We report here the complete genome sequence of a bipartite virus, herein denoted WLPRV/human/BRA/TO-34/201, from a sample collected in 2015 from a two-year-old child in Brazil presenting acute gastroenteritis. The virus has 98–99% identity (segments 2 and 1, respectively) with the Wuhan large pig roundworm virus (unclassified RNA virus) that was recently discovered in the stomachs of pigs from China. This is the first report of a Wuhan large pig roundworm virus detected in human specimens, and the second genome described worldwide. However, the generation of more sequence data and further functional studies are required to fully understand the ecology, epidemiology, and evolution of this new unclassified virus.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
M. Shi, Y.Z. Zhang, E.C. Holmes, Meta-transcriptomics and the evolutionary biology of RNA viruses. Virus Res. 243, 83–90 (2018)
M. Shi, X.D. Lin, J.H. Tian, L.J. Chen, X. Chen, C.X. Li, X.C. Qin, J. Li, J.P. Cao, J.S. Eden, J. Buchmann, W. Wang, J. Xu, E.C. Holmes, Y.Z. Zhang, Redefining the invertebrate RNA virosphere. Nature 540, 539 (2016)
L. Li, X. Deng, E.T. Mee, S. Collot-Teixeira, R. Anderson, S. Schepelmann, P.D. Minor, E. Delwart, Comparing viral metagenomics methods using a highly multiplexed human viral pathogens reagent. J. Virol. Methods 213, 139–146 (2015)
A.C. da Costa, J. Thézé, S.C.V. Komninakis, R.L. Sanz-Duro, M.R.L. Felinto, L.C.C. Moura, I.M.O. Barroso, L.E.C. Santos, M.A.L. Nunes, A.A. Moura, J. Lourenço, X. Deng, E.L. Delwart, M.R.D.A.S. Guimarães, O.G. Pybus, E.C. Sabino, N.R. Faria, Spread of chikungunya virus East/Central/South African genotype in Northeast Brazil. Emerg. Infect. Dis. 23(10), 1742–1744 (2017). https://doi.org/10.3201/eid2310.170307
X. Deng, S.N. Naccache, T. Ng, S. Federman, L. Li, C.Y. Chiu, E.L. Delwart, An ensemble strategy that significantly improves de novo assembly of microbial genomes from metagenomic next-generation sequencing data. Nucleic Acids Res. 43(7), e46 (2015)
A.J. Drummond, M.A. Suchard, D. Xie, A. Rambaut, Bayesian phylogenetics with BEAUti and the BEAST 1.7. Mol. Biol. Evol. 29(8), 1969–1973 (2012). https://doi.org/10.1093/molbev/mss075
S. Kumar, G. Stecher, K. Tamura, MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 33(7), 1870–1874 (2016). https://doi.org/10.1093/molbev/msw054
We are grateful to Zymo Research Inc, Illumina Inc., Sage Science Inc., Promega Biotecnologia do Brasil Ltda, for the donation of reagents for this project.
Adriana Luchs is funded by FAPESP #2015/12944-9, and Antônio Charlys da Costa by FAPESP #2017/00021-9. This work was also supported by FAPESP #2016/01735-2 and CNPq #400354/2016-0.
Conflict of interest
The authors declare that they have no conflict of interest.
Previous Ethics Committee approval was granted by Adolfo Lutz Institute, São Paulo, Brazil (CEP 965.723; CTC 45G-2014), Faculdade de Medicina da Universidade de São Paulo (CAAE: 53153916.7.0000.0065), and Centro Universitário Luterano de Palmas—ULBRA (CAAE 53153916.7.3007.5516). This was an anonymous unlinked study, and informed consent was not required according to resolution 466/12 concerning research involving humans (Conselho Nacional de Saúde/Ministério da Saúde, Brasília, 2012).
Accession number The complete bisegmented genome sequence of WLPRV/human/BRA/TO-34/2015 strain has been deposited in GenBank under accession numbers MG746618 (segment 1) and MG746619 (segment 2).
Edited by William Dundon.
About this article
Cite this article
Luchs, A., Leal, E., Komninakis, S.V. et al. Wuhan large pig roundworm virus identified in human feces in Brazil. Virus Genes 54, 470–473 (2018). https://doi.org/10.1007/s11262-018-1557-0
- Virus discovery
- Wuhan large pig roundworm virus