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Amsacta moorei entomopoxvirus encodes a functional heparin-binding glycosyltransferase (AMV248)

Abstract

Amsacta moorei entomopoxvirus (AMEV) infects certain lepidopteran and orthopteran insects and is the most studied member of the genus Betaentomopoxvirus. It has been considered as a potential vector for gene therapy, a vector to express exogenous proteins and a biological control agent. One of its open reading frames, amv248, encodes a putative glycosyltransferase and is the only known attachment protein conserved in AMEV and chordopoxviruses. The ORF was successfully expressed and the protein was shown to bind soluble heparin, both in silico and in vitro. Our results also showed that, while viral infection was inhibited by soluble glycosaminoglycans (GAGs), GAG-deficient cells were more resistant to the virus. Finally, we revealed that amv248 encodes an active heparin-binding glycosyltransferase which is likely to have a key role in the initiation of infection by AMEV.

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Acknowledgements

This work was supported financially by The Scientific and Technological Research Council of Turkey, TUBITAK, [Project No. 113Z219].

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Authors

Contributions

ZD designed the study. CI and HM performed laboratory work. BMA analyzed the data. All authors contributed to the writing of the manuscript.

Corresponding author

Correspondence to Zihni Demirbag.

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The authors declare that they have no conflict of interest.

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This article does not include any studies with human participants or animals performed by any of the authors.

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Informed consent was obtained from all individual participants included in this article.

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Edited by Lorena Passarelli.

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Inan, C., Muratoglu, H., Arif, B.M. et al. Amsacta moorei entomopoxvirus encodes a functional heparin-binding glycosyltransferase (AMV248). Virus Genes 54, 438–445 (2018). https://doi.org/10.1007/s11262-018-1561-4

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Keywords

  • Amsacta moorei entomopoxvirus
  • Glycosyltransferase
  • amv248
  • Heparin binding
  • Virus attachment