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Proteomic analysis of A-549 cells infected with human adenovirus 40 by LC-MS

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Abstract

Human Adenoviruses (HAdVs) are etiological agents of different syndromes such as gastroenteritis, cystitis, ocular, and respiratory diseases, and infection by these viruses may cause alterations in cellular homeostasis. The objective of the study was the proteomic analysis of A-549 cells infected with HAdV-40 using LC–MS. At 30 h of infection, the quantitative analysis revealed 336 differentially expressed proteins. From them, 206 were induced (up-regulated) and 130 were suppressed (down-regulated). The majority of up-regulated proteins were related to energy, cellular organization, stress response, and apoptosis pathways. It was observed alteration of cell metabolism with increase of the glycolytic pathway, β-oxidation, and respiratory chain. Also, the results suggest cytoskeleton reorganization and apoptosis induction. The data can improve knowledge about the replication of HAdV-40 in cell culture considering the proteins related to distinct metabolic pathways induced by viral infection in A-549 cells.

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Acknowledgements

This work was supported by Pronex announcement MCT/CNPQ/FNDCT/FAPEG/MEC/CAPES/PRO CENTRO-OESTE nº 031/2010, Process nº 563998/2010–5.

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Authors and Affiliations

  1. Human Virology Laboratory, Institute of Tropical Pathology and Public Health, Federal University of Goias, Rua 235, S/N, Sala 418, Setor Universitário, Goiania, Goias, 74605050, Brazil

    Ana Carla Peixoto Guissoni, Kareem R. Badr, Fabiola Sousa Ficcadori, Menira Souza & Divina das Dores de Paula Cardoso

  2. Molecular Biology Laboratory, Institute of Biological Sciences, Federal University of Goias, Goiania, Goias, Brazil

    Célia Maria Almeida Soares, Ana Flávia Alves Parente, Juliana Alves Parente & Lilian Cristina Baeza

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  1. Ana Carla Peixoto Guissoni
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All authors contributed equally to this work

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Correspondence to Divina das Dores de Paula Cardoso.

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The study was approved by the Ethics Committee of the Federal University of Goias (protocol 136/12).

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Edited by Hartmut Hengel.

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Guissoni, A.C.P., Soares, C.M.A., Badr, K.R. et al. Proteomic analysis of A-549 cells infected with human adenovirus 40 by LC-MS. Virus Genes 54, 351–360 (2018). https://doi.org/10.1007/s11262-018-1554-3

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