA, Buirchell BJ, Jones RAC (2014) Split personality of a potyvirus: to specialize or not to specialize? PLoS ONE 9(8):e105770

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Wylie SJ, Adams M, Chalam C, Kreuze J, López-Moya JJ, Ohshima K, Praveen S, Rabenstein F, Stenger D, Wang A, Zerbini FM, ICTV Report Consortium (2017) ICTV virus taxonomy profile: Potyviridae. J Gen Virol 98:352–354

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Revers F, Garcia JA (2015) Molecular biology of potyviruses. Adv Virus Res 92:101–199

    Article  CAS  PubMed  Google Scholar 

  4. Simmons HE, Dunham JP, Zinn KE, Munkvold GP, Holmes EC, Stephenson AG (2013) Zucchini yellow mosaic virus (ZYMV, Potyvirus): vertical transmission, seed infection and cryptic infections. Virus Res 176:259–264

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Martelli GP, Agranovsky AA, Bar-Joseph M, Boscia D, Candresse T, Coutss RHA, Dolja VV, Falk BW, Gonsalves D, Jelkmann W, Karasev AV, Minafra A, Namba S, Vetten HJ, Wisler GC, Yoshikawa N (2002) The family Closteroviridae revised. Arch Virol 147:2039–2044

    Article  CAS  PubMed  Google Scholar 

  6. Martelli GP, Abou Ghanem-Sabanadzovic N, Agranovsky AA, Al Rwahnih M, Dolja VV, Dovas CI, Fuchs M, Gugerli P, Hu JS, Jelkmann W, Katis NI, Maliogka VI, Melzer MJ, Menzel W, Minafra A, Rott ME, Rowhani A, Sabanadzovic S, Saldarelli P (2012) Taxonomic revision of the family Closteroviridae with special reference to the grapevine-leafroll-associated members of the genus Ampelovirus and the putative species unassigned to the family. J Plant Pathol 94:7–19

    Google Scholar 

  7. Overholt WA, Markle L, Meisenberg M, Raz L, Wheeler G, Pemberton R, Taylor J, King M, Schmitz D, Parks GR, Rayamajhi M, Rohrig E, Lake E, Smith M, Hibbard K, Center TD, Manrique V, Diaz R, Dray FA (2014) Air potato management plan for Florida. Florida Exotic Pest Plant Council. http://www.fleppc.org

  8. Morris TJ, Dodds JA (1979) Isolation and analysis of double-stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854–858

    Article  CAS  Google Scholar 

  9. Melzer MJ, Borth WB, Sether DM, Ferreira S, Gonsalves D, Hu JS (2010) Genetic diversity and evidence for recent modular recombination in Hawaiian Citrus tristeza virus. Virus Genes 40:111–118

    Article  CAS  PubMed  Google Scholar 

  10. Ho T, Tzanetakis IE (2014) Development of a virus detection and discovery pipeline using next generation sequencing. Virology 471–473:54–60

    Article  CAS  PubMed  Google Scholar 

  11. Coutts RHA, Livieratos IC (2003) A rapid method for sequencing the 5′- and 3′-termini of double-stranded RNA viral templates using RLM-RACE. J Phytopathol 151:525–527

    Article  CAS  Google Scholar 

  12. Dey KS, Melzer MJ, Kerr C, Li C, Sun X, Adkins ST (2017) A new potyvirus found in Dioscorea bulbifera in Florida (Abstr.). Phytopathology 107:S5.101

    Google Scholar 

  13. Chung BYW, Miller WA, Atkins JF, Firth AE (2008) An overlapping essential gene in the Potyviridae. Proc Natl Acad Sci USA 105:5897–5902

    Article  CAS  PubMed  Google Scholar 

  14. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23:2947–2948

    Article  CAS  Google Scholar 

  15. Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

    Article  CAS  Google Scholar 

  16. Karasev AV (2000) Genetic diversity and evolution of closteroviruses. Annu Rev Phytopathol 38:293–324

    Article  CAS  PubMed  Google Scholar 

  17. Karyeija RF, Kreuze JF, Gibson RW, Valkonen JPT (2000) Synergistic interactions of a potyvirus and a phloem-limited crinivirus in sweet potato plants. Virology 269:26–36

    Article  CAS  PubMed  Google Scholar 

  18. Center TD, Rayamajhi M, Dray FA, Madeira PM, Witkus G, Rohrig E, Mattison E, Lake E, Smith M, Zhang J, Purcell M, Konstantinov A, Schmitz D (2013) Host range validation, molecular identification and release and establishment of a Chinese biotype of the Asian leaf beetle Lilioceris cheni (Coleoptera: Chrysomelidae: Criocerinae) for control of Dioscorea bulbifera L. in the southern United States. Biocontrol Sci Technol 23:735–755

    Article  Google Scholar 

Download references

Acknowledgements

The work in Hawaii was supported by the USDA National Institute of Food and Agriculture Hatch Project HAW09030-H awarded to M.J. Melzer and managed by the University of Hawaii’s College of Tropical Agriculture and Human Resources.

Author information

Author notes

  1. Kishore K. Dey and Jaylinn Sugikawa have contributed equally to this work.

Authors and Affiliations

  1. Division of Plant Industry, Florida Department of Agriculture and Consumer Services, Gainesville, FL, 32608, USA

    Kishore K. Dey & Christopher Kerr

  2. Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI, 96822, USA

    Jaylinn Sugikawa & Michael J. Melzer

Authors
  1. Kishore K. Dey
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jaylinn Sugikawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Christopher Kerr
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Michael J. Melzer
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Michael J. Melzer.

Ethics declarations

Conflict of interest

The authors declare that there is no conflicts of interest associated with this study.

Research involving human and animal participants

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Edited by Seung-Kook Choi.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Dey, K.K., Sugikawa, J., Kerr, C. et al. Air potato (Dioscorea bulbifera) plants displaying virus-like symptoms are co-infected with a novel potyvirus and a novel ampelovirus. Virus Genes 55, 117–121 (2019). https://doi.org/10.1007/s11262-018-1616-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-018-1616-6

Keywords

Search

Navigation