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Virus Genes

, Volume 54, Issue 3, pp 385–396 | Cite as

Proteome analysis of differential protein expression in porcine alveolar macrophages regulated by porcine reproductive and respiratory syndrome virus nsp1β protein

  • Yinghao Xin
  • Dang Wang
  • Meijin Huang
  • Jinjin Yu
  • Liurong Fang
  • Shaobo XiaoEmail author
Article
  • 431 Downloads

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV), an acute infectious disease agent in swine, causes enormous economic losses to the global swine industry. PRRSV nonstructural protein 1β (nsp1β) plays a critical role in viral subgenomic mRNA synthesis and host immune regulation. However, the global changes of cellular gene expression in natural target cells regulated by the nsp1β have not yet been identified. Here, isobaric tags for relative and absolute quantification (iTRAQ) labeling coupled with liquid chromatography-tandem mass spectrometry were used to quantitatively identify cellular proteins in porcine alveolar macrophage (PAM) 3D4/21 cells transduced with recombinant lentivirus expressing PRRSV nsp1β that are differentially expressed compared with PAM 3D4/21 cells transduced with recombinant lentivirus expressing GFP. Of the 425 cellular proteins detected as differentially expressed, 186 were upregulated and 239 were downregulated. Based on the identities of the differentially expressed cellular proteins and the essential role of nsp1β in interferon (IFN) activation and inflammatory factor antagonism during PRRSV infection, we propose a potential mechanism in which nsp1β inhibits IFN induction and nuclear factor κB (NF-κB) signaling pathways. Our results suggest that mitochondrial antiviral signaling (MAVS) protein and translocases of outer membrane complex 70 (TOM70), involved in type I IFN induction, were downregulated, while protein phosphatase 1A (PPM1A), related to the inhibition of NF-κB pathway activation, was upregulated in nsp1β-overexpressed PAM 3D4/21 cells. These data provide valuable information for better understanding the potential biological function of nsp1β during PRRSV infection and the mechanism of virus escape from host immune surveillance of viral replication.

Keywords

Porcine reproductive and respiratory syndrome virus (PRRSV) nsp1β iTRAQ Quantitative proteomics NF-κB 

Notes

Acknowledgements

This work was supported by the National Basic Research Program (973) of China (2014CB542700), the National Natural Sciences Foundation of China (31490602, 31372467).

Author contributions

YX, MH, and SX designed the experiments. YX, MH, and DW performed the experiments. MH and JY contributed data analysis. YX, MH, and DW wrote the manuscript. LF and SX revised the manuscript content.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

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Supplementary material 4 (DOCX 380 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Yinghao Xin
    • 1
    • 2
  • Dang Wang
    • 1
    • 2
  • Meijin Huang
    • 1
    • 2
  • Jinjin Yu
    • 1
    • 2
  • Liurong Fang
    • 1
    • 2
  • Shaobo Xiao
    • 1
    • 2
    • 3
    Email author
  1. 1.State Key Laboratory of Agricultural Microbiology, College of Veterinary MedicineHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  2. 2.The Key Laboratory of Preventive Veterinary Medicine in Hubei ProvinceCooperative Innovation Center for Sustainable Pig ProductionWuhanPeople’s Republic of China
  3. 3.Laboratory of Animal Virology, College of Veterinary MedicineHuazhong Agricultural UniversityWuhanPeople’s Republic of China

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