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Virus Genes

, Volume 53, Issue 6, pp 906–912 | Cite as

The efficacy of antisense-based construct for inducing resistance against Croton yellow vein mosaic virus in Nicotiana tabacum

  • V. SinhaEmail author
  • N. B. Sarin
  • D. Bhatnagar
Article
  • 163 Downloads

Abstract

Begomoviruses have increased pathogenicity because of their adaptation to a wide host range; consequently, these viruses cause a major loss to agroeconomic crops worldwide. In this study, we designed a gene construct representing an antisense coat protein gene. We also analyzed the efficacy of the induced resistance against Croton yellow vein mosaic virus (CrYVMV) affecting papaya in Nicotiana tabacum plants. Positive control plants developed typical leaf curl symptoms, whereas transgenic plants were symptomless. Moreover, the key component (i.e., short interfering RNA) of the antisense pathway was upregulated in transgenic plants. This finding demonstrates the activation of the gene silencing mechanism in transgenic plants. Thus, these results confirm that our construct is functional and effectively induces transient resistance against CrYVMV infections.

Keywords

Begomovirus Antisense CrYVMV Coat protein siRNA 

Notes

Acknowledgements

Authors are thankful to UGC for funding to the lab.

Author contributions

DB helped in experimental designing. VS has done all the experimental part. NBS supervised this study and gave final approval for manuscript submission.

Compliance with ethical standards

Conflict of interest

Authors do not have any conflict of interest.

Informed consent

The study was approved by ethical committee of JNU, New Delhi.

Research involving human participants

No animals were used in this study.

References

  1. 1.
    S. Asad, W.A. Haris, A. Bashir, Y. Zafar, K.A. Malik, N.N. Malik, C.P. Lichtenstein, Arch. Virol. 148, 2341–2352 (2003)CrossRefPubMedGoogle Scholar
  2. 2.
    I.S. Afolabi, I.O. Osikoya, O.D. Fajimi, P.I. Usoro, D.O. Ogunleye, T. Bisi-Adeniyi, A.O. Adeniyi, B.T. Adekeye, BMC Complement. Altern. Med. 12, 262 (2012)CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    D.C. Baulcombe, Nature 431, 356–363 (2004)CrossRefPubMedGoogle Scholar
  4. 4.
    R.W. Briddon, J. Stanley. Virol. 344, 198–210 (2006)Google Scholar
  5. 5.
    J.K. Brown, A.M. Idris, I. TorresJerez, G.K. Banks, S.D. Wyatt, Arch. Virol. 146, 1581–1598 (2001)CrossRefPubMedGoogle Scholar
  6. 6.
    R. Dickerson, B. Deshpande, U. Gnyawali, D. Lynch, G. Gordillo, D. Schuster, K. Osei, S. Roy, Antioxid. Redox Signal. 17, 485–491 (2012)CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    J.J. Doyle, J.L. Doyle, Focus 12, 13–15 (1990)Google Scholar
  8. 8.
    R. Hofgen, L. Willmitzer, Nucl. Acid Res. 16, 9877 (1988)CrossRefGoogle Scholar
  9. 9.
    R.B. Horsch, J.E. Fry, N.L. Hoffman, D. Eichholtz, S.G. Rogers, R.T. Fraley, Science 227, 1229–1231 (1985)CrossRefGoogle Scholar
  10. 10.
    A.K. Inoue-Nagata, M.F. Lima, R.L. Gilbertson, Hortic. Bras. 34, 8–18 (2016)CrossRefGoogle Scholar
  11. 11.
    J.A. Khan, J. Ahmad, Curr. Sci. 88, 1803–1809 (2005)Google Scholar
  12. 12.
    J.A. Khan, J. Dijkstra, Plant Viruses as Molecular Pathogens (The Haworth Press, New York, 2006)Google Scholar
  13. 13.
    A. Kumar, S.K. Snehi, S.K. Raj, J. Kumar, J.A. Khan, New Dise. Rep. 25, 9 (2011)CrossRefGoogle Scholar
  14. 14.
    R. Kumria, R. Verma, M.V. Rajam, Curr. Sci. 74, 35–41 (1998)Google Scholar
  15. 15.
    J.A. Lindbo, W.G. Dougherty, Annu. Rev. Phytopathol. 43, 191–204 (2005)CrossRefPubMedGoogle Scholar
  16. 16.
    S. Mansoor, R.W. Briddon, Y. Zafar, J. Stanley, Trends Plant Sci. 8, 128–134 (2003)CrossRefPubMedGoogle Scholar
  17. 17.
    S. Mansoor, S.H. Khan, A. Bashir, M. Saeed, Y. Zafar, K.A. Malik, R.W. Briddon, J. Stanley, P.G. Markham, Virology 259, 190–199 (1999)CrossRefPubMedGoogle Scholar
  18. 18.
    S. Martino-Catt, E.S. Sachs, Plant Physiol. 147, 3–5 (2008)CrossRefPubMedPubMedCentralGoogle Scholar
  19. 19.
    P. Melariri, W. Campbell, P. Etusim, P. Smith, J. Parasitol. Res. (2011). doi: 10.1155/2011/10495 PubMedPubMedCentralGoogle Scholar
  20. 20.
    M. Mishra, N. Shukla, R. Chandra, Protocols for micropropagation of woody trees and fruits (Springer, New York, 1996), pp. 437–441Google Scholar
  21. 21.
    E. Panzarini, M. Dwikat, S. Mariano, C. Vergallo, L. Dini, Evid. Comp. Altern. Med. (2014). doi: 10.1155/2014/281508 Google Scholar
  22. 22.
    P. Powell-Abel, R.S. Nelson, B. De, N. Hoffman, S.G. Rogers, R.T. Fraley, R.N. Beachy, Science 232, 738–743 (1986)CrossRefGoogle Scholar
  23. 23.
    J. Sambrook, E.F. Fritsch, T. Maniatis, Molecular Cloning—A Laboratory Manual (Cold Spring harbour Laboratory, New York, 1989)Google Scholar
  24. 24.
    J. Sambrook, D.W. Russel, Molecular Cloning: A laboratory manual, 3rd edn. (Cold Spring Harbor Laboratory Press, New York, 2001)Google Scholar
  25. 25.
    V. Sinha, A. Kumar, N.B. Sarin, D. Bhatnagar, Int. J. Adv. Res. 4(3), 1598–1604 (2016)Google Scholar
  26. 26.
    S. Subenthiran, T.C. Choon, K.C. Cheong, R. Thayan, M.B. Teck, Evid-Based Comp. Alt. Med. (2013). doi: 10.1155/2013/616737 Google Scholar
  27. 27.
    P.A. Tarkang, A.P.N. Atchan, J. Kuiate, F.A. Okalebo, A.N. Guantai, G.A. Agbor, Adv. Pharmacol. Sci. 13, 1 (2013)Google Scholar
  28. 28.
    K.M. Thomas, C.S. Krishnaswamy, Curr. Sci. 8, 316 (1939)Google Scholar
  29. 29.
    A. Varsani, D.P. Martin, J. Navas-Castillo, E. Moriones, C. Hernández-Zepeda et al., Arch. Virol. 158, 1873–1882 (2014)CrossRefGoogle Scholar
  30. 30.
    H. Vaucheret, Genes Dev. 20, 759–771 (2006)CrossRefPubMedGoogle Scholar
  31. 31.
    C.R. Wilson, A.J. Doudna, Annl. Rev. Biophy. 42, 217–239 (2013)CrossRefGoogle Scholar
  32. 32.
    Z. Xie, L.K. Johansen, A.M. Gustafson, K.D. Kasschau, A.D. Lellis, PLoS Biol. 2, E104 (2004)CrossRefPubMedPubMedCentralGoogle Scholar
  33. 33.
    P. Yadava, G. Suyal, S.K. Mukherjee, Curr. Sci. 98, 360–368 (2010)Google Scholar
  34. 34.
    M. Yusha’u, F.C. Onuorah, Y. Murtala, J. Pure. Appl. Sci. 2, 75–78 (2009)Google Scholar
  35. 35.
    D. Zilberman, X. Cao, L.K. Johansen, Z. Xie, J.C. Carrington, S.E. Jacobsen, Curr. Biol. 14, 1214–1220 (2004)CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  1. 1.School of Life SciencesJawaharlal Nehru UniversityNew DelhiIndia
  2. 2.School of BiochemistryDevi Ahilya UniversityIndoreIndia

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